Effects of Azadirachtin on Respiration and Protective Enzymes Activity of Tenebrio molitor Larvae

doi:10.14088/j.cnki.issn0439-8114.2018.08.014

Abstract

Abstract: To investigate the effects of botanical pesticide on the respiration and protective enzymes of coleopteran,CO₂ release rate and protective enzymes in the 8th instar larvae of Tenebrio molitor fed with azadirachtin were examined. The results showed that the CO₂ release rate and protective enzymes of T. molitor fed with azadirachtin increased initially and then declined rapidly. The highest CO₂ release rate of T. molitor was 13.2 μL/h after 6 hours of feeding with azadirachtin and then fell quickly,remarkably lower than the value of control groups. The maximum activities of protective enzymes including SOD,CAT and POD appeared after 6 hours of feeding with azadirachtin, which were 2.34,2.91 and 1.98 times than those in the control group,respectively. And these protect enzymes lowered fast subsequently.

Key words: azadirachtin, Tenebrio molitor, respiratory, protective enzymes

CLC Number:

S433

GUO Chun-hui, NING Chang-long, WANG Rui-rui, WU Guang, ZHAO Peng-fei, YANG Bing-ye, YANG Zhen-de. Effects of Azadirachtin on Respiration and Protective Enzymes Activity of Tenebrio molitor Larvae[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(8): 55-57.

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