Isolation, culture and identification of Hubei porcine spermatogonial stem cells

doi:10.14088/j.cnki.issn0439-8114.2021.22.027

Abstract

Abstract: The present study amimed to estabish a ripe system of Hubei white pig SSCs in vitro isolation and culture, in order to lay the foundation for related research. The testes of Hubei white pigs aged 5days, 10 days and 15 days after birth were studied,to obtained the spermatogonial stem cells of Hubei white pig by two-step enzyme digestion（4 mg/mL type Ⅳ collagenase and 0.25% trypsin）. The spermatogonial stem cells of Hubei white pigs were purified by low-speed centrifugation and differential adherence method and cultured in the sertoil cells of Hubei white pig that as feeder layer in vitro. Identified by alkaline phosphatase （AKP） and the results showed that the cultured cells were SSCs.

Key words: Hubei pig, spermatogonial stem cells, testis, alkaline phosphatase

CLC Number:

Q813.1⁺1

ZHANG Li-ping, HUA Zai-dong, XIAO Hong-wei, REN Hong-yan, ZHU Zhe, BI Yan-zhen. Isolation, culture and identification of Hubei porcine spermatogonial stem cells[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(22): 128-132.

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