HUBEI AGRICULTURAL SCIENCES ›› 2022, Vol. 61 ›› Issue (15): 150-152.doi: 10.14088/j.cnki.issn0439-8114.2022.15.026

• Horticulture & Local Products • Previous Articles     Next Articles

Rapid determination of seed viability of Aconitum kusnezoffii by tetrazolium staining method

SUI Xin1, YU Ying1, LU Hai-kun1, YU Yun-ze1, GUO Jing1, JIANG Ye-cheng2   

  1. 1. Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112,China;
    2. Institute of Resources and Environment, Jilin Academy of Agricultural Sciences, Changchun 130112,China
  • Received:2021-09-16 Online:2022-08-10 Published:2022-08-25

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Abstract: In order to realize the rapid determination of the seed vigor of Aconitum kusnezoffii, through the orthogonal test of 4 factors and 3 levels, the influence of each factor in the seed vigour of Aconitum kusnezoffii on the dyeing effect was explored, and the optimal dyeing conditions were determined. The results showed that the influence of various factors on the seed dyeing effect was ranked as dyeing time>TTC concentration>soaking time>dyeing temperature. The most suitable condition for determining seed viability is after seed soaking for 12 h, staining with 0.3% TTC dye solution at 30 ℃ for 15 h.

Key words: Aconitum kusnezoffii, seed, TTC dyeing method, vitality

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