HUBEI AGRICULTURAL SCIENCES ›› 2018, Vol. 57 ›› Issue (16): 66-69.doi: 10.14088/j.cnki.issn0439-8114.2018.16.015

• Plant Protection • Previous Articles     Next Articles

Preservation of Liquid Formulation of the Biocontrol Agent Candida intermedia C410

LI Ruo-chen, ZHANG Jing, YANG Long, LI Guo-qing   

  1. College of Plant Science & Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2018-03-30 Published:2019-12-16

Abstract: The preservation of liquid formulation of the biocontrol agent Candida intermedia C410 was primarily explored. Results showed that, while the initial preserving concentration was 2×109 cells/mL, storage temperature was 4 ℃, liquid formulation of C410 with high viability of cells were phosphate buffers supplemented with 5% trehalose and 1 mmol/L ascorbic acid. The liquid formulation kept high viability of yeast cells for more than 90 days. Suspensions of liquid formulation after 90 days and fresh yeast cells were tested against B. cinerea on tomato fruits, both suspensions exhibited prospective biocontrol effect, gray mould of tomato was inhibited while the quality of tomato fruits maintain completely good.

Key words: gray mold, biocontrol, Candida intermedia C410, liquid formulation

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