Loading...

Table of Content

    25 December 2020, Volume 59 Issue 24
    Reviews
    Research progress on the biological activity and function of plant polysaccharides
    ZHANG Qin, LI Mei-dong, LUO Kai, HUANG Xiu-fang
    2020, 59(24):  5-8.  doi:10.14088/j.cnki.issn0439-8114.2020.24.001
    Asbtract ( 547 )   PDF (1522KB) ( 459 )  
    References | Related Articles | Metrics
    With the development of chemistry, nutrition, immunology and traditional Chinese medicine, people's research on plant polysaccharides continues to deepen. A number of fruitful research results have been obtained, providing a large amount of experimental data and theoretical basis for the study of human health and the biological activity of plant polysaccharides themselves. This paper was mainly to review the biological activity of plant polysaccharides in recent years, in order to provide reference for subsequent research.
    Research on the application of key information infrastructure security strategy
    XING Li-ping, CHEN Kan, WANG Fan
    2020, 59(24):  9-15.  doi:10.14088/j.cnki.issn0439-8114.2020.24.002
    Asbtract ( 434 )   PDF (3443KB) ( 213 )  
    References | Related Articles | Metrics
    On the basis of combing the security problems of key information infrastructure in Hubei province, combining the grade evaluation report to reflect the main problems and suggestions for rectification, and according to the research ideas of constructing security architecture, the technical strategies including strengthening online security product protection strategy, adding redundant network key nodes and seamless access of security products to the current network are adopted to carry out phased rectification step by step. The results show that the application of various techniques solves the problem of missing redundant design of WAN nodes in the key information infrastructure of Hubei province, and alleviates the irregular situation that the login address of core network equipment and server is not uniform, the password complexity and the periodic replacement are difficult to implement. Through the grade evaluation and risk assessment of the evaluation organization, the safety protection grade requirements and standards of the key information infrastructure in Hubei province are gradually related, and the safety protection ability is improved year by year.
    Research progress on the function of ubiquitination modification in DNA damage repair
    QIN Xiao-bing
    2020, 59(24):  16-20.  doi:10.14088/j.cnki.issn0439-8114.2020.24.003
    Asbtract ( 418 )   PDF (2767KB) ( 136 )  
    References | Related Articles | Metrics
    DNA damage can cause instability of genome, therefore, which makes the normal cells cancerous. Therefore, the DNA damage repair is essential for genome stability and preventing cells from becoming cancerous. There are numerous DNA damage repair factors participate in DNA damage repair response in the cells, some of them will be modificated when they functionating in DNA damage repair response, one of the most significant modifications is ubiquitination modification. In this review, we will shed light on the research progress of ubiquitination modification in the DNA damage repair response,and to provide theoretical basis for the treatment and basic research of related diseases correspondingly.
    Breeding & Cultivation
    Breeding and identification of a wide-compatibility line HDH73 in rice
    XIA Ming-yuan, LI Jin-bo, QI Hua-xiong
    2020, 59(24):  21-23.  doi:10.14088/j.cnki.issn0439-8114.2020.24.004
    Asbtract ( 452 )   PDF (1505KB) ( 188 )  
    References | Related Articles | Metrics
    The exploration of heterosis in indica-japonica hybrids is an important approach to increasing the yield potential of rice (Oryza sativa L.). Hybrid sterility was a major obstacle for utilization of such heterosis. The wide-compatibility genes provided valuable genetic resources to effectively solve the problem of hybrid sterility for rice breeding. An intermediate line HDH73, which had good agronomic characteristics and wide compatibility gene, was bred by anther culture with indica-japonica hybrid Yongyou 4949 as donor. The high-throughput SNP (single nucleotide polymorphism) genotyping showed that the indica/japonica classification of HDH73 was partial japonica, and the frequency of japonica loci of HDH73 accounted for about 2/3 by SNP analysis with polymorphism marker between indica and japonica. The hybrids of Guang 3S/HDH73 and Changlijing S/HDH73 were planted and investigated agronomic characteristics. The growth stages and plant heights of these hybrids were similar to those of the medium-indica hybrids which were widely planted, and the seed setting rate was fertile. The rice line HDH73 could be wide-compatibility resource in breeding program for utilization of inter-subspecific heterosis.
    The relationship among phenotype, heterosis and combining ability of hybrid rice
    CHEN Jun-xiao, YOU Ai-qing, LIU Kai
    2020, 59(24):  24-28.  doi:10.14088/j.cnki.issn0439-8114.2020.24.005
    Asbtract ( 655 )   PDF (2647KB) ( 295 )  
    References | Related Articles | Metrics
    We constructed a NCII population and performed a high-throughput phenotyping facility (HRPF) and combined with artificial investigation to measure all traits, to investigate the relationship between phenotype, general combining ability, special combining ability and heterosis of yield-related traits. The results showed that there were high correlations among phenotype, SCA, GCA and heterotic advantage for all traits. The phenotype was always positively correlated with GCA and SCA, but GCA and SCA contributed variously to different agronomic traits;heterotic advantage always positively correlated with GCA and SCA, and SCA contribute more to the heterosis. GCA always negatively or not correlated with SCA, the correlations with heterosis and parent genetic distance (PGD) and observed heterozygosity (OH) were not particularly evident in our data.
    Effects of different sowing date and planting density on yield and agronomic characters of E-Jing 403
    LI Pei-de, YANG Guo-cai, LI Zhen-lian, LIU Kai, CHEN Zhi-jun, ZHOU Lei, XU Hua-shan, ZHA Wen-jun, LI San-he, YOU Ai-qing
    2020, 59(24):  29-31.  doi:10.14088/j.cnki.issn0439-8114.2020.24.006
    Asbtract ( 417 )   PDF (1510KB) ( 168 )  
    References | Related Articles | Metrics
    To investigate the effects of different sowing date and planting density on yield and agronomic characters of direct-seeding rice after wheat harvest, japonica variety E-Jing 403 was grown in field experiment with 3 sowing dates S1(05-25), S2(06-04), S3(06-14)and 4 densities D1(45 kg/hm2), D2(60 kg/hm2), D3(75 kg/hm2) and D4(90 kg/hm2). The results showed that the sowing date has a great influence on the whole growing period of E-Jing 403. With the postponing of sowing date, the whole growing period is shortened. Direct seeding density had extremely significant effects on basic seedlings, highest seedlings and effective ears,while sowing date had extremely significant effects on plant height, percentage of available tiller and yield. In order to improve the yield of direct seeding rice E-jing 403, the seeding rate should be reduced in early sowing and increased in late sowing.
    Evaluation of high and stable yield ability of wheat varieties in middle and lower reaches of Yangtze river by high-stable yield coefficient method
    ZHANG Yu-qing, ZHU Zhan-wang, LIU Yi-ke, CHEN Ling, ZOU Juan, TONG Han-wen, HE Wei-jie, ZHU Guang, LI Xiang-cheng, WANG Wen-xue, GAO Chun-bao
    2020, 59(24):  32-34.  doi:10.14088/j.cnki.issn0439-8114.2020.24.007
    Asbtract ( 454 )   PDF (1506KB) ( 233 )  
    References | Related Articles | Metrics
    The high and stable yield ability of 26 wheat varieties (lines) in the regional trial of the middle and lower reaches of the Yangtze river in 2016-2017 were analyzed by using the high-stable yield coefficient (HSC) method. The results showed that the evaluation results acquired by HSC method were basically consistent with that of conventional statistical analysis. But HSC can measure the consistency of high and stable yield of varieties more simply and intuitively through HSC value. After analysis, Huihong 225 was evaluated as a typical high and stable yield variety as its yield, HSC value and coefficient of variation ranked the first, the first and the second respectively. So it should have a wide popular prospect in the future.
    Analysis of agronomic characters and quality performances on wheat germplasm of different types planted in humpy ground in northern Hubei province
    LIU Ying, JIN Xiao-jie, LING Dong, ZUO Zhen-xi, TAN Jing, LONG Xiao-ling , WEN Liang, WANG Li-feng
    2020, 59(24):  35-40.  doi:10.14088/j.cnki.issn0439-8114.2020.24.008
    Asbtract ( 485 )   PDF (1542KB) ( 286 )  
    References | Related Articles | Metrics
    The performance and variation of wheat (Triticum aestivum L.) quality and agronomic characters on wheat germplasm of different types planted in humpy ground in Xiangyang of northern Hubei province were studied. We have analyzed the agronomic characters and quality indices. The variation analysis and correlation analysis were made on five agronomic character indices including grain number per spike, 1 000-grain weight, plant height and 11 wheat quality indices such as protein content, wet gluten content and sedimentation value. The results of variation analysis showed that the grain number per spike had the highest variation, while the least variation was found on 1 000-grain weight and spikelet number. As for quality indices, the bulk density was with the smallest variation while stability time had the highest variation. Correlation analysis showed that significant positive correlations among the agronomic characters and quality indices. It should be considered in wheat breeding and germplasm resources innovation to improve the selection efficiency of high quality wheat and the accuracy of comprehensive evaluation.
    Analysis and evaluation on tuber quality of different potato varieties
    ZHU Jie, SU Xiu-li, WU Guo-wen, FANG Zhi-guo, WEN Hai-xia, ZHANG Jie, FENG Peng, WANG Si-qing
    2020, 59(24):  41-44.  doi:10.14088/j.cnki.issn0439-8114.2020.24.009
    Asbtract ( 505 )   PDF (1517KB) ( 286 )  
    References | Related Articles | Metrics
    In order to select new potato varieties with good quality, a set of comprehensive analysis and evaluation methods of potato quality was put forward, and the nutritional quality, processing quality and commodity quality of 6 potato varieties were evaluated. The results showed that Hongmei had the best nutritional quality, Huashu No. 3 had the best processing quality and commodity quality, and Huashu No. 3 was the best in comprehensive quality analysis.
    Resource & Environment
    Study on fertilization effect and appropriate fertilizer application of green manure rapeseed
    ZHOU Yu-ting, TIAN Gui-sheng, YOU Jiang-feng, WU Hai-ya, CHEN Yan, YOU Shao-yang
    2020, 59(24):  45-47.  doi:10.14088/j.cnki.issn0439-8114.2020.24.010
    Asbtract ( 453 )   PDF (1512KB) ( 197 )  
    References | Related Articles | Metrics
    In order to find out the application effect and suitable application amount of fertilizer on green manure, six different fertilization treatments were set up to study the effects of different fertilization dosages on the phenological period, winter solstice and biomass of green manure rapeseed. The results showed that fertilization not only prolonged the growth period of green manure and improved winter solstice, but also had a certain effect on improving the biomass of green manure. Compared with the treatment without fertilization (T1), the growth period of green-fertilized rapeseed treated with fertilization (T2 to T6) was delayed by 2~5 days, and the plant heightincreased by 6.3~9.7 cm, rhizome thicknessincreased by 4.7~5.0 mm, leaf number per plant increased by 1.7~2.8 tablets,and leaf area of winter saplings increased by 21.1~41.3 cm2; Compared with T1, the biomass fresh weight of vegetated vegetable moss increased by 14 775~31 410, 16 290~33 585 kg/hm2, and dry weight of vegetated vegetable moss increased by 9 300~21 990 and 10 140~24 345 kg/hm2 respectively at the mature stage and in the fertilized vegetable moss stage. The optimal fertilizer rate of green rape in Wuxue city was N 75 kg/hm2, P2O5 30 kg/hm2 and K2O 45 kg/hm2.
    Complete experimental analysis of fertilizer effect of waterlogged paddy soil rice “3414” in Zhongxiang city
    FENG Jing, LI Jing-rong, GUAN Shao-hua, LI Hong-jun
    2020, 59(24):  48-50.  doi:10.14088/j.cnki.issn0439-8114.2020.24.011
    Asbtract ( 454 )   PDF (1512KB) ( 152 )  
    References | Related Articles | Metrics
    “3414” fertilizer effects test design scheme was adopted to carry out field experiments to explore the optimal amount and ratio of nitrogen, phosphorus and potassium fertilization in waterlogged paddy soil rice. The current recommended amount of nitrogen fertilizer (N 180.0 kg/hm2), amount of phosphorus fertilizer (P2O5 67.5 kg/hm2) and amount of potassium fertilizer (K2O 90.0 kg/hm2) on rice yield increase was studied. The results showed that fertilization increased the yield significantly, with an average yield increase of 235.1~3 066.6 kg/hm2, with growth of 3.5%~45.3%. Fertilization increased the gross income by 587.8~7 666.5 yuan/hm2, increased the investment by 886.5~2 361.1 yuan/hm2, the yield to investment ratio was 0.7~3.9, and the net income was -298.5~5 697.0 yuan/hm2. The agronomic utilization rate of nitrogen fertilizer was 8.7~19.5 kg/kg, 13.5 kg/kg for P2(P2O5 67.5 kg/hm2) and 4.8 kg for P3(101.3 kg/hm2), and 6.5 kg/kg for K2(90.0 kg/hm2).
    Effects of nitrogen application on yield increase, nutrient absorption and nitrogen use efficiency of wheat in Hubei province
    ZOU Juan, LI Xiang-cheng, ZHANG Zi-hao, TANG Hao-jun, WANG Peng, YAN Shuang-yi, ZHANG Yu-qing, GAO Chun-bao
    2020, 59(24):  51-55.  doi:10.14088/j.cnki.issn0439-8114.2020.24.012
    Asbtract ( 506 )   PDF (1565KB) ( 160 )  
    References | Related Articles | Metrics
    32 field experiments were conducted in wheat areas in the hills of central Hubei and highland of north Hubei(CNH) and Jianghan plain(JP) from 2015 to 2018. Three treatments were set, namely CK(no fertilization), N0PK(no nitrogen application) and NPK(combined application of nitrogen, phosphorus and potassium). The grain yield, stem and leaf biomass of wheat(Triticum aestivum L.) were measured at the maturity stage, and the contents of nitrogen, phosphorus and potassium in each part were analyzed. The results were indicated that application of N fertilizer increased wheat yield significantly, the average grain yield increment resulting from N application was 1 838 kg/hm2, and increase rate was 58.2%. N contribution rate to wheat was 36.8%. The effect of yield increasing by N application in CNH was more significant than that in JP. Compared to N0PK treatment, N, P2O5 and K2O uptakes of NPK treatment at harvest were increased by 72.97%, 56.19% and 83.13%. N recovery efficiencyof wheat in Hubei province was 34.0%, and agronomic N use efficiency, N physiological efficiency of wheat in Hubei province were 10.2 kg/kg and 33.0 kg/kg, respectively. N use efficiencies of wheat in CNH were higher than that in JP. Nutrient absorptions per 100 kg grain in Hubei province averaged 2.87 kg N, 1.02 kg P2O5 and 2.51 kg K2O, respectively. The combination of N with P and K fertilizers could increase the shoot biomass and nutrient uptake significantly, the grain yield of wheat in different region of Hubei province also improved simultaneously. At the same level of wheat yield, N and P2O5 absorptions were high in JP, and K2O was high in CNH.
    Rice fertilizer reduction effect assessment study based on fuzzy hierarchical analysis: Take Hubei province as an example
    YANG Li, MA Chao-hong, HU Shi-you, DUAN Jian-she, XU Wei-ming, TU Tao
    2020, 59(24):  56-62.  doi:10.14088/j.cnki.issn0439-8114.2020.24.013
    Asbtract ( 456 )   PDF (1587KB) ( 230 )  
    References | Related Articles | Metrics
    By constructing a fuzzy comprehensive evaluation model, using the technical, economic, social and environmental indicators, organic fertilizer instead of fertilizer volume, fertilizer application reduction value, the effect of rice fertilizer reduction in rice rotation area of Hubei province was evaluated by 14 three-level indicators, such as the degree of government policy support response and the improvement of soil environment in farmland. The overall effect evaluation was better, among which the technical, economic and social evaluation was better, and the environmental effect evaluation was very good.
    Effects of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning on yield and nutrient accumulation of kidney bean
    XU Da-bing, XIE Yuan-yuan, XIA Xian-ge, ZHOU Jian-xiong, YUAN Jia-fu, ZHAO Shu-jun
    2020, 59(24):  63-66.  doi:10.14088/j.cnki.issn0439-8114.2020.24.014
    Asbtract ( 420 )   PDF (1784KB) ( 127 )  
    References | Related Articles | Metrics
    Field experiment was carried out to study the effects of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning and fertilizer reduction on yield on yield and nutrient accumulation of kidney bean. The results showed that compared with conventional fertilization, the yield of kidney bean increased by 13.41% and 29.43% with the half and all Brassica campestris L. ssp. chinensis var. utili Tsen et Lee waste returning and conventional fertilization, respectively. On the basis of returning all the waste of Brassica campestris L. ssp. chinensis var. utili Tsen et Lee, the yield decreased by 25.41% of the treatments with 30% reduction of chemical fertilizer and all waste returning. The biomass increased by 9.38% of the 30% reduction of chemical fertilizer and all waste returning treatments comparing to conventional fertilization. When compared with conventional fertilization, the nitrogen, phosphorus and potassium accumulation increased by 18.72%~32.55%, 6.35%~38.22%, 5.73%~41.87% of waste returning treatments, and the nitrogen and potassium mainly existed in stems, leaves and fruits.
    Analysis of the timing characteristics and influencing factors of agricultural carbon emissions in Hubei province
    ZHANG Jin-xin, HU Wan-ling, WANG Hong-ling
    2020, 59(24):  67-74.  doi:10.14088/j.cnki.issn0439-8114.2020.24.015
    Asbtract ( 458 )   PDF (1709KB) ( 147 )  
    References | Related Articles | Metrics
    Agricultural carbon emissions in Hubei province from 1993 to 2017 using 6 types of carbon sources was calculated, including fertilizers, pesticides, agricultural films, agricultural diesel, agricultural planting area, and agricultural irrigation area. By analysing of time-series characteristics and LMDI addition and decomposition, the influencing factors of agricultural carbon emissions were studied in Hubei province. The results showed that Hubei's agricultural carbon emissions generally increased first and then declined between 1993 and 2017, reaching its peak in 2012, but with a significant upward trend, and agricultural carbon emissions increased with a significant downward trend. The carbon emissions and increments per unit of GDP had a significant downward trend, the carbon emissions per unit of arable land had a significant upward trend, and the carbon emissions per unit of arable land had a significant downward trend; From the decomposition results of the influencing factors, agricultural production efficiency, industrial structure, rural population and agricultural industrial structure were the main influencing factors of agricultural carbon emission reduction in Hubei province, and their contributions decrease in turn, while the level of economic development and urbanization were the main influencing factors leading to the increase of agricultural carbon emission in Hubei province.
    Content and spatial variability of nitrogen and phosphorus in dich sediment in a typical small area of Jianghan plain
    NIE Yu-lian, XIONG Gui-yun, LIU Dong-bi, HUANG Min, FAN Xian-peng, WU Mao-qian, XIA Ying, LIU Yi
    2020, 59(24):  75-81.  doi:10.14088/j.cnki.issn0439-8114.2020.24.016
    Asbtract ( 367 )   PDF (2274KB) ( 218 )  
    References | Related Articles | Metrics
    A typical small area naturally enclosed by drainage and irrigation canals was selected to monitor and analyze the content and spatial variability of nitrogen and phosphorus in 0~10 cm sediment. The results showed that in the 36 sediment samples, the total nitrogen content was 0.7~2.9 g/kg, the average value was 1.79 g/kg. The total soluble nitrogen content was 17~166 mg/kg, the proportion of inorganic nitrogen in total soluble nitrogen was 88.1%~94.5%, the main part of inorganic nitrogen was ammonium nitrogen, the proportion of which was 84.7%~90.4%. The total phosphorus content of the ditch sediment was 0.4~1.3 g/kg, which averaged 0.60 g/kg, the Olsen-P was 10.2~131.7 mg/kg, accounting for 2.6%~10.1% of the total phosphorus, and the water-soluble phosphorus content was 0.5%~3.7% of Olsen-P. The total nitrogen, total soluble nitrogen and ammonium nitrogen, as well as total phosphorus, Olsen-P and water-soluble phosphorus in the ditch sediment showed a very significant positive correlation at the level of α=0.01. The average value of various form nitrogen in the ditch sediment was the highest in the laternal canal near the fish pond, while the highest phosphorus content was observed in the branch canal near the residential area.
    Analysis of agricultural climate resources change during mid-season rice growth period in Suizhou city
    YANG Tao, FENG Wen-li, BAO Feng-xia, ZHANG Zhi-sheng, CHENG Jian-ping
    2020, 59(24):  82-87.  doi:10.14088/j.cnki.issn0439-8114.2020.24.017
    Asbtract ( 409 )   PDF (2218KB) ( 276 )  
    References | Related Articles | Metrics
    Based on the daily ground surface meteorological observation data of two national meteorological stations in Suizhou city from 1959 to 2018, the variation characteristics of agricultural climate resources such as sunshine, temperature and precipitation in this region under the background of climate change were calculated and analyzed. The results showed that the climatic tendency rate of accumulated temperature ≥10 ℃ in Suizhou was 30.6 (℃·d)/10 years, with a significant increasing trend. The trend rate of accumulated temperature ≥10 ℃ in Guangshui city was 82.5 (℃·d)/10 years. There was no significant increase trend of accumulated temperature ≥20 ℃ in Suizhou city; The cumulative temperature ≥20 ℃ had a significant increasing trend, and the climatic tendency rate ≥20 ℃ was 89.2 (℃·d)/10 years. The climate tendency rate of sunshine hours in Suizhou city and Guangshui city were -35.4 h/10 years and -25.2 h/10 years, respectively, and both showed a significant decreasing trend. The trend of solar radiation reduction in Suizhou city was significant, and the trend rate of solar radiation climate was -42.4 MJ/(m2·10 years), while the trend of solar radiation reduction in Guangshui city was not significant. The annual precipitation fluctuated greatly in the growing season and the variation trend was not significant. The climate tendency rate of reference evapotranspiration in Suizhou city was -24.7 mm/10 years, and the variation trend of reference evapotranspiration in Guangshui city was not significant.
    Effects of planting years and cultivation methods on heavy metal accumulation in vegetable soil
    WANG Su-ping, DU Lei, GUO Cui-ying, HUANG Xiang, ZHANG Gui-you, HONG Juan, LIAN Zhi-cheng, JIANG Li, ZHANG Li-hong, YE Li-xia, CHEN Gang
    2020, 59(24):  88-91.  doi:10.14088/j.cnki.issn0439-8114.2020.24.018
    Asbtract ( 462 )   PDF (1496KB) ( 134 )  
    References | Related Articles | Metrics
    In order to study the effects of different planting years and cultivation methods on the accumulation of heavy metals and their bioavailability, the contents and bioavailability coefficients of 8 heavy metals (As, Cd, Cr, Cu, Ni, Pb, Zn, Hg) in vegetable soil with planting years less than 5, 5~10, 10~15 years and 15~20 years were studied. At the same time the content of heavy metals and bioavailability coefficient of soil under open and protected cultivation were analyzed. The results showed that the content of As, Cd, Ni and Pb increased with the increase of planting years, the effective state content and bioavailability of Cd, Cr, Cu, Pb and Zn were the highest and biggest in 10~15 years, the content of As, Cd, Hg and Ni was higher culture in open field than in the greenhouse soil, the content of Cr, Cu, Pb and Zn was higher culture in greenhouse soil than in open field. The bioavailability coefficient of Ni elements culture in the open field was higher than that in the greenhouse soil, and that of As, Cr, Cd, Cu, Hg, Pb, Zn elements culture in the greenhouse soil was higher than that in the open field. The average contents of As, Cr, Cu, Pb and Zn in the soil planted with leafy vegetables were higher than those with fruits, fruits and rhizomes. The average content of Hg was the highest in the soil planted with melons and fruits, and the average contents of Cd and Ni were the highest in the soil planted with rhizome vegetables.
    Analysis and ecological risk assessment on heavy metal contents of farmland soils in Wuhan East Lake High-Tech Development Zone
    ZHANG Gui-you, DU Lei, WANG Su-ping, HUANG Xiang, JIANG Li, HONG Juan, LIAN Zhi-cheng, ZHANG Li-hong, YE Li-xia,CHEN Gang
    2020, 59(24):  92-95.  doi:10.14088/j.cnki.issn0439-8114.2020.24.019
    Asbtract ( 463 )   PDF (1519KB) ( 122 )  
    References | Related Articles | Metrics
    A total of 58 soil samples were collected from farmland soils in Wuhan East Lake High-Tech Development Zone. The contents of 8 heavy metals (Pb, Cd, Cr, Hg, As, Ni, Cu, Zn) were analyzed. The single factor pollution index and Nemerow synthetical pollution index were used to evaluate the heavy metals in farmland soils in this area based on the soil environmental quality risk management and control standard. The results showed that the farmland soil in Wuhan East Lake High-Tech Development Zone was in a clean level, and there was slight Cd pollution in a small area (1.72%). There was no Pb, Ni, Cr, Cu, Hg, Pb pollution were found.
    Plant Protection
    A field efficacy of jinggangmycin and thifluzamide on corn sheath blight
    YUAN Wei-jun, ZHOU Zhong-xiong, ZHOU Zhong-quan, CHENG Ting-ting, LIU Mei-ling, XIANG Li-bo
    2020, 59(24):  96-98.  doi:10.14088/j.cnki.issn0439-8114.2020.24.020
    Asbtract ( 971 )   PDF (1512KB) ( 286 )  
    References | Related Articles | Metrics
    To evaluate the control effects and the effects on yield of jinggangmycin and thiofuramide on maize sheath blight, the field trials were carried out by spraying in early stage of maize sheath blight. The results showed that 24% of jinggangmycin AS 600, 1 200 mL/hm2 and 240 g/L thifluzamide SC 150, 300, and 600 mL/hm2 all had better control efficacy on corn sheath blight. The control effects was 77.90% to 88.53%, and 1 000-grain weight was significantly higher than that of the control.
    Identification of root rot diseases from Hippeastrum rutilum
    MENG Qing-zhong, LI Xiao-jian, YI Xian-da, LI Guo-rong, ZHANG Tao, LYU Bo
    2020, 59(24):  99-100.  doi:10.14088/j.cnki.issn0439-8114.2020.24.021
    Asbtract ( 468 )   PDF (2034KB) ( 154 )  
    References | Related Articles | Metrics
    The pathogen from the root tissue of Hippeastrum rutilum was isolated by tissue isolation method, and the pathogen was identified by morphological observation and ITS sequence analysis. The results showed that the colony of the pathogen was round, the hyphae were white villous at first, and gradually changed to light yellow with time, and the conidia of the pathogen strain had two forms, the large conidia were straight or slightly curved into sickle shape, there were more transverse septum, and the small conidia were oval or oval with 1~2 transverse septum. The pathogen was identified as Fusarium solani by morphology, pathogenicity determination and ITS sequence analysis.
    The identification and screening of fungicides against Fusarium culmorum from stem of walnut
    WANG Peng-cheng, FANG Ai-ping, QU Ding-bin, PENG Xin, JIANG Li, JIN Tai-xing, SUN Chang-cheng, YANG Zheng-rong, JIA Qie
    2020, 59(24):  101-105.  doi:10.14088/j.cnki.issn0439-8114.2020.24.022
    Asbtract ( 632 )   PDF (1829KB) ( 158 )  
    References | Related Articles | Metrics
    In recent years, the black rot on young walnut stems has seriously affected the sound development of the industry in this area. In order to clarify the pathogen and improve the effect of managing this disease, identification and screening of the fungicide against the pathogen from walnut young stem were carried out in laboratory. The results showed that the black rot on walnut was mainly caused by Fusarium culmorum and the pathogen was sensitive to different fungicides. Among them, the toxicity of 40% flusilazole emulsifiable concentrate was the strongest one with an EC50 of 0.383 7 μg/mL, followed by 43% tebuconazole suspension with an EC50 of 9.192 1 μg/mL, and 70% thiophanate methyl wettable powder with an EC50 of 2 010.961 μg/mL. In the practice of pathogen control, 40% flusilazole emulsifiable concentrate and 43% tebuconazole suspension should be considered as the main control agents Fusarium culmorum from walnut stem.
    Study on the screening method of microbial herbicide activity
    ZHANG Zhi-gang, WANG Kai-mei, WU Zhao-yuan, WAN Zhong-yi, FANG Wei
    2020, 59(24):  106-109.  doi:10.14088/j.cnki.issn0439-8114.2020.24.023
    Asbtract ( 340 )   PDF (2060KB) ( 288 )  
    References | Related Articles | Metrics
    Using [Cynodon dactylon (L.) Pers.] and (Amaranthus retroflexus L.) as materials three different screening methods were used to screen the activity of microbial herbicides in laboratory. The results showed that the microplate method was suitable for the screening of quick acting compounds or active ingredients. It had the advantages of large screening flux, strong operability and less sample dosage. However, it could be used for the preliminary screening of compound activity due to its short culture time and poor anti pollution ability, but it was not suitable for the screening of microbial fermentation broth or living bacteria samples; The metabolism of the leaves in vitro is slower than that of the leaves in vivo, and the growth point almost stops growing, which can not fully reflect the activity of the samples to the leaves in vivo; The mini pot method is suitable for screening samples with complex components. The results are close to the advantages of field medication, strong pollution resistance and long observation time, but the dosage is relatively large, the operation is complex, and the screening flux is limited.
    Secondary metabolites from fungus NBERC_51984 with herbicidal activity
    WU Zhao-yuan, ZHANG Zhi-gang, ZHANG Ya-ni, LIU Fang, FANG Wei
    2020, 59(24):  110-112.  doi:10.14088/j.cnki.issn0439-8114.2020.24.024
    Asbtract ( 417 )   PDF (1558KB) ( 205 )  
    References | Related Articles | Metrics
    In the search for pesticides of natural products from microbes, fungus NBERC_51984 with herbicidal activity was obtained. In order to clarify the active substance basis of fungus NBERC_51984, its secondary metabolites were studied. By activity tracking and large-scale fermentation, 2 compounds were isolated and identified as Pseurotin A (1) and Bisdethiobis(methylthio)gliotoxin (2) by LC-MS and NMR. Pseurotin A was found to show herbicidal activity to Amaranthus retroflexus.
    Preliminary study on degradation of acetochlor residue in soil by different types of organic fertilizer
    CHANG Xiang-qian, HAN Mei-mei, LIU Dong-bi, ZHANG Shu
    2020, 59(24):  113-116.  doi:10.14088/j.cnki.issn0439-8114.2020.24.025
    Asbtract ( 426 )   PDF (1702KB) ( 163 )  
    References | Related Articles | Metrics
    After the paddy soil in the barrel was treated with different types of organic fertilizer, the solution of acetochlor with the concentration of 1.2 g/L was sprayed, and the soil samples were taken to detect the residue of acetochlor at different days (0.5, 1, 3, 5, 7, 14, 21, 30, 45 d). The half-lifes of acetochlor after different treatments were calculated, and the feasibility of degradation of acetochlor by fertilizer treatment was analyzed. The results showed that after the application of organic water-soluble fertilizer or microbial organic fertilizer, the half-life of acetochlor in soil was significantly shorter than that of the control. After PSBGH, the half-life of acetochlor was the shortest, which was 21.77% of that of the control. After the application of AWJL, the half-life of acetochlor was the longest in all treatments, which was 61.51% of the control. It was considered that the application of organic fertilizer could be used as an effective method to degrade acetochlor residues in paddy fields.
    Identification and biological characteristics of the pathogen causing anthracnose on goldthread in Hubei province
    WANG Zuo-qian, PAN Zhu-qing, ZHANG Shu, YOU Jing-mao, WANG You-ping, YANG Xiao-lin
    2020, 59(24):  117-121.  doi:10.14088/j.cnki.issn0439-8114.2020.24.026
    Asbtract ( 382 )   PDF (2035KB) ( 245 )  
    References | Related Articles | Metrics
    To identify anthracnose on goldthread in Hubei province, study its biological characteristics, and screen effective fungicides, there were twelve isolates isolated by tissue isolation method from infected leaves collected from Banqiao, Enshi, Hubei province. Among them, EsH2 and EsH6 succeed to infect leaves of goldthread in vitro and produce typical lesions. While morphology and ITS phylogenetic analysis indicated the two isolates belonging to Colletotrichum boninense species complex. Evaluation of culture under variants illumination conditions showing that they grew significantly slower under absence of light. And they grew more rapid on mung bean medium and PDA than obtained from others, respectively. Results of fungicide toxicity assay exhibited thiophanate-methyl, pyraclostrobin and difenoconazole were more effective fungicides than others with relatively low EC50 and high correlation coefficient.
    Occurrence regularity and integrated control technology of citrus canker
    WANG Zhi-jing, WU Li-ming, SONG Fang, HE Li-gang, WANG Ce, JIANG Ying-chun
    2020, 59(24):  122-123.  doi:10.14088/j.cnki.issn0439-8114.2020.24.027
    Asbtract ( 445 )   PDF (2073KB) ( 161 )  
    References | Related Articles | Metrics
    Citrus canker is a kind of quarantine bacterial disease, which seriously damages its leaves, branches and fruits, and then leads to the decrease of citrus yield. At the same time, it affects the commodity and sales of citrus. It has the characteristics of rapid transmission and difficult to cure. the symptom recognition and occurrence regularity of citrus canker weresummarized, the comprehensive control technology waselaborated, in order to effectively carry out prevention and control, reduce the cost of prevention and control, make the citrus production can be smoothly developed.
    Control effects of five kinds of fungicides against the wheat rhizome rot disease
    WANG Hua, ZHANG Su-mei, CHANG Wei, ZHANG Xue-jiang
    2020, 59(24):  124-127.  doi:10.14088/j.cnki.issn0439-8114.2020.24.028
    Asbtract ( 1805 )   PDF (1500KB) ( 460 )  
    References | Related Articles | Metrics
    The study aims to find out high effective fungicides which had better control on wheat rhizome rot disease in filed in Honghu city, Hubei Province. Two methods were used: seed dressing and root spraying. The results showed that the control effects of five kinds of fungicides (50% carbendazim, 43% tebuconazole, 19% propiconazole, 21% jinggangmycin,50% formosa) in the seed dressing test were 25.3%~56.9%. Among them, 43% tebuconazole (1500 ml/hm2) showed the highest control effect (56.9%). At the same, the control effect of 19% propiconazole (900 g/hm2) and 21% jinggangmycin (750 mL/hm2) was also higher (53.1% and 50.0%, respectively). The control effects of five kinds of fungicides (60% carbendazim, horizon (430 g/L), 19% propiconazole, 24% jinggangmycin,weifu (400 g/L)) in the root spraying test were 11.8%~54.4%. Among them, the horizon (430 g/L) (180 g/hm2) showed the highest control effect (54.4%). The control effects of 19% propiconazole (900 mL/hm2) and horizon (430 g/L) (135 g/hm2) were also higher (49.3% and 43.2%, respectively). To control the wheat rhizome rot in Honghu city, the horizon (430 g/L) (180 g/hm2) and 19% propiconazole (900 mL/hm2) for seed dressing should be selected; 43% tebuconazole (180 g/hm2) and 19% propiconazole (900 mL/hm2) for the root spraying should be selected.
    Horticulture & Local Products
    Growth regularity of precious wood species Phoebe zhennan in Chongqing city
    XUE Pei-pei, CHEN Ben-wen, ZHU Yuan-chun, QIAN Tao
    2020, 59(24):  128-132.  doi:10.14088/j.cnki.issn0439-8114.2020.24.029
    Asbtract ( 734 )   PDF (1576KB) ( 406 )  
    References | Related Articles | Metrics
    Based on the material of stem analysis, Phoebe zhennan natural secondary forest of state forest farm in Yongchuan district of Chongqing city was analyzed. The results showed that,the mean DBH of 60-year Phoebe zhennan was 38.81 cm, mean annual DBH increment was between 0.63 cm and 0.81 cm. The mean height of 60-year Phoebe zhennan was 35.30 m, mean annual height increment was 0.68 m. The maximum of current annual DBH increment and height increment were 1.00 cm and 1.00 m, and they were occurred from 15 years to 20 years, the minimum of current annual DBH increment and height increment were occured in 55~60 years, they were 0.31 cm and 0.14 m, respectively. Until the 60th year, the growth of volume is in a state of continuous growth. The volume of 60-year Phoebe zhennan was reached 1.713 1 m3, mean annual volume increment was 0.017 4 m3. The maximum of current annual volume increment occurred at 45-year-old and it was 0.047 5 m3. The growth dynamic models of height,DBH and volume of Phoebe zhennan were established with the cubic polynomial, the goodness of fit all exceed 99%. Three regression equations were y=-5.275+1.203x-0.007x2-0.000 006x3,y=-5.109+1.087x-0.004x2-0.000 06x3,y=0.182-0.035x+0.002x2-0.000 002x3, where y is DBH, height and volume, x is age. Comparing with fast growing tree species Cunninghamia lanceolata, the height and DBH increment of 38-year Cunninghamia lanceolata were 18.34 m and 25.52 cm, the tree height and DBH growth rate of Phoebe zhennan were higher than that of Cunninghamia lanceolata, in addition to the high velue of Phoebe zhennan wood, it can be widely cultivated and applied as the precious tree species in China.
    Study on the dihydromyricetin in Ampelopsis grossedentata
    ZHENG Lin, GAO Shi-wei, LIU Pan-pan, ZHENG Peng-cheng, GONG Zi-ming
    2020, 59(24):  133-134.  doi:10.14088/j.cnki.issn0439-8114.2020.24.030
    Asbtract ( 509 )   PDF (1505KB) ( 169 )  
    References | Related Articles | Metrics
    The content of dihydromyricetin(DMY)in Ampelopsis grossedentata from different areas was comparatively analyzed,the results showed that the content of DMY was ranked Lijiang city in Yunnan province>Jinxiu Yao Autonomous county of Guangxi Zhuang Autonomous region> Wuping countyin Fujian>Zhangjiajie city in Hunan province>Laifeng county of Enshi Tujia and Miao Autonomous Prefecture from high to low. The content of DMY in Ampelopsis grossedentata from Lijiang city in Yunnan province was significantly highest, and the content of DMY from Laifeng county of Enshi Tujia and Miao Autonomous Prefecture was significantly lowest,there was no significant difference among other areas. On the basis, DMY was extracted from Ampelopsis grossedentata by ultrasonic, microwave, wall breaking methods with ethanol,the results showed that microwave-assisted ethanol extraction could achieve green, energy-saving and high-efficiency extraction state with less amount of ethanol, shorter extraction time and lower solid-liquid ratio.
    Investigation and preliminary analysis on nutritional factors of soil, leaf and fruit in citrus orchards in Hubei province
    HE Li-gang, WU Li-ming, JIANG Ying-chun, ZHANG Lian-lian, JIANG Yan, YAO Jing-lei, WANG Zhi-jing, SONG Fang
    2020, 59(24):  135-140.  doi:10.14088/j.cnki.issn0439-8114.2020.24.031
    Asbtract ( 452 )   PDF (1524KB) ( 203 )  
    References | Related Articles | Metrics
    The nutrition factors of soil, leaf and fruit, and quality of fruits in citrus orchards from Yidu, Dangyang, and Zigui county in Hubei province were investigated and analyzed, referred to the standard of diagnostic on citrus nutrition, the results demonstrated that boron (B) was deficient in soil of Yidu and Dangyang, where manganese (Mn) was excessive while zinc (Zn) was deficient, calcium (Ca) was excessive in soil of Zigui; the soil from three different areas contained insufficient nitrogen (N) and Zn, but too much Mn; due to the main cultivars in above areas, the nutritional levels were different from each other. Based on the above conclusion, the soil environment and plant nutritional levels were determined and theoretically contributed to the nutritional diagnostic, fertilizing efficiency and ecological improvement for the development of the citrus industry in Hubei province.
    Animal Husbandry & Veterinary Medicine
    Polymorphism of porcine OLR1 gene and its association with liter size trait of Xidu black pigs
    QIAO Mu, LI Kang-chi, WU Jun-jing, WU Hua-yu, ZHOU Jia-wei, MEI Shu-qi, PENG Xian-wen
    2020, 59(24):  141-143.  doi:10.14088/j.cnki.issn0439-8114.2020.24.032
    Asbtract ( 434 )   PDF (1622KB) ( 195 )  
    References | Related Articles | Metrics
    In order to explore the effect of OLR1 gene genetic polymorphism on litter size of Xidu black pigs, the direct sequencing method was used to detect the genetic polymorphism of OLR1 gene in the Xidu black pigs, and the association analysis with litter size was carried out. The results showed that there was a T/C mutation at 7 bp of intron 4, and there were three genotypes TT, TC and CC; The total number born and number born alive of individuals with TT genotype were extremely significantly higher than those with TC and CC genotype (P<0.01), indicating that the mutation had a significant impact on litter size traits of Xidu black pig.
    Identification of HDLBP SNPs and its association with egg performance traits
    HUANG Tao, CHENG Lu, MO Chang-huan, WANG Xiao-zhong, SHEN Jie, PI Jin-song, GONG Yan-zhang
    2020, 59(24):  144-147.  doi:10.14088/j.cnki.issn0439-8114.2020.24.033
    Asbtract ( 379 )   PDF (1696KB) ( 174 )  
    References | Related Articles | Metrics
    6 SNPs in the full length of HDLBP transcript were identified as a synonymous mutation in adult hens. Association between two SNPs and egg performance traits were analyzed in Xinhua E-strain chickens, which showed that they were highly significantly associated with AFE and EW (P<0.05), suggesting that it was a candidate gene related with egg production. The HDLBP mRNA in different sized follicles showed that the expression was progressively increased in follicles from undifferentiated follicles to SYFs, and maintain stable in pre-ovulatory follicle. Besides, FSH, EGF, VEGF with dosage dependent could regulate the expression of HDLBP in granulosa cells of phases, while Follistatin showed no effect.
    Storage & Processing
    Study on the extraction technology of essential oil from Damascus rose
    YANG Qing, LIU Xing-le, LI Li-yun, WANG Dun, WANG Xue, ZHANG Shu-juan
    2020, 59(24):  148-150.  doi:10.14088/j.cnki.issn0439-8114.2020.24.034
    Asbtract ( 453 )   PDF (1500KB) ( 161 )  
    References | Related Articles | Metrics
    The extraction technology of essential oil from Damascus rose was studied by steam distillation. The effects of dosage, extraction time, ratio of material to liquid, and reflux time on the extraction rate of essential oil were investigated. The results showed that, it will be best when the dosage was 200 kg, the extraction time was 4 hours, the ratio of material to liquid was 1∶4, and the reflux time was 2 hours,which can come up to 3.19?.
    Development and nutritional value evaluation of the potato flour ham
    CAI Sha, MEI Xin, HE Jian-jun, SUI Yong, SHI Jian-bin, XIONG Tian, CHEN Xue-ling, FAN Chuan-hui, CAI Fang
    2020, 59(24):  151-155.  doi:10.14088/j.cnki.issn0439-8114.2020.24.035
    Asbtract ( 512 )   PDF (1636KB) ( 157 )  
    References | Related Articles | Metrics
    Taking potato flour as raw materials, using sensory evaluation and texture properties as the indicators,through single factor experiments and orthogonal design experiments,the factors affecting the texture properties and sensory evaluation of potato flour ham were optimized,such as lean meat percentage, mashed potato percentage, soy isolate protein percentage, water percentage. The results showed that, when the lean meat percentage was 96%, mashed potato percentage was 10%, soy isolate protein percentage was 6%, water percentage was 40%, the sensory evaluation of potato flour ham was the highest, which was 87.4. Compared with the commercial hams, potato flour ham had higher protein content and lower fat content, which was more suitable for all kinds of people.
    Detection Analysis
    Study on antioxidant activity and HPLC fingerprints from different polarity parts of Oxalis corniculata L.
    LI Xiao-na, JI Yong-hui, WANG Jing-long, ZHANG Li-hua, ZANG Yuan-long, YANG Bin, LIU Chun-he, LIU Chun-jiang
    2020, 59(24):  156-163.  doi:10.14088/j.cnki.issn0439-8114.2020.24.036
    Asbtract ( 396 )   PDF (1884KB) ( 171 )  
    References | Related Articles | Metrics
    The contents of polyphenol, flavonoids, polysaccharide in the bulb of Oxalis corniculata L. were measured by ferrous tartrate method, erinitrit-aluminum nitrate-sodium hydroxide method, phenol-sulfuric acid method and DNS method. Using acetonitrile and 0.1% phosphoric acid as mobile phase and gradient elution, the fingerprints of different polar parts of alcohol extract of Oxalis ferruginosa were investigated. The antioxidant activity in vitro was determined by 1,1-diphenyl-2-picrylhydrazine radical scavenging method and hydroxyl. OH radical scavenging method with ascorbic acid as positive control. The polyphenol contents of the two parts were 5.45 and 4.73 mg/g. The flavonoids contents of the two parts were 16.62 and 7.70 mg/g. The polysaccharide contents of the two parts were 17.00 and 121.02 mg/g. 32 peaks were calibrated on the basis of peak 21, and 31 inner cores were calibrated were set at 280 nm. The common peaks in different polar parts are the outer skin: petroleum ether phase 8, chloroform phase 22, ethyl acetate phase 22, n-butanol phase 26, water phase 9; inner core: petroleum ether phase 14, chloroform phase 21, ethyl acetate phase 17, n-butanol phase 19, water phase 10. In the determination system of antioxidant activity, the outer skin and inner core of bulb had certain antioxidant activity in different polar parts. The chloroform phase and ethyl acetate showed better antioxidant activity than the two free radicals. The contents of polyphenol, flavonoids and polysaccharide were rich in the bulb of Oxalis corniculata L.. After extraction by petroleum ether, chloroform, ethyl acetate and n-butanol, the chemical fingerprints and antioxidant ability of the ethanol extracts were different, which could provide reference for the discovery of effective substances in the later period.
    Study on rice-Cadmium of safety production threshold of cultivated land in high geological background area of Hubei province
    YI Tian, PENG Li-jun, CUI Wen-wen, WANG Ming-rui, YAO Jing-jing
    2020, 59(24):  164-168.  doi:10.14088/j.cnki.issn0439-8114.2020.24.037
    Asbtract ( 363 )   PDF (1720KB) ( 205 )  
    References | Related Articles | Metrics
    It is inconsistent to exceed the Cd standard in the high geological background area and the Cd standard in the rice area, so the safe production threshold of rice-cadmium cultivated land suitable for the high geological background area should be selected.This study based on the soil heavy metals-rice, physical and chemical means of correlation analysis, through spatial interpolation and parameter calculation, get the following results,Cd content in soil and soil physical and chemical indicators (pH, organic matter, cation exchange capacity) is weak, the linear relationship between the regions with high explain geological background due to factors such as soil background value, the soil pollution is serious.There is a close relationship between Cd in rice and soil physicochemical properties, which may be because although the background value of heavy metals in high geological background is high, it is not affected by human activities and has little impact on Cd content of rice planted.By calculating the required parameters and interpolation, the cultivated land safety threshold of the geological high background area was preliminarily judged to be 0.31 mg/kg. The reasonableness of the predicted results was tested, and the result showed that the cultivated land security threshold in the geological high background area was set reasonably.
    Biological Engineering
    Physiological response and transcriptome analysis of cotton leaf in the two-leaf period under low temperature
    WANG Xiao-man, WANG Qiong-shan, WANG Xiao-gang, ZHANG Jiao-hai, ZHANG You-chang, ZHANG Xing-zhong, BIE Shu, XIA Song-bo
    2020, 59(24):  169-176.  doi:10.14088/j.cnki.issn0439-8114.2020.24.038
    Asbtract ( 390 )   PDF (3166KB) ( 380 )  
    References | Related Articles | Metrics
    CN01 and SJB016 (a low temperature resistant cotton) were used as materials to study the effects of low temperature treatment on cotton in the two-leaf period. Firstly, We separately measured the contents of malondialdehyde (MDA), soluble sugar (SS) and proline (Pro) in CN01 and SJB016 at 25 ℃ (CK) and 12 ℃ (low temperature treatment) for 0, 3, 6, 12, 24, 48 and 72 h. It was found that the contents of MDA, SS, and Pro in CN01 and SJB016 increased after 12 ℃ treatment compared with 25 ℃. After low temperature treatment, the content of MDA in SJB016 increased less than CN01, the content of SS in SJB016 increased larger than CN01, and the content of Pro in SJB016 increased less than CN01. Transcriptome analysis of cotton leaves in two-leaf period after low temperature treatment for 6 hours showed that the photosynthesis-related genes were up-regulated in CN01 after 12 ℃ treatment, while the genes of photosystem II oxygen-releasing complexes (PsdP, PsdQ, et al) were down-regulated in SJB016, compared with 25 ℃. The up-regulated differentially expressed genes associated with phytohormone were more than down-regulated genes in CN01 and SJB016, and ethylene-responsive transcription factors ERF053, ERF020, and ERF061 significantly up-regulated. These genes are deemed to play an important role in improving the cold resistance of cotton.
    Isolation and identification of swine Mannheimia haemolytica
    LIU Ze-wen, YUAN Fang-yan, LIU Wei, GAO Ting, ZHOU Dan-na, YANG Ke-li, DUAN Zheng-ying, GUO Rui, LIANG Wan, TIAN Yong-xiang
    2020, 59(24):  177-178.  doi:10.14088/j.cnki.issn0439-8114.2020.24.039
    Asbtract ( 474 )   PDF (1775KB) ( 209 )  
    References | Related Articles | Metrics
    A gram negative bacilli was isolated from fattening pigs of a pig farm with pneumonia in Hubei province. After pathogen isolation, gram staining, PCR identification and biochemical identification, it was identified as Mannheimia haemolytica. The bacterium was gram-negative short bacilli with two poles stained. The results of sequence comparison showed that the homology rate of the gene sequence of the strain was more than 99.7%, and its biochemical characteristics accorded with the biochemical characteristics of the strain. The strain 2×108 CFU/mL can kill mice and has strong pathogenicity. The results of drug sensitivity test showed that the strain was sensitive to ampicillin, cefotaxime and ciprofloxacin.
    Economy & Management
    Research on the identification of entrepreneurial opportunities of the bottom groups in the process of urbanization
    HAN Lu, BAO Hai-jun, CHEN Meng-yuan
    2020, 59(24):  179-183.  doi:10.14088/j.cnki.issn0439-8114.2020.24.040
    Asbtract ( 369 )   PDF (1643KB) ( 149 )  
    References | Related Articles | Metrics
    Taking eight bottom groups in the urbanization process of Qingyang city of Gansu province as the typical case, using case study method, this study constructed the operation model of the identification of the entrepreneurial opportunities of the bottom groups based on the theoretical framework of the entrepreneurial opportunities identification, and analyzed the factors affecting the entrepreneurial opportunities identification of the bottom groups. The results showed that ,the identification of entrepreneurial opportunities of the bottom groups in the process of urbanization was mainly influenced by two factors: Individual characteristics and entrepreneurial environment, and the influencing factors in different stages were different. Finally, the corresponding countermeasures were put forward to improve the ability of the bottom groups to identify the entrepreneurial opportunities.
    Marketing strategy optimization of geographical indication agricultural products enterprises under the background of industry poverty alleviation:Take Chuju in Chuzhou city as an example
    SHI Lei
    2020, 59(24):  184-188.  doi:10.14088/j.cnki.issn0439-8114.2020.24.041
    Asbtract ( 465 )   PDF (1525KB) ( 485 )  
    References | Related Articles | Metrics
    Geographical indication agricultural products had distinct regional characteristics and competitive advantages, which was an effective way to realize rural industrial poverty alleviation and rural revitalization. This study selected Chuju, an agricultural product with geographical indication, from the perspective of product marketing, analyzed the problems existing in product marketing of Chuju enterprises under the background of industrial poverty alleviation, and put forward the optimization suggestions of marketing strategies, so as to help the regional industrial poverty alleviation and rural revitalization development.
    Research on optimization of agricultural products cold chain logistics distribution path based on customer satisfaction
    WEI Qing-hao, WU Xian
    2020, 59(24):  189-194.  doi:10.14088/j.cnki.issn0439-8114.2020.24.042
    Asbtract ( 466 )   PDF (2463KB) ( 298 )  
    References | Related Articles | Metrics
    The lack of cooperation between the traditional agricultural products cold chain logistics distribution stations in China not only caused waste of resources, but also lead to high time cost and low customer satisfaction. Based on the optimization of agricultural products cold chain logistics distribution path, this study established the collaborative distribution optimization model from the perspective of customer satisfaction, and verified the effectiveness of the model through case analysis. The results showed that, collaborative distribution can effectively reduce the distribution cost and improve the distribution efficiency, which provided the corresponding guidance for cold chain logistics enterprises to choose high-quality distribution path.
    Impact of COVID-19 on Wuhan residents' willingness of rural homestead land transfer
    LIU Zhi-ling, WANG Zhen-wei
    2020, 59(24):  195-198.  doi:10.14088/j.cnki.issn0439-8114.2020.24.043
    Asbtract ( 432 )   PDF (1565KB) ( 126 )  
    References | Related Articles | Metrics
    Stduy on residents' willingness of rural homestead land transfer can provide intellectual support to promote the pilot reform of the separation of “Theree Rights” in rural homestead land and furtherly to help realize rural revitalization strategy. This study employed regulatory focus theory to analyze the influence mechanism of COVID-19 on Wuhan residents' willingness of rural homestead land transfer. It compared Wuhan residents' willingness of rural homestead land transfer from Janauray 23rd to March 10th in 2020 with that in 2019,then the lag correlation analysis was conducted. The results showed that, Wuhan residents' willingness of rural homestead land transfer increased by 39.52 percent because of COVID-19. It had high positive correlation with the number of new confirmed cases, new deaths and cumulative deaths. The response of Wuhan residents' willingness of rural homestead land transfer to them lagged 13 or 14 days. Some suggestions were given to motivate Wuhan residents to transfer rural homestead land.
    Analysis on the characteristics of external cooperation of Xinjiang regional innovation system elements
    WU Zheng-ping, SHEN Gao-yan, LI Xiu
    2020, 59(24):  199-205.  doi:10.14088/j.cnki.issn0439-8114.2020.24.044
    Asbtract ( 379 )   PDF (1666KB) ( 267 )  
    References | Related Articles | Metrics
    The elements of regional innovation system in Xinjiang included universities, enterprises and scientific research institutions.Based on the data from 2013 to 2017, this study analyzed the characteristics of external cooperation of Xinjiang regional innovation system elements by analyzing the characteristics of core papers published by Xinjiang undergraduate colleges with external cooperation ,the characteristics of patents invented by Xinjiang main board listed companies with external cooperation, and the characteristics of Xinjiang technology flow region. The results showed that,① the regional distribution of core papers published by Xinjiang undergraduate colleges with external cooperation was relatively wide, and mainly in the eastern economically developed areas;② the number of patents invented by Xinjiang main board listed companies with external cooperation was less, and the technical cooperation between enterprises and external cooperation was less; ③ the distribution of Xinjiang technology flow region was mainly concentrated in the central and eastern areas. Finally, some countermeasures and suggestions were put forward to improve the technical cooperation between Xinjiang and external cooperation.
    Research on the legal obstacles and countermeasures of using collective construction land to build rental houses in Guangzhou city
    ZHOU Wei-ji, CHENG Xiao-man, WEN Han-feng, YE Jun-feng
    2020, 59(24):  206-209.  doi:10.14088/j.cnki.issn0439-8114.2020.24.045
    Asbtract ( 317 )   PDF (1486KB) ( 166 )  
    References | Related Articles | Metrics
    From the perspective of law and management, this study made a systematic analysis of the legal obstacles of using collective construction land to build rental houses in Guangzhou city through the methods of literature research and comprehensive analysis. The results showed that, there were some problems in the using collective construction land to build rental houses in Guangzhou city, such as the conflict with current laws and regulations, lack of legal basis, involving many stakeholders, fuzzy income distribution system and blocked development and financing channels. Therefore, it was suggested that the current laws and regulations should be amended timely, the property right system should be improved, the supervision mechanism should be established and perfected, the financing market should be stabilized, and the financial policy should be relaxed.
    Development potential of agricultural products trade between China and ASEAN under the background of “The Belt and Road”: Based on trade gravity model
    WANG Rui, YU Xin-xin, LIU Yu
    2020, 59(24):  210-214.  doi:10.14088/j.cnki.issn0439-8114.2020.24.046
    Asbtract ( 520 )   PDF (1543KB) ( 286 )  
    References | Related Articles | Metrics
    Based on the trade data of agricultural products between China and ASEAN from 2008 to 2017, this study analyzed the competitiveness and complementarity of bilateral trade of agricultural products between China and ASEAN by using the regional revealed comparative advantage index (RRCA) and trade complementarity index (TCI), and calculated the trade potential of agricultural products between China and ASEAN in 2013 and 2017 through the trade gravity model. On this basis,the development potential of agricultural products trade between China and ASEAN under the background of “The Belt and Road” was analyzed,and the countermeasures and suggestions to promote the development of bilateral trade of agricultural products between China and ASEAN were put forward.
    Analysis on the green production path of tea farmers in southern Shaanxi guided by tea geographical indications
    CHENG Dang-wei
    2020, 59(24):  215-218.  doi:10.14088/j.cnki.issn0439-8114.2020.24.047
    Asbtract ( 339 )   PDF (1496KB) ( 168 )  
    References | Related Articles | Metrics
    How to promote agricultural supply-side structural reform as the main line, change the mode of agricultural development, promote the transformation of farmers' production mode from conventional production to green production, and improve the quality and efficiency of agricultural products was an urgent task for the sustainable development of tea industry in southern Shaanxi. Based on the management of tea geographical indications in southern Shaanxi, this study explored the way to guide tea farmers' green production with tea geographical indications, improved the quality and production efficiency of tea geographical indications, promoted rural revitalization and sustainable development of tea industry, increased efficiency of tea industry and the income of tea farmers.
    Evaluation measure and analysis of agricultural science and technology popularization efficiency in Hubei province:Based on the survey of peasant household data in Hubei province
    ZHOU Yan, GUO Hong-xi, KE Yan-ruo, WU Cheng-cheng, XIA Juan, WANG Zhi-hong
    2020, 59(24):  219-225.  doi:10.14088/j.cnki.issn0439-8114.2020.24.048
    Asbtract ( 360 )   PDF (1569KB) ( 231 )  
    References | Related Articles | Metrics
    Agricultural science and technology extension service was an important means to improve agricultural production efficiency in China, which was the key to the green and efficient development of agriculture. In this paper, the data were obtained through the peasant survey in the sample area of Hubei Province, and the Ordinal Logistics model was used for econometric analysis to measure the evaluation of peasant households on the economic efficiency, social efficiency and ecological efficiency of agricultural science and technology extension service. First, the results showed that the field demonstration and household guidance were the most popular methods for farmers.Second, the ranking of farmers' approval attitude towards various benefits of agricultural science and technology extension service was: economic benefits > ecological benefits > social benefits. Third, when the other conditions remain unchanged, the two popularization methods of “observation and learning” and “exchange meeting” can significantly improve farmers' evaluation of the service effect, while “issuing technical guidance materials” was not conducive to the improvement of farmers' evaluation of the service effect of agricultural science and technology popularization. Finally, it was found that the government should improve the cultural and educational level of farmers in various aspects, strengthen the exemplary leading role of agricultural science and technology demonstration households, and increase policy support and fund input to improve the efficiency of agricultural science and technology popularization services.
    Rural Revitalization
    Strategies for high-quality development of rural tourism in Henan province under the strategy of rural revitalization
    WEI Yan, LI Bao-guo, YUAN Meng
    2020, 59(24):  226-229.  doi:10.14088/j.cnki.issn0439-8114.2020.24.049
    Asbtract ( 445 )   PDF (1492KB) ( 649 )  
    References | Related Articles | Metrics
    Rural tourism is an important part of rural revitalization, and the dialectical development relationship between them is mutual support and mutual promotion. This study analyzed the requirements for the high-quality development of rural tourism under the strategy of rural revitalization, expound the current situation and existing problems of rural tourism development in Henan province, and put forward the development strategies of scientific planning, innovation of rural tourism products, creation of rural tourism brands, improvement of supporting facilities, effective marketing, protection of rural ecology, and assistance of tourism poverty alleviation.
    Difficulties and enlightenment of the development of farmers' cooperatives in Xiaogan city
    WU Ya-ling, HUANG Wen-wen, LIU Hai-bo, LEI Shu-yan
    2020, 59(24):  230-232.  doi:10.14088/j.cnki.issn0439-8114.2020.24.050
    Asbtract ( 326 )   PDF (1480KB) ( 184 )  
    References | Related Articles | Metrics
    In recent years, Xiaogan city has strengthened the policy support, promoted the farmers' cooperatives to become bigger and stronger, promoted the standardized development of farmers' cooperatives, improved the service driving ability of farmers' cooperatives, and significantly improved the development quality of farmers' cooperatives. On the basis of summarizing the main measures for the development of farmers' cooperatives in Xiaogan city, in view of the main problems faced by the development of farmers' cooperatives in Xiaogan city, this study put forward some countermeasures and suggestions from the aspects of characteristic industry support, financial insurance docking, agricultural talent training, supporting facilities construction and so on.
    Exploration on the strategy of rural revitalization in Shaanxi based on the sustainable development of rural society
    LI Jing
    2020, 59(24):  233-235.  doi:10.14088/j.cnki.issn0439-8114.2020.24.051
    Asbtract ( 368 )   PDF (1474KB) ( 191 )  
    References | Related Articles | Metrics
    Excellent local culture was the inherent wealth of rural society and the foundation and power of sustainable development of rural society. This study expounded the foundation of Shaanxi rural revitalization development, analyzed the examples of Shaanxi excellent local culture helping rural revitalization development, discussed the opportunities and challenges faced by Shaanxi excellent local culture inheritance, and put forward suggestions on Shaanxi excellent local culture helping rural revitalization development, so as to drive the development of rural industry, and lay the cornerstone of effective governance of rural society.
    Study on the evolution of symbiotic behavior pattern of multi-agents in tourism community governance:Taking Huzhu Tu Nationality Homeland Park in Qinghai province as an example
    SHAO Li, TANG Zhong-xia, XIANG Cheng, REN Xi-xian, CHAI Jian
    2020, 59(24):  236-241.  doi:10.14088/j.cnki.issn0439-8114.2020.24.052
    Asbtract ( 402 )   PDF (1789KB) ( 440 )  
    References | Related Articles | Metrics
    Based on symbiosis theory, this study took Huzhu Tu Nationality Homeland Park in Qinghai province as the case, selected government, enterprises, residents and tourists as the symbiotic main units, analyzed and discussed the symbiotic behavior pattern and evolution of multi-agents in tourism community governance through two field survey data. The results showed that,①there was a positive symbiotic relationship between different governance agents in 2012 and 2017; ② there were differences in the interaction and influence between different governance agents in the two periods; ③compared with 2012, the symbiotic correlation among government-enterprises, government-tourists, residents-enterprises and residents- tourists in 2017 was strengthened, the symbiotic correlation between government-residents and enterprises-tourists in 2017 was weaken; ④the symbiotic behavior pattern has evolved from the R-G-E-T pattern in 2012 to the E-R-G-T pattern in 2017.