Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature

doi:10.14088/j.cnki.issn0439-8114.2020.24.038

Abstract

Abstract: CN01 and SJB016 (a low temperature resistant cotton) were used as materials to study the effects of low temperature treatment on cotton in the two-leaf period. Firstly, We separately measured the contents of malondialdehyde (MDA), soluble sugar (SS) and proline (Pro) in CN01 and SJB016 at 25 ℃ (CK) and 12 ℃ (low temperature treatment) for 0, 3, 6, 12, 24, 48 and 72 h. It was found that the contents of MDA, SS, and Pro in CN01 and SJB016 increased after 12 ℃ treatment compared with 25 ℃. After low temperature treatment, the content of MDA in SJB016 increased less than CN01, the content of SS in SJB016 increased larger than CN01, and the content of Pro in SJB016 increased less than CN01. Transcriptome analysis of cotton leaves in two-leaf period after low temperature treatment for 6 hours showed that the photosynthesis-related genes were up-regulated in CN01 after 12 ℃ treatment, while the genes of photosystem II oxygen-releasing complexes (PsdP, PsdQ, et al) were down-regulated in SJB016, compared with 25 ℃. The up-regulated differentially expressed genes associated with phytohormone were more than down-regulated genes in CN01 and SJB016, and ethylene-responsive transcription factors ERF053, ERF020, and ERF061 significantly up-regulated. These genes are deemed to play an important role in improving the cold resistance of cotton.

Key words: cotton leaf, physiological response, transcriptome, gene expression, low temperature treatment

CLC Number:

Q945.78

WANG Xiao-man, WANG Qiong-shan, WANG Xiao-gang, ZHANG Jiao-hai, ZHANG You-chang, ZHANG Xing-zhong, BIE Shu, XIA Song-bo. Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(24): 169-176.

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