Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents

doi:10.14088/j.cnki.issn0439-8114.2024.09.016

Abstract

Abstract: The pathogens from Sophora tonkinensis Radix were isolated by conventional tissue separation method, the pathogenicity was determined by Koch's formula, and the pathogens were identified based on morphological characteristics, mycelium fusion group determination, nuclear fluorescence staining and rDNA-ITS sequence analysis. The inhibitory activities of 5 fungicides against pathogens were measured by the mycelium growth rate method. The results showed that the pathogen was Rhizoctonia solani, belonging to the fusion group AG-1. 98% hymexazol soluble power （SP）, 75% oxime ·pentazolol water dispersible granule （WG）, 250 g/L pyraclostrobin emulsifiable concentrate （EC）, 1 billion /g Trichoderma harziana suspension concentrate （SC）, and 50% carbendazim wettable power （WP） all had good inhibitory effects on the growth of pathogenic fungi mycelia. The inhibitory medium effective concentrations （EC₅₀） were 0.061 1, 0.575 7, 0.074 8, 0.284 3, 0.740 5 mg/L, respectively.

Key words: Sophora tonkinensis Radix, sclerotinia, identification of pathogen, screening of laboratory agents, Rhizoctonia solani

CLC Number:

SONG Li-sha, JIANG Ni, ZHANG Zhan-jiang, WEI Shu-gen, QIU Zhuo-qiu, HUANG Qi, ZHAN Xin-jie, PAN Li-mei. Identification of pathogen of sclerotinia on Sophora tonkinensis Radix and screening of laboratory agents[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 89-94.

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