Rapid detection of Pseudomonas aeruginosa in packaged drinking water using glycosylation functionalized magnetic nanoparticle materials enrichment fluorescence method

doi:10.14088/j.cnki.issn0439-8114.2025.02.027

Abstract

Abstract: Based on the specific recognition of glycosylation functionalized magnetic nanoparticle materials by Pseudomonas aeruginosa and the secretion of gelatinase by Pseudomonas aeruginosa, the release of FITC was induced from load GNP@FITC functionalized nanofiber membrane. The fluorescence intensity of functionalized nanofiber membrane was measured using a fluorescence spectrophotometer to establish a rapid detection method for Pseudomonas aeruginosa in packaged drinking water. This method used glycosylation functionalized magnetic nanoparticle materials to enrich Pseudomonas aeruginosa. The amount of glycosylation functionalized magnetic nanoparticle materials added to 250 mL of drinking water was 5.00 mg, and the optimal co culture time between glycosylation functionalized magnetic nanoparticle materials and Pseudomonas aeruginosa was 6 hours. The optimal co-culture time between load GNP@FITC functionalized nanofiber membrane and Pseudomonas aeruginosa was 30 minutes, with a test time of less than 8 hours. The detection limit of the method was 0.004 CFU/mL, indicating strong specificity. Compared with the testing method in GB 8538—2022 ‘National Food Safety Standard for Drinking Natural Mineral Water Inspection Method’, this method shortened the testing time and the detection limit could meet regulatory needs, making it a useful supplement to traditional testing methods.

Key words: Pseudomonas aeruginosa, glycosylation functionalization, magnetic nanoparticle materials, enrichment, fluorescence, rapid detection, packaged drinking water

CLC Number:

TS207.3

HUANG Hui, GUO Yin-li, PENG Qing-zhi, LU Zhen-tan, WANG Fu-yan, LIU Di, ZHANG Miao. Rapid detection of Pseudomonas aeruginosa in packaged drinking water using glycosylation functionalized magnetic nanoparticle materials enrichment fluorescence method[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 171-178.

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