Abstract: The fungi chaff of Pleurotus geesteranus were used to explore the spray drying conditions of laccase extract. And laccase preparation were optimized by orthogonal tests on the basis of single factor experiments. The results indicated that the optimal extraction conditions were crush time of 30 s,solid-liquid ratio of 1∶10（g∶mL）,extraction time of 30 min,shaking speed of 160 r/min and extraction temperature of 25 ℃. The optimal spray drying conditions were as follows：inlet air temperature 95 ℃,flux rate 20 mL/min,hot air flow 3.5 m³/min. This study would provide a reference basis for the production of large scale production of laccase from Pleurotus geesteranus chaff.

Key words: Pleurotus geesteranus, fungi chaff, laccase, spray drying, enzyme preparation