Research on active components of Lycium chinense and their pharmacological effects

doi:10.14088/j.cnki.issn0439-8114.2022.18.021

Abstract

Abstract: With the continuous deepening of the research on the traditional Chinese medicine Lycium chinense, it is found that its chemical components are rich and diverse, including alkaloids, polysaccharides, flavonoids, phenylpropanoids and so on. Lycium chinense has a variety of pharmacological activities such as liver and kidney protection, anti-oxidation, anti-aging, anti-tumor, neuroprotective activity, anti-inflammatory, antipyretic, hypoglycemic and so on, and its pharmacological effect research is also expanding. In this paper, the chemical constituents and pharmacological effects of Lycium chinense and Lycium barbarum are reviewed, which can provide reference for the value excavation and resource development of Lycium chinense.

Key words: Lycium chinense, chemical constituent, pharmacological activity

CLC Number:

R282

ZOU Xin, CHAN Liu-jia, YI Bing, DING Shi-lei, PEI Dong, WEI Jian-teng, ZHAO Li-chun. Research on active components of Lycium chinense and their pharmacological effects[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(18): 120-130.

References

[1] 董静洲, 杨俊军, 王瑛. 我国枸杞属物种资源及国内外研究进展[J]. 中国中药杂志, 2008(18): 2020-2027.
[2] 如克亚·加帕尔, 孙玉敬, 钟烈州, 等. 枸杞植物化学成分及其生物活性的研究进展[J]. 中国食品学报,2013,13(8):161-172.
[3] (春秋)孔子. 诗经[M]. 于夯,吴京,译,注. 武汉:武汉出版社, 1997. 213.
[4] (南朝·梁)陶弘景. 本草经集注辑校本[M]. 北京:人民卫生出版社,1994.551.
[5] (明)李时珍. 本草纲目[M]. 北京:中国中医药出版社, 1998.1210.
[6] 卫裴, 梁杰, 吴志贤. 枸杞多糖药理学功效研究综述[J]. 辽宁中医药大学学报, 2012, 14(6): 247-249.
[7] 国家药典委员会编. 中华人民共和国药典一部[M]. 北京:中国医药科技出版社, 2020.1902.
[8] 张惠玲. 枸杞的综合开发与利用[J]. 食品研究与开发,2012, 33(2): 223-227.
[9] 何进. 地骨皮成分研究进展[J]. 国外医药(植物药分册), 1996(2): 63-65.
[10] IMAI S, MURATA T, FUJIOKA S, et al.Isolation of beta-sitosterol-beta-dglucoside from the leaves of Lycium chinense mill[J]. Yakugaku zasshi, 1963, 83: 1092.
[11] 谢忱, 徐丽珍, 杨小江. 枸杞属植物化学成分研究进展[J]. 国外医学(中医中药分册), 1994(1): 9-13.
[12] FUKUDA T, YOKOYAMA J, OHASHI H.Phylogeny and biogeography of the genus Lycium (Solanaceae): Inferences from chloroplast DNA sequences[J]. Molecular phylogenetics and evolution, 2001, 19(2):246-58.
[13] ASANO N, KATO A, MIYAUCHI M, et al.Specific α-galactosidase inhibitors, N-methylcalystegines-structure/activity relationships of calystegines from Lycium chinense[J]. Eur J Biochem, 1997, 248(2): 296-303.
[14] HARSH M L.Tropane alkaloids from Lycium barbarum linn., in vivo and in vitro[J]. Current science, 1989, 58(14): 817-818.
[15] 雷建武, 米佳, 罗青, 等. 枸杞中类胡萝卜素及体外抗氧化活性研究[J]. 食品工业, 2015, 36(12): 5-8.
[16] HAN S H, LEE H H, LEE I S, et al.A new phenolic amide from Lycium chinense Miller[J]. Arch Pharm Res, 2002, 25(4): 433-437.
[17] LEE D G, PARK Y, KIM M R, et al.Anti-fungal effects of phenolic amides isolated from the root bark of Lycium chinense[J]. Biotechnol Lett, 2004, 26(14): 1125-1130.
[18] 孟令杰, 刘百联, 张英, 等. 地骨皮化学成分研究[J]. 中草药, 2014, 45(15): 2139-2142.
[19] FORINO M, TARTAGLIONE L, DELL'AVERSANO C, et al. NMR-based identification of the phenolic profile of fruits of Lycium barbarum (goji berries). Isolation and structural determination of a novel N-feruloyl tyramine dimer as the most abundant antioxidant polyphenol of goji berries[J]. Food chemistry, 2016, 194: 1254-1259.
[20] XIE L W,ATANASOV A G, GUO D A, et al.Activity-guided isolation of NF-κB inhibitors and PPARγ agonists from the root bark of Lycium chinense Miller[J]. J Ethnopharmacol, 2014, 152(3): 470-477.
[21] 邹澄, 赵庆, 陈昌祥. 枸杞酰胺甲的结构[J]. 云南植物研究, 1999(3): 112-114.
[22] SHINJI F,KOZUE Y,CHOHACHI K,et al.Structure of kukoamine A, a hypotensive principle of Lycium chinense root barks1[J]. Pergamon, 1980, 21(14):1355-1356.
[23] FUNAYAMA S,ZHANG G-R,NOZOE S.Kukoamine B, a spermine alkaloid from Lycium chinense[J].Phytochemistry,1995, 38(6):1529-1531.
[24] GAO K,MA D,CHENG Y, et al.Three new dimers and two monomers of phenolic amides from the fruits of Lycium barbarum and their antioxidant activities[J]. J agric food chem, 2015, 63(4): 1067-1075.
[25] YANG Y, AN Y, WANG W, et al.Nine compounds from the root bark of Lycium chinense and their anti-inflammatory activities[J]. Acta pharmaceutica sinica b, 2017, 7(4): 491-495.
[26] CHEN H, LI Y J, SUN Y J, et al.Lignanamides with potent antihyperlipidemic activities from the root bark of Lycium chinense[J]. Fitoterapia, 2017, 122: 119-125.
[27] CHIN Y W, LIM S W, KIM S H, et al.Hepatoprotective pyrrole derivatives of Lycium chinense fruits[J]. Bioorg Med Chem Lett, 2003, 13(1): 79-81.
[28] YOUN U J, KIL Y S, NAM J W, et al.New pyrrole alkaloids with bulky N‐alkyl side chains containing stereogenic centers from Lycium chinense[J]. Helvetica chimica acta,2013,96(8):1482-1487.
[29] HAN B H, PARK J H, PARK M H, et al.Studies on the alkaloidal components of the fruits of Lycium chinense[J]. Archives of pharmacal research, 1985, 8(4):249-252.
[30] 徐秀珍, 齐喜红. 薄层扫描法测定枸杞子中甜菜碱的含量[J]. 中成药, 2005(8): 941-943.
[31] 谢忱, 徐丽珍, 李宪铭, 等. 枸杞子化学成分的研究[J]. 中国中药杂志, 2001(5): 35-36.
[32] INBARAJ B S, LU H, HUNG C F, et al.Determination of carotenoids and their esters in fruits of Lycium barbarum Linnaeus by HPLC-DAD-APCI-MS[J]. J Pharm Biomed Anal, 2008, 47(4-5): 812-818.
[33] CHEN G, HUO Y, TAN D X, et al.Melatonin in Chinese medicinal herbs[J]. Life sciences, 2003, 73(1):19-26.
[34] 高凯. 宁夏枸杞子的活性成分研究和应用开发[D]. 西安:第四军医大学, 2014.
[35] KUN W, TATSUNORI S, WEI L, et al.Two novel steroidal alkaloid glycosides from the seeds of Lycium barbarum[J]. Chemistry & biodiversity, 2011, 8(12):2277-2284.
[36] GAO Z, ALI Z, KHAN I A.Glycerogalactolipids from the fruit of Lycium barbarum[J]. Phytochemistry,2008,69(16): 2856-2861.
[37] JUNG K, CHIN Y-W, KIM Y C, et al.Potentially hepatoprotective glycolipid constituents of Lycium chinense fruits[J]. Archives of pharmacal research, 2005, 28(12):1381-1385.
[38] LE K, CHIU F, NG K.Identification and quantification of antioxidants in Fructus lycii[J]. Food chemistry,2006,105(1):301-308.
[39] YUAN Y, WANG Y B, JIANG Y, et al.Structure identification of a polysaccharide purified from Lycium barbarium fruit[J]. Int J Biol Macromol, 2016, 82: 696-701.
[40] 魏秀丽,梁敬钰. 地骨皮的化学成分研究[J]. 中草药,2003, 34(7):7-8.
[41] PENG X, TIAN G.Structural characterization of the glycan part of glycoconjugate LbGp2 from Lycium barbarum L[J]. Carbohydr Res, 2001, 331(1): 95-99.
[42] 邹耀洪. 枸杞叶的黄酮类化学成分[J]. 分析测试学报,2002(1):76-78.
[43] 李行诺, 楚楚, 毛慧琼, 等. 地骨皮化学成分研究[J]. 中国现代应用药学, 2012, 29(9): 805-808.
[44] 魏秀丽, 梁敬钰. 地骨皮化学成分的研究[J]. 中国药科大学学报, 2002(4): 9-11.
[45] 周兴旺, 徐国钧, 王强. 地骨皮化学成分的研究[J]. 中国中药杂志, 1996(11): 35-36,64.
[46] INBARAJ B S, LU H, KAO T H, et al.Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS[J].J Pharm Biomed Anal,2010,51(3):549-556.
[47] YAO X,PENG Y,XU L J,et al.Phytochemical and biological studies of Lycium medicinal plants[J]. Chem Biodivers, 2011, 8(6): 976-1010.
[48] JIE Z, CHENXU D, LIANGSHENG W, et al.Anthocyanins composition and antioxidant activity of wild Lycium ruthenicum Murr. from Qinghai-Tibet Plateau[J]. Food chemistry, 2011, 126(3):859-865.
[49] 陈绥清, 王强, 龚孙莲, 等. 中药枸杞中的氨基酸分析[J]. 中国药科大学学报, 1991(1): 53-55.
[50] 王杰. 济南枸杞子化学成分分析[J].中国药学杂志,1991(5):269-270,314.
[51] GÜNDÜZ E, DURSUN R, ZENGIN Y, et al. Lycium barbarum extract provides effective protection against paracetamol-induced acute hepatotoxicity in rats[J]. Int J Clin Exp Med, 2015, 8(5): 7898-905.
[52] AHN M, PARK J S, CHAE S, et al.Hepatoprotective effects of Lycium chinense Miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats[J]. Acta histochemica,2014,116(6):1104-1112.
[53] XIAO J, LIONG E C, CHING Y P, et al.Lycium barbarum polysaccharides protect rat liver from non-alcoholic steatohepatitis-induced injury[J]. Nutr diabetes, 2013, 3(7): e81.
[54] 赵海鹰, 刘敏科, 李孝忠, 等. 枸杞对X线辐射致大鼠肾脏氧化损伤的防护作用[J]. 中国现代应用药学,2019,36(18): 2262-2266.
[55] 刘波, 谭雅琴, 丁协刚, 等. 枸杞多糖促进大鼠海绵体神经损伤修复的实验研究[J]. 中华男科学杂志,2017,23(11): 1043-1046.
[56] 黄晓兰, 杨明亮, 吴晓旻, 等. 枸杞多糖对雄性大鼠睾丸组织损伤的保护作用[J]. 中国公共卫生, 2003(9): 34-36.
[57] WU Q, LIU L T, WANG X Y, et al.Lycium barbarum polysaccharides attenuate kidney injury in septic rats by regulating Keap1-Nrf2/ARE pathway[J]. Life sciences, 2020, 242: 117240.
[58] SONG M K, ROUFOGALIS B D, HUANG T H.Reversal of the caspase-dependent apoptotic cytotoxicity pathway by taurine from Lycium barbarum (Goji Berry) in human retinal pigment epithelial cells: Potential benefit in diabetic retinopathy[J]. Evid Based Complement Alternat Med, 2012, 1: 323784.
[59] 黄洁. 枸杞多糖对光诱导的人视网膜色素上皮细胞氧化应激损伤及PI3K/Akt/mTOR信号通路的影响[D]. 银川:宁夏医科大学, 2020.
[60] SONG M K, SALAM N K, ROUFOGALIS B D, et al.Lycium barbarum (Goji Berry) extracts and its taurine component inhibit PPAR-γ-dependent gene transcription in human retinal pigment epithelial cells:Possible implications for diabetic retinopathy treatment[J]. Biochem Pharmacol, 2011, 82(9): 1209-1218.
[61] 杨薛康, 海春旭, 梁欣, 等. 枸杞提取物的抗氧化作用[J]. 第四军医大学学报, 2007(6): 518-520.
[62] 张丽, 李灵芝, 柴煊, 等. 枸杞子乙酸乙酯提取部位的抗氧化活性研究[J]. 武警医学院学报, 2008(4): 267-269.
[63] RAN Y,XIAO-MIN L,QI D.A study of Lycium barbarum polysaccharides (LBP) extraction technology and its anti-aging effect[J]. African journal of traditional, complementary, and alternative medicines,AJTCAM, 2013, 10(4):171-174.
[64] 郑慧丽, 曹程, 王颖异, 等. 基于“成分-靶点-通路”的枸杞子调控年龄相关性黄斑变性作用机制研究[J]. 中草药, 2021, 52(4): 1039-1048.
[65] 李晶,欧芹,孙洁. 枸杞多糖对衰老大鼠蛋白质氧化损伤影响的实验研究[J]. 中国老年学杂志,2007(24):2384-2385.
[66] 雷蕾, 袁国强, 刘健锋, 等. 枸杞叶中总黄酮对人肝癌细胞HepG2增殖与凋亡的影响[J]. 宁夏大学学报(自然科学版), 2014, 35(3): 255-260.
[67] ZHANG M, TANG X, WANG F, et al.Characterization of Lycium barbarum polysaccharide and its effect on human hepatoma cells[J]. International journal of biological macromolecules, 2013, 61:270-275.
[68] 胡妮, 杜标炎, 谭宇蕙, 等. 从诱导树突状细胞成熟角度探讨枸杞多糖联合CXC趋化因子配体10抗癌作用机制[J]. 广州中医药大学学报, 2015, 32(4): 641-647.
[69] CHAN H C, CHANG R C C, IP A K C, et al. Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma[J]. Experimental neurology, 2006, 203(1):269-273.
[70] HO Y S, YU M S, LAI C S W, et al. Characterizing the neuroprotective effects of alkaline extract of Lycium barbarum on β-amyloid peptide neurotoxicity[J]. Brain research, 2007,1158:123-134.
[71] LIU J, JIANG X, ZHANG Q, et al.Neuroprotective effects of Kukoamine A against cerebral ischemia via antioxidant and inactivation of apoptosis pathway[J]. Neurochemistry international,2017, 107:191-197.
[72] LI Y Y, HU S, HUANG Y Q, et al.Preventing H₂O₂ -induced toxicity in primary cerebellar granule neurons via activating the PI3-K/Akt/GSK3β pathway by kukoamine from Lycii Cortex[J]. Journal of functional foods, 2015, 17:709-721.
[73] 苗珍花, 于建春, 苗永霸, 等. 枸杞叶及枸杞多糖对快速老化模型小鼠行为学的影响[J]. 宁夏医科大学学报, 2013, 35(2): 117-121,129.
[74] CHEN W, CHENG X, CHEN J, et al.Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats[J]. PLoS one, 2014, 9(2): e88076.
[75] CHEN H, OLATUNJI O J, ZHOU Y.Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice[J]. Journal of natural medicines, 2016, 70(3): 610-619.
[76] JIANG Y, YU L, WANG M H.N-trans feruloyltyramine inhibits LPS-induced NO and PGE 2 production in RAW 264.7 macrophages: Involvement of AP-1 and MAP kinase signalling pathways[J]. Chemico-biological interactions, 2015, 235:56-62.
[77] 卫琮玲, 石渊渊, 任艳彩, 等. 地骨皮的降血糖机制研究[J]. 中草药, 2005(7): 95-97.
[78] 杨新波,黄正明, 曹文斌, 等. 枸杞多糖对正常小鼠及四氧嘧啶致高血糖小鼠血糖的影响[J]. 人民军医药学专刊, 1998(1): 11-13.
[79] 杨涓, 魏智清, 陈炜. 枸杞叶降血糖作用的初步研究[J]. 四川中医, 2009, 27(4): 47-49.
[80] 尹长江, 杨坤宝, 张学军, 等. 枸杞总多糖对2型糖尿病大鼠的降糖作用研究[J]. 中成药, 2014, 36(8): 1750-1753.
[81] 黄云兰,梁耿,韦凯东.枸杞多糖对大鼠糖尿病的作用研究[J].中国实验方剂学杂志, 2012, 18(22): 275-278.
[82] LUO Q, CAI Y, YAN J, et al.Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum[J]. Life sciences, 2004, 76(2): 137-149.
[83] 徐曼艳, 张红锋, 王煜飞. 枸杞多糖对四氧嘧啶损伤的离体大鼠胰岛细胞的保护作用[J]. 中药材, 2002(9): 649-651.
[84] 王发选, 宋琦如, 杨正贵. 枸杞多糖对中波紫外线致人皮肤成纤维细胞损伤的保护作用[J]. 宁夏医学院学报, 2006(6): 501-503.
[85] 李红光,高银娟,韩喜莉,等. 枸杞多糖在UVB辐射人角质形成细胞氧化损伤中的保护作用[J]. 中国医学工程,2011,19(12):60-61.
[86] 李德远, 汤坚, 徐现波, 等. 枸杞多糖对慢性辐射小鼠细胞凋亡及bc1-2基因表达的影响[J]. 营养学报, 2005(3): 235-237.
[87] ZHAO Z,LUO Y, LI G, et al.Thoracic aorta vasoreactivity in rats under exhaustive exercise: Effects of Lycium barbarum polysaccharides supplementation[J]. J Int Soc Sports Nutr, 2013, 10(1): 47.
[88] 张文娟, 李光华, 朱玲勤, 等. 枸杞多糖在保护力竭运动大鼠胸主动脉血管内皮细胞损伤中的作用研究[J]. 时珍国医国药, 2017, 28(11): 2595-2598.
[89] 李雯,陈兰, 戚迪, 等. 枸杞多糖通过Akt/eNOS通路减轻LPS致ARDS小鼠肺损伤[J]. 中国药理学通报,2018,34(9):1258-1263.
[90] ZHANG M, LI F, POKHAREL S, et al.Lycium barbarum polysaccharide protects against Homocysteine-induced Vascular smooth muscle cell proliferation and phenotypic transformation via PI3K/Akt pathway[J]. J Mol Histol, 2020, 51(6): 629-637.
[91] 李炳龙, 齐永秀, 刘常丽, 等. 甜菜碱药理作用机制及其药效学研究进展[J].中国新药杂志,2008(18):1571-1574.
[92] 孔玉珊, 黄少兰, 谢明霞, 等.排风藤提取物中东莨菪苷对神经细胞的抗氧化活性研究[J].天然产物研究与开发,2014, 26(6):943-946.
[93] NOJIMA H, KIMURA I, CHEN FJ, et al.Antihyperglycemic effects of N-containing sugars from Xanthocercis zambesiaca, Morus bombycis, Aglaonema treubii, and Castanospermum australe in streptozotocin-diabetic mice[J]. J Nat Prod,1998,61(3):397-400.
[94] 张崇坚. 宁夏枸杞的综合开发利用研究[D].武汉:湖北工业大学,2015.