Study on seed dormancy and germination characteristics of Toxicodendron succedaneum

doi:10.14088/j.cnki.issn0439-8114.2023.07.017

Abstract

Abstract: In order to explore the dormancy causes, dormancy release methods and germination characteristics of Japanese wild lacquer（Toxicodendron succedaneum） seeds, mature Japanese wild lacquer seeds were used as materials to observe their morphological structure and water absorption, determine the effects of methanol extracts from different parts of Japanese wild lacquer seeds on the germination and growth of Chinese cabbage seeds, compare the effects of different dewaxing treatments combined with gibberellin soaking on the germination rate of Japanese wild lacquer seeds, and determine the changes of physiological and biochemical indexes during seed germination of Japanese wild lacquer. The results showed that, the seeds of Japanese wild lacquer tree had poor water permeability and physical dormancy. The water absorption rate was only 34.17% after soaking in clean water for 15 days, and the water absorption rate reached 77.12% after acid etching treatment and soaking in clean water for 5 days. The endotesta extract of Japanese wild lacquer had a significant inhibitory effect on the germination and growth of Chinese cabbage seeds. The endotesta contained endogenous substances that inhibited germination and had physiological dormancy. 98% H₂SO₄ dewaxing treatment for 20 min combined with soaking seeds in 300 mg/L gibberellin solution for 12 h could break the seed dormancy of Japanese wild lacquer tree, significantly improve the germination potential and germination rate compared with the control, and make the germination early and neat. The seed germination potential reached （54.33±0.58）% and the germination rate reached （97.67±2.52）%. The seeds started to germinate on the 3rd day and all had germinated on the 10th day. During seed germination, the contents of soluble sugar and soluble protein decreased significantly, the activity of POD and α-amylase increased significantly. The contents of ABA and GA₃ in the seeds of Japanese wild lacquer tree were higher and lower in the dormancy stage. The content of GA₃ increased sharply in the imbibition stage. After the radicle grew, the contents of ABA and GA₃ decreased significantly.

Key words: Toxicodendron succedaneum, seed dormancy, dormancy release, germination percentage, germination physiology

CLC Number:

CHEN Fa-zhi, TONG Jun, YANG Shou-kun, ZHAI Jing-hua, GAO Hui-jiang, LIU Zhong. Study on seed dormancy and germination characteristics of Toxicodendron succedaneum[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(7): 95-100.

References

[1] 方庆标,余林根,周云,等. 日本野漆树引种育苗初报[J]. 江西林业科技,1996(6):26-28.
[2] 陈新强,李志良,陈桂琼,等. 日本野漆树在梅州地区的引种表现[J]. 林业与环境科学, 2020, 36(4):41-46.
[3] 刘伟. 日本野漆树漆蜡溶剂萃取及理化特性的研究[D].长沙:中南林业科技大学,2008.
[4] 何承忠, 罗一然, 李旦, 等. 日本漆树高效快速扩繁方法[P]. 中国专利:2017100387183,2017-01-17.
[5] 舒志群,李相玺,左长清,等. 红壤水土流失区日本野漆树的开发利用[J].中国水土保持, 2000(3):32-33.
[6] 张宇思,姜洪芳,肖正春. 漆树籽植物资源及其开发利用[J]. 中国野生植物资源,2017, 36(6):1-4.
[7] LABAT E, MOREL M H, POUAU X.Effects of laccase and ferulic acid on wheat flour doughs[J]. Cereal chemistry, 2000, 77(6):883-894.
[8] 郑育桃,李林松,雷小林,等.日本黄栌栽培技术[J]. 经济林研究,2006, 24(4):41-45.
[9] 张飞龙,陈振峰,张武桥,等. 秦巴山区漆树资源可持续发展及规模化管理模式研究[J]]. 中国生漆,2004(l):1-27.
[10] 孔艳菊,孙明高,胡学俭,等. 干旱胁迫对黄栌幼苗几个生理指标的影响[J]. 中南林学院学报,2006,26(4):42-46.
[11] 雷小林,李猛,文娟,等. 日本野漆树嫁接繁育技术初探[J]. 经济林研究,2006,24(3):53-55.
[12] 杨静静. 野漆树轻型基质播种育苗技术研究[D].长沙:中南林业科技大学,2011.
[13] 袁雅琪,钟秋平,袁小军,等. 不同处理对3个种源漆树种子萌发的影响[J].经济林研究,2017,35(3):63-69.
[14] 杨锦芬,刘艳,何瑞,等. 阳春砂不同栽培品种种子的比较研究[J]. 广州中医药大学学报, 2011, 28(1):66-69.
[15] 焦喜来,程伟.文冠果种子浸提液对白菜种子萌发的影响[J].中国林副特产,2012(3):17-20.
[16] 童俊, 周媛, 董艳芳, 等. 一种提高日本野漆树种子发芽率以及快速催芽的方法[P]. 中国专利:2018109325098,2018-08-14.
[17] 杨佳欢. 紫叶加拿大紫荆种子的休眠与萌发特性[D]. 江苏扬州:扬州大学,2021.
[18] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社,2000.
[19] 王翠玲,杨晓明. 不同豌豆品种淀粉酶活性的差异分析[J]. 实验室科学,2019, 22(4):46-49.
[20] 张志良,瞿伟菁,李小芳. 植物生理学实验指导[M]. 第3版. 北京:高等教育出版社,2009.
[21] 庆军,朱利利,杜红岩. 杜仲雌雄花发育与内源激素含量动态变化的关系[J]. 中南林业科技大学学报,2021,41(4):58-65.
[22] BASKIN C C, BASKIN J M.Seed: Ecology, biogeography, and evolution of dormancy and germination[M]. 2nd. San Diego,USA: Academic press,1989.
[23] 孙婷. 栝楼(Trichosanthes kirilowii Maxim)种子的休眠特性及促萌措施研究[D].杭州:浙江师范大学,2010.
[24] 刘慧娜,张克亮,赵大球,等. 种子休眠与萌发综述[J].分子植物育种,2020, 18(2):621-627.
[25] 杨江梅. 漆树育苗和造林技术研究[J]. 绿色科技,2017(3):142-143.
[26] 唐丽,张瑜,傅超凡,等. 不同化学药剂处理对野漆树种子发芽率的影响[J]. 广东农业科学,2011(10):23-25.
[27] 褚泽龙. 玉铃花种子休眠与萌发特性研究[D].山东泰安:山东农业大学,2015.
[28] FINCH-SAVAGE W E,LEUBNER-METZGER G. Seed dormancy and the control of germination[J]. New phytologist,2006,171(3):501-523.
[29] 郑安俭,王州飞,张红生.作物种子萌发生理与遗传研究进展[J].江苏农业学报,2017,33(1):218-223.
[30] 马诗雨,杜娟,李叶芳,等. 滇南杜鹃种子萌发特性研究[J].种子,2019, 38(9):73-76, 80.
[31] 张谦. 天目琼花种子萌发生理生化特性研究[D].山东泰安:山东农业大学,2019.
[32] 苏海兰, 周先治, 李希, 等. 七叶一枝花种子萌发不同阶段生理生化变化研究[J]. 中草药, 2017, 48(22): 4755-4763.
[33] ALI-RACHEDI S, BOUINOT D, WAGNER M H, et al.Changes in endogenous abscisic acid levels during dormancy release and maintenance of mature seeds: Studies with the Cape Verde Islands ecotype, the dormant model of Arabidopsis thaliana[J]. Planta, 2004, 219:479-488.