HUBEI AGRICULTURAL SCIENCES ›› 2024, Vol. 63 ›› Issue (12): 67-72.doi: 10.14088/j.cnki.issn0439-8114.2024.12.012

• Plant Protection • Previous Articles     Next Articles

Screening and control effect of highly effective antagonistic bacteria against cotton verticillium wilt

ZHANG De-zheng1, NIE Tai-li1, YANG Jun2, KE Xing-sheng1, YAN De-peng1, NIE Zhang-qing3, ZHONG Min1   

  1. 1. Cash Crops Research Institute of Jiangxi Province, Nanchang 330000, China;
    2. Jiangxi Jiangmian Technology Development Co.,Ltd., Jiujiang 332000,Jiangxi, China;
    3. Jiangxi Agricultural Technology Promotion Center, Nanchang 330000, China
  • Received:2023-11-21 Online:2024-12-25 Published:2025-01-08

Abstract: Using Verticillium dahliae Kleb as indicator bacteria, the biocontrol strains with high activity were screened by confronation culture and metabolite inhibition test. The preventive effect of the tested strain was determined by pot experiment. The results showed that 162 strains of bacteria with biocontrol effect were screened from more than 1 000 strains isolated from cotton rhizosphere soil, and 4 strains with good comprehensive effect were obtained by using the plate face-off method. Strains XJ5-6 and XJ6-96 showed better inhibitory effects, with the band width exceeding 5.0 mm and the inhibitory rate reaching 67.54% and 66.86%, respectively. The results of pot experiment showed that XJ5-6 and XJ6-96 combined inoculation had a good control effect on verticillium wilt of cotton, and the control effect was 32.2% and 29.9% on 28 and 40 days, respectively. XJ6-96 could improve the germination rate of cotton seed.

Key words: cotton verticillium wilt, rhizosphere bacteria, highly effective antagonistic bacteria, antagonistic activity

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