Abstract: Tamarix chinensis Lour. is a salt-tolerance shrub or small tree,scattered in the riverbed or forming large natural populations in coastal tidal areas. For population genetics study of this species,the orthogonal design was used to determine the PCR amplification system of genomic SSR derived from Illumina sequencing in T. chinensis. The 10 μL reaction system of gSSR is as follows：0.8 mmol/L Mg²⁺,0.25 mmol/L dNTPs,0.5 U Taq DNA polymerase,0.5 μmol/L primers,and 20 ng DNA.

Key words: Tamarix chinensis Lour., genomic microsatellites, PCR reaction system, orthogonal design