HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (3): 96-99.doi: 10.14088/j.cnki.issn0439-8114.2022.03.018

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Study on acute toxicity of ethanol extract of Pericampylus glaucus and screening of effective anti-inflammatory and analgesic components

LIU Wen-jun1,2, LI Jin-zhou3, CHEN Zi-jun1, QIN Chun-mei1, ZHOU Meng-ying1, CHEN Yong1   

  1. 1.Scientific Experiment Center of Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;
    2. Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen 518100, Guangdong,China;
    3.Guilin People's Hospital, Guilin 541002, Guangxi,China
  • Received:2021-12-07 Online:2022-02-10 Published:2022-03-11

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Abstract: To study the acute toxicity of alcohol extract of Pericampylus glaucus,the effective anti-inflammatory and analgesic parts of Pericampylus glaucus were screened out to provide theoretical basis for the development of new drugs. The maximum dose assay was used to determine the safety of the water extract and alcohol extract by intragastric administration. The analgesic effects of different extract parts of Pericampylus glaucus were investigated by glacial acetic acid-induced pain in mice and thermal stimulation in mice. The anti-inflammatory effects of different extract parts of Pericampylus glaucus were observed by AGAR granulomatous method. The results showed that in acute toxicity, the lethal dose （LD50） was not measured, the MTD of the alcohol extract was more than 94 g/kg, and the maximum tolerance was 376 times of the adult dosage （60 kg）. The inhibition rates of petroleum ether, ethyl acetate, n-butanol and water were 13.74%, 21.35%, 16.35% and 32.27%, respectively, with statistical significance. Compared with blank control group, the pain threshold of petroleum ether and water parts was significantly increased 90 and 120 min after administration. The pain threshold of ethyl acetate part and n-butanol part of Pericampylus glaucus significantly increased 120 min after administration, and different parts of Pericampylus glaucus could relieve the pain caused by acetic acid in mice. The analgesic rates of petroleum ether part, ethyl acetate part, N-butanol part and water part were 51.74%, 37.63%, 32.13% and 40.55%, respectively. Compared with blank group, the difference was statistically significant. There was no obvious toxicity, and the usual clinical dosage was safe and feasible. In the screening of effective anti-inflammatory and analgesic parts, it was found that the different parts of Pericampylus glaucus had certain anti-inflammatory and analgesic effects, the oil ether part of Pericampylus glaucus had the strongest analgesic effect, and the water part had the strongest anti-inflammatory effect.

Key words: Pericampylus glaucus, acute toxicity experiment, maximum tolerated dose（MTD）, anti-inflammatory and analgesic, valid target

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