Detection Amino Acids in Muscle of Hypophthalmichthys molitrix,Aristichthys nobilis and Ctenopharyngodon idellus by HPLC

doi:10.14088/j.cnki.issn0439-8114.2018.13.022

Abstract

Abstract: The experiment was established by HPLC method for detection of amino acids in the muscle of aquatic products. Amino acids in samples were separated by Eelipse-AAA column. Aboric acid buffer solution as pH regulator,with two formaldehyde phthalate （OPA） and 9-fluorene methyl chloroformate （FMOC） as a precolumn derivatization reagent,the mobile phase was two sodium hydrogen phosphate,methanol,acetonitrile and water,the column temperature was 40 ℃. At the same time using ultraviolet detector （DAD） and fluorescence detector（FLD） were detected in the same sample,comparison the LOD of two kinds detectors,and determination of the content of amino acids by the results of the two detectors. The results showed that 17 kinds of amino acids could be separated well with ultraviolet detector（DAD） in 26 min,and 16 amino acids could be separated with the fluorescence detector in 26 min. The essential amino acids of Hypophthalmichthys molitrix,Aristichthys nobilis and Ctenopharyngodon idellus accounted for the proportion of total amino acids were 35.74%,34.67%,36.24%.

Key words: hypophthalmichthys molitrix, aristichthys nobilis, ctenopharyngodon idellus, amino acids, HPLC, precolumn derivatization

CLC Number:

O657.7⁺2

LI Yan, XIE Yong, YANG Fa-hui. Detection Amino Acids in Muscle of Hypophthalmichthys molitrix,Aristichthys nobilis and Ctenopharyngodon idellus by HPLC[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(13): 79-83.

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