The interaction of Lj5NG4 and LjNFR1 in Lotus japonicus regulates the infection of Rhizobium

doi:10.14088/j.cnki.issn0439-8114.2020.05.029

Abstract

Abstract: ： Using the Lotus japonicus nodule factor receptor kinase LjNFR1 as a bait and a yeast membrane two hybrid library constructed with transcripts harvested at different stages of nodule symbiosis, we identified an auxin related transporter protein Lj5NG4. The interaction between Lj5NG4 and LjNFR1 was further confirmed by yeast two hybrid and Split-Luc experiments. Using CRISPR-Cas9 and overexpression technology, we conclude that Lj5NG4 may play a negative regulatory role in the early stage of rhizobial infection, but had no significant effect on nodule formation. Meanwhile, promoter expression also confirmed that Lj5NG4 was strongly expressed in root hairs at the early stage of infection, but not in inner mature nodules. Exogenously applied auxin treatment showed that low concentration of auxin weakened the phenotype of Lj5NG4 mutant, indicating that auxin plays a role in the development of symbiotic nodules.

Key words: Lotus japonicus, rhizobium infection, LjNFR1, Lj5NG4, auxin

CLC Number:

Q789

SONG Jia-bin, DUANMU De-qiang. The interaction of Lj5NG4 and LjNFR1 in Lotus japonicus regulates the infection of Rhizobium[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(5): 145-151.

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