Study on the Aseptic Culture of Hybridized Offspring of Rhododendron

doi:10.14088/j.cnki.issn0439-8114.2018.22.025

Abstract

Abstract: Taking Rhododendron cultivar "Rhododendron obtusum‘Yanzhimi’×Rhododendron hybridum‘Chunwubao’" as experimental materials, the effects of different methods of disinfection, initial culture of different kinds of media,different plant growth regulators, low temperature treatment and light conditions on seed germination and the growth were studied, and the effects of 8 kinds of 1/2 WPM medium supplemented with different concentrations of plant growth regulators on the rooting rate of hybrid sterile seedlings were also studied. The results showed that the optimum disinfection method for Rhododendron hybrid seeds was 1.5% NaClO solution for 10 min and 75% ethanol for 30 s. On the condition of 20 days low-temperature treatments at 4 ℃,and culturing Rhododendron hybrid seed with 100 mg/L GA₃ in 1/2 WPM medium, the highest germination rate was got,and the value was 56.7%. Light irradiation promoted the germination of Rhododendron hybrid seed,the germination rate of seed under light treatment was higher than that of dark treatment,and the seed germination time was also 3~7 days earlier. On the condition of 1/2 WPM medium with 0.5 mg/L NAA and 0.1 mg/L IBA,Rhododendron hybrid sterile seedling had the highest rooting rate.

Key words: Rhododendron, low temperature, aseptic sowing, germination rate, rooting rate, cross-incompatibility

CLC Number:

S681.9

ZI Hong, YANG Jing-yu, FANG Lin-chuan, ZHOU Yuan. Study on the Aseptic Culture of Hybridized Offspring of Rhododendron[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(22): 90-95.

References

[1] 高文强,樊金会,赵红霞,等.杜鹃花组培快繁技术的研究[J].山东林业科技,2011,41(5):47-49.
[2] 苗永美,简兴.杜鹃组培的研究[J].北方园艺,2004,28(3):76-77.
[3] 张茂林,樊金会.杜鹃花种子休眠打破技术的研究[J].山东林业科技,2011,41(1):19-22.
[4] 沈羊城,侯金艳,刘文博,等.山桐子种子快速高效萌发研究[J].中国农学通报,2015,31(13):5-9.
[5] 鲁黎明,安影.不同消毒剂对烟草种子消毒效果及萌发的影响[J].种子,2012,31(4):93-95.
[6] WILBURR C,ANDERSON A.Revised tissue medium for shoot multiplication of rhododendro[J].J Amer Soc Hort Science,1984, 109(3):343-347.
[7] ECONOMOU A S,READ P E.In vitro shoot proliferation of minnesota deciduous azalea[J].Hort Science,1984,9(1):60-61.
[8] 杨乃博. 春夏鹃的试管繁殖[J].植物生理学通讯,1985(5):38-39.
[9] LLOYD G B,MC COWN B H. Commercially feasiboe micropropagation of mountain laurel,Kalmia latifolia,by use of shoot-tip culture[J].Combined Proceedings,International Plant Propagators’ Society,1980,30:421-427.
[10] 邓百万,陈文强,高菲菲.比利时杜鹃的组织培养研究[J].氨基酸和生物资源,2002(3):25-27.
[11] 宗宪春,李振山,孙美华.二种杜鹃花快速繁殖的研究[J].牡丹江师范学院学报(自然科学版),1999(1):5.
[12] 郑茜子,袁柳祥,赵冰,等.秦岭美容杜鹃种子组培快繁技术研究[J].北方园艺,2015,39(24):75-79.
[13] 罗琳,白洁,陈超,等.美容杜鹃叶片再生及愈伤组织耐热生理研究[J].西北植物学报,2014,34(7):1377-1382.
[14] 莫云容,张培欣,邵贵芳,等.不同浓度赤霉素和双氧水对刺茄种子萌发的影响[J].北方园艺,2016,40(9):32-34.
[15] 金小平,尹瑞.GA₃对榛子种子萌发的影响[J].北方果树,2011(4):14-15.
[16] 潘学军,张文娥,樊卫国.外源激素处理对贵州毛葡萄种子发芽的影响[J].种子,2007(1):25-27.
[17] 付婷婷,程红焱,宋松泉.种子休眠的研究进展[J].植物学报,2009,44(5):629-641.
[18] 王伟青,程红焱.拟南芥突变体种子休眠与萌发的研究进展[J].植物学通报,2006,23(6):625-633.
[19] 陈庆亮,王华磊,王志芬,等.低温层积与外源GA₃对肉苁蓉种子萌发及其内源GA和ABA含量的影响[J].植物生理学通讯, 2009,45(3):270-272.
[20] 于进英,黄青,李晓鹏,等.GA₃、低温冷藏和光照对真薰衣草种子萌发与幼苗生长的影响[J].种子,2012,31(5):81-84.
[21] 马丽妍,董春兰,言燕华,等.低温和GA₃凤丹种子萌发及碳氮代谢物和内源激素含量的影响[J].植物资源与环境学报,2014, 23(4):40-46.
[22] 于玲,钟原,王莹,等.低温和赤霉素对紫斑牡丹种子萌发和幼苗生长的影响[J].北京林业大学学报,2015,37(4):120-126.
[23] 侯冬花,呼凤兰,赵彦华.低温层积与GA₃综合处理促进野生杏种子萌发初探[J].山西果树,2012(6):16,23.