TRAP analysis of disease and pest resistance related gene SGT1 in sweetpotato

doi:10.14088/j.cnki.issn0439-8114.2019.17.032

Abstract

Abstract: Disease and pest resistance related gene SGT1 was used as a target gene to design a fixed primer,then for paring 11 pairs primers with 11 arbitrary primers. The 11 pairs of primers were used to amplify Eshu 11 and Ezishu 13 by TRAP-PCR. It was found that 11 pairs of primer combinations amplified clear bands and showed good polymorphism. In this study, TRAP markers associated with SGT1 gene were obtained, which can be used to analyze the genetic diversity of sweetpotato[Ipomoea batatas（L.） Lam.] population, and provide theoretical basis for disease and insect resistance breeding of sweetpotato.

Key words: sweetpotato[Ipomoea batatas（L.） Lam.], disease and insect resistance, SGT1 gene, TRAP marker

CLC Number:

Q789
S531

WANG Chong, YANG Xin-sun, LEI Jian, SU Wen-jin, CHAI Sha-sha, ZHANG Wen-ying, WANG Lian-jun. TRAP analysis of disease and pest resistance related gene SGT1 in sweetpotato[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(17): 119-122.

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