Characteristics of microbial quantity and enzyme activity in tobacco planting soil under application of mixed seeding green manure

doi:10.14088/j.cnki.issn0439-8114.2024.02.016

Abstract

Abstract: In order to study effects of the mixed seeding green manure application on the microbial count and enzyme activity of tobacco planting soil, a site-specific field experiment was conducted by performing no green manure（control）, Lolium perenne monocropping, Vicia villosa var. glabrescens monocropping, and Lolium perenne and Vicia villosa var. glabrescens mixture （application of mixed seeding green manure）. The microbial quantity and enzyme activities of soil were analyzed after the consecutive 2-year test. The results indicated that the total number of bacteria and nitrifying bacteria in soil under application of mixed green manure were 2.25~3.62 times and 1.60~3.11 times as the control, 1.31~1.76 times and 1.43~2.92 times as Lolium perenne monocropping, 1.25~1.88 times and 1.08~1.27 times as Vicia villosa var. glabrescens monocropping. The numbers of actinomyces and fungi in soil under application of mixed green manure were 1.13~2.00 times and 1.02~1.93 times as green manure monocropping at the early stage of the tobacco planting field, respectively, and lower than those of the green manure monocropping at the later stage of the tobacco planting field. There was no significant difference in the number of ammonifying bacteria in the soil among the treatments at the middle and later stage of the tobacco planting field. The activities of soil catalase, sucrase, urease, acid phosphatase and cellulase in the soil under application of the mixed green manure at different growth stages were significantly higher than those of the control, and also higher than those of Lolium perenne monocropping and Vicia villosa var. glabrescens monocropping. It could be seen that mixed seeding green manure application was beneficial to improve the micro-ecological environment of tobacco planting soil and could be used as a technical model for tobacco planting soil conservation.

Key words: tobacco-planting soil, mixed seeding green manure, soil microorganism, soil enzyme activity

CLC Number:

CHEN Wei, CHEN Yi, JIANG Chao-ying, YANG Quan-liu, LU Xian-ren, JIANG Wei. Characteristics of microbial quantity and enzyme activity in tobacco planting soil under application of mixed seeding green manure[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(2): 88-93.

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