Construction of the protein-protein interaction network of Chlamydia abortus

doi:10.14088/j.cnki.issn0439-8114.2021.22.026

HUBEI AGRICULTURAL SCIENCES ›› 2021, Vol. 60 ›› Issue (22): 124-127.doi: 10.14088/j.cnki.issn0439-8114.2021.22.026

• Animal Husbandry & Veterinary Medicine • Previous Articles Next Articles

Construction of the protein-protein interaction network of Chlamydia abortus

LIU Wei, YUAN Fang-yan, ZHOU Dan-na, LIU Ze-wen, GAO Ting, GUO Rui, YANG Ke-li, DUAN Zheng-ying, LIANG Wan, TIAN Yong-xiang

Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Hubei Science-observation Experiment Station of Veterinary Medicine and Diagnostic Technology （Ministry of Agriculture and Rural Affairs）,Wuhan 430064,China

Received:2021-04-25 Online:2021-11-25 Published:2021-12-10

Abstract

Abstract: Analysis of the pathogen interactome is a powerfull approach for dissecting potential signal trasducion and new drug targets. A comprehensive protein-protein interaction network for Chlamydia abortus consisting of 220 proteins and 1 276 interaction pairs was constructed. Our analysis unraveled 20 proteins with highest interactions in the network, most of them are related to the function of transcription, translation and molecular chaperone. Further analysis showed that the chaperone proteins DnaK, GroEL and DnaJ had a high frequency of interaction, and could interact with multiple functional proteins such as ribosomal proteins and metabolism-related proteins. The results suggested that if the function of DnaK, GroEL and DnaJ were inhibited, the normal life activities of Chlamydia abortus would be greatly affected. Taken together, this study therefore provides valuable clues in exploring new signaling protein and new drug targets.

Key words: Chlamydia abortus, protein interaction, interaction network

CLC Number:

S852.67

LIU Wei, YUAN Fang-yan, ZHOU Dan-na, LIU Ze-wen, GAO Ting, GUO Rui, YANG Ke-li, DUAN Zheng-ying, LIANG Wan, TIAN Yong-xiang. Construction of the protein-protein interaction network of Chlamydia abortus[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(22): 124-127.

References

[1] SIARKOU V, LAMBROPOULOS A F, CHRISAFI S, et al.Subspecies variation in greek strains of Chlamydophila abortus[J]. Vet Microbiol, 2002,85: 145-157.
[2] SETH-SMITH H M B, BUSÓ L S, LIVINGSTONE M, et al. European Chlamydia abortus livestock isolate genomes reveal unusual stability and limited diversity, reflected in geographical signatures[J]. BMC genomics, 2017, 18(1):344.
[3] NICOLAS P, LAURE G, KARINE L, et al.Chlamydia abortus in pregnant woman with acute respiratory[J]. Distress syndrome, 2020, 26(3): 628-629.
[4] OPOTA O, BROUILLET R, GREUB G, et al.Methods for real-time PCR-based diagnosis of Chlamydia pneumoniae, Chlamydia psittaci, and Chlamydia abortus infections in an opened molecular diagnostic platform[J]. Methods Mol Biol, 2017, 1616:171-181.
[5] CHEONG H C, LEE C Y Q, CHEOK Y Y, et al. Chlamydiaceae:Diseases in primary hosts and zoonosis[J]. Microorganisms, 2019, 7:146.
[6] WALDER G, MEUSBURGER H, HOTZEL H, et al.Chlamydophila abortus pelvic inflammatory disease[J]. Emerg Infect Dis, 2003, 9:1642-1644.
[7] 吴移谋. 衣原体[M]. 北京:人民卫生出版社,2012.
[8] 张志君,周继章. 流产衣原体研究进展[J]. 动物医学进展,2017,38(4):102-107.
[9] CUI T, ZHANG L, WANG Z X, et al.Uncovering new signaling proteins and potential drug targets through the interactome analysis of Mycobacterium tuberculosis[J]. BMC genomics, 2009, 10: 118.
[10] ALTSCHUL S F, MADDEN T L, SCHAFFER A A, et al.Gapped BLAST and PSI-BLAST:A new generation of protein database search programs[J]. Nucleic Acids Res,1997, 25: 3389-3402.
[11] SHANNON P, MARKIEL A, OZIER O, et al.Cytoscape:A software environment for integrated models of biomolecular interaction networks[J]. Genome Res, 2003, 13:2498-504.
[12] 杨金莹,孙爱清,刘箭,等.热激蛋白ClpB的结构和功能[J].植物生理学通讯,2006,42(2):326-330.
[13] SEGURA-PENA D, SEKULIC N, ORT S.Substrate-induced conformational changes in human UMP/CMP kinase[J]. J Biol Chem, 2004, 279(32):33882-33889.
[14] ZHAXYBAYEVA O, GOGARTEN J P. Bootstrap, Bayesian probability and maximum likelihood mapping:Exploring new tools for comparative genome analyses[J]. BMC genomics, 2002, https://doi.org/10.1186/1471-2164-3-4.

Construction of the protein-protein interaction network of Chlamydia abortus

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	LU Yan-yan, WANG Chao, SHI Fu-mei. Research advances on protein interaction technology [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 5-10.
[2]	XIAO Hong-wei, HUA Zai-dong, BI Yan-zhen, REN Hong-yan, HUA Wen-jun, ZHANG Li-ping, ZHU Zhe, LIU Xi-mei, ZHENG Xin-min. Analysis of Protein Interaction in Sow Reproduction [J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(23): 153-162.