Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system

doi:10.14088/j.cnki.issn0439-8114.2020.17.013

Abstract

Abstract: Taking nine soil samples growing Cardamine violifolia O. E Schulz from three area （Shuanghe town, Enshi city; Wufeng county, Yichang city, Hubei province and Shimen county, Changde city, Hunan province） as materials, the microbial community structure and diversity were analyzed by using Illumina MiSeq high-throughput sequencing system. The results showed that the microbial community composition of S1 soil sample form Shuanghe and S2 soil sample from Wufeng were similar, but the composition were quite different between S1 soil sample and S3 soil sample from Shimen, and were different between S2 and S3 soil sample. The diversity index and rarefaction curves analysis showed that in nine soil samples, the highest microbial diversity was No. S1.2 soil sample from Shuanghe and the lowest microbial diversity was No. S3.2 soil sample from Shimen. It indicated that the community diversity of Shuanghe soil sample was the highest and that of Shimen soil sample was the lowest. Proteobacteria was the dominant population in three area soil. The same phyla of the three area were Crenarchaeota, Acidobacteria, Actinobacteria, Bacteroidetes, Chlamydiae, Chloroflexi, Firmicutes, Gemmatimonadetes, Nitrospirae, OD1, Planctomycetes, Proteobacteria, Verrucomicrobia and WS3.

Key words: Cardamine violifolia O. E Schulz, Illumina MiSeq high-throughput sequencing, rizosphere soil, microbial diversity

CLC Number:

Q948.15
Q938

CHEN Fei-fei, CONG Xing, XIANG Ji-qian, LI Hong-ying, SUN Ju-zhi, XU Feng, XUE Hua. Rhizosphere soil microorganisms diversity of Cardamine violifolia O. E Schulz analyzed by Illumina MiSeq high-throughput sequencing system[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(17): 58-62.

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