HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (10): 120-122.doi: 10.14088/j.cnki.issn0439-8114.2020.10.028

• Animal Husbandry & Veterinary Medicine • Previous Articles     Next Articles

Establishment of seed batches of avian infectious bronchitis virus

WANG Hong-cai, WANG Hui, WANG Hong-Lin, WEN Guo-yuan, ZHNAG Teng-fei, LUO Ling, LU Qin, ZHANG Rong-rong, LUO Qing-ping   

  1. Institute of Animal Husbandry and Veterinary Sciences,Hubei Academy of Agricultural Sciences/Key Laboratory of Prevention and Control Agents for Animal Bacteriosis of Ministry of Agriculture and Rural Affairs / Hubei Key Laboratory of Animal Embryo and Molecular Breeding,Wuhan 430064,China
  • Received:2019-10-12 Online:2020-05-25 Published:2020-08-03

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Abstract: In order to ensure the quality of infectious bronchitis antigen, the EID50 of infectious bronchitis virus strains from three sources were determined and the virus strains with high titer as virus species were selected, and the seed batch of infectious bronchitis virus WH strain were established. The poison value of freeze-dried seed poison was determined. The results showed that there was no significant difference in the poison value of toxic allantoic fluid before and after freeze-drying, and the EID50 of the virus had no change within 10 generations. The poison value of freeze-dried seed poison remained basically unchanged when it was stored at -40 ℃ and -15 ℃ for one year. The seed batch was not contaminated by bacteria and other microorganisms.

Key words: infectious bronchitis, WH strain, seed batch

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