Effects of microecological preparation of fermented Chinese medicine on the intestinal morphology, gene expression and flora of weaned piglets

doi:10.14088/j.cnki.issn0439-8114.2022.16.026

Abstract

Abstract: A total of 120 piglets （Duroc×Landrace×Yorkshire） weaned at 23 days of age were randomly divided into four groups of control group （basal diet）, antibiotic group （basal diet + 0.075 g/kg chlortetracycline + 0.015 g/kg enramycin + 0.1 g/kg olaquindox）, test group Ⅰ （basal diet + 0.3% fermented Chinese medicine preparation + 0.15 g/kg Bacillus subtilis TL）, and test group Ⅱ （basal diet + 0.3% fermented Chinese medicine preparation + 0.3 g/kg Bacillus subtilis TL）. There were 30 piglets in each group, the experimental period was 30 days. The effects of fermented Chinese medicine microecological preparation on intestinal structure, intestinal tissue gene expression and intestinal flora under weaning stress were evaluated. The results showed that the villus length/crypt depth （VH/CD） of duodenum, jejunum and ileum in group Ⅰ was the highest, which was 32.27% （P<0.01）, 37.23% （P<0.01） and 56.23% （P<0.01） higher than that in control group, respectively. Compared with the control group, MUC2 gene expression was up-regulated in antibiotic group and test group Ⅰ, but the difference was not significant （P>0.05）. The number of E. coli in group Ⅰ was lower than that in the control group （P>0.05）. Compared with the control group, the number of Lactobacillus in group Ⅰ and group Ⅱ increased by 21.22% and 14.32%（P<0.05）, 33.70%（P<0.01） and 26.09%（P<0.05） respectively. Fermented Chinese herbal microecological preparation could increase the VH/CD value of duodenum, jejunum and ileum, promote the reproduction of Lactobacillus in cecum contents, and reduce the number of E.coli in cecum contents.

Key words: fermented Chinese medicine, microecological preparation, weaned piglets, intestinal morphology, intestinal gene expression, intestinal flora

CLC Number:

S858.28

HE Wei-min, KUANG Shi-chang, LI Xiao-wen, WANG Chun, CUI Wei-tao, WANG Xi-liang, XIAO Yun-cai, LI Zi-li, ZHOU Zu-tao. Effects of microecological preparation of fermented Chinese medicine on the intestinal morphology, gene expression and flora of weaned piglets[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(16): 137-141.

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