Progress of small molecule compounds targeting DNA ligase IV to improve the efficiency of CRISPR/Cas9 genome editing

doi:10.14088/j.cnki.issn0439-8114.2020.22.003

Abstract

Abstract: Gene-editing-tools can create double-strand breaks (DSB) at specific sites on the DNA chains.There are two different ways to repair DSB in cells,namely homologous recombination (HDR) and non-homologous end joining (NHEJ).NHEJ pathway and HDR pathway compete with each other as alternative DNA repair pathways.DNA ligase IV acts an important role in the NHEJ pathway.Inhibitors of DNA ligase IV can inhibiting the NHEJ efficiency while improving HDR efficiency in the DNA repair pathway.To optimize small-molecule compounds for more effective site-specific insertion of genes,the studies on the four different chemicals SCR7,NU7026,resveratrol and L755507 were reviewed,and these four small-molecule compounds in gene-editing were summarized.Among them,the bioinformatics software was used to simulate the docking of these four chemicals with DNA ligase IV.Based on these,the small molecule compounds used to improving gene editing efficiency was prospected.

Key words: DSB(double strand break), HDR(Homologous directed recombination), NHEJ (Nonhomologous end joining), DNA ligase IV, small molecule compound

CLC Number:

XIAO Hong-wei, BI Yan-zhen. Progress of small molecule compounds targeting DNA ligase IV to improve the efficiency of CRISPR/Cas9 genome editing[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 13-19.

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