Evaluation and screening of internal reference genes of Tetranychus cinnabarinus at different developmental stages

doi:10.14088/j.cnki.issn0439-8114.2021.11.030

Abstract

Abstract: In the real-time fluorescent quantitative PCR experiments need to introduce one or a few stable reference genes as a reference for expression result correction and standardization, but not all reference genes are perfect for any specific biological samples under special experiment conditions, in order to get accurate results, We need to screen the best reference genes match with the experiment. In this study, real-time fluorescence quantitative PCR and geNorm software were used to analysis and evaluate the stability of six commonly used reference genes in the propargite resistance and sensitive Tetranychus cinnabarinus. The result showed that RPS18 and α-TUB reference genes showed high expression stability in this two strains,and α-TUB and GAPDH reference genes showed high expression stability in different developmental stages.

Key words: Tetranychus cinnabarinus, real-time fluorescence quantitative PCR, reference genes, evaluation and screening

CLC Number:

Q789
S433.7

NI Jing, XIE Dao-yan, JIANG Xiu-jun, YANG Zhen-guo, CHAI Jian-ping. Evaluation and screening of internal reference genes of Tetranychus cinnabarinus at different developmental stages[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 141-145.

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