Determination of prohibited pesticide residues in white peony by liquid chromatography-tandem mass spectrometry

doi:10.14088/j.cnki.issn0439-8114.2023.07.023

Abstract

Abstract: A liquid chromatography-tandem mass spectrometry （LC-MS/MS） method was established for the simultaneous determination of 29 prohibited pesticide residues in the white peony. The white peony powder sample was soaked overnight in acetonitrile and extracted by ultrasound. The solid phase extraction column was used for purification, and 29 prohibited pesticide residues were analyzed by LC-MS/MS positive ion mode scanning. The results showed that the linear correlation coefficient of 29 prohibited pesticides was good （R²>0.995 1）, with a detection limit of 0.000 1~0.030 0 mg/kg and a quantitative limit of 0.000 3~0.090 0 mg/kg; at the spiked level of 0.01~0.50 mg/kg, the recovery rates of 29 prohibited pesticides were 66.8%~108.4%, and the relative standard deviation （n=6） was 0.9%~12.3%. This method had been successfully applied to the qualitative and quantitative analysis of 29 prohibited pesticides in the quality control samples of the white peony. The qualitative accuracy was accurate, and the quantitative results were within the uncertainty range of the quality control certificate. It was suitable for the residual analysis of 29 prohibited pesticides in the white peony.

Key words: liquid chromatography-tandem mass spectrometry （LC-MS/MS）, white peony, Patagonia lactiflora Pall., prohibited pesticide residues, matrix effect

CLC Number:

TS657.7

LUO Fa-mei, MAO Jing-chun, HUANG Ying-rui, ZHOU Qin, HE Xi, CHENG Long. Determination of prohibited pesticide residues in white peony by liquid chromatography-tandem mass spectrometry[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(7): 130-136.

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