The expression of exogenous genes in mammalian cells by using baculovirus

doi:10.14088/j.cnki.issn0439-8114.2020.23.036

Abstract

Abstract: Baculovirus is a kind of virus that infects invertebrates,and they are widely used in scientific research and biomedicine as expression vector and gene transduction vector.Based on “Bac-to-Bac”operating system of baculovirus,we transformed baculovirus vector into a transduction vector with capable of expressing exogenous genes in mammalian cells in this study.First,the PH and p10 promoters of pFastBac-Dual plasmids in the Bac-to-Bac operating system were replaced with the CMV promoter and EF1 promoter.Then,the egfp gene and mCherry gene were inserted into the downstream of EF1 promoter and CMV promoter respectively.The transposition was performed to obtain the recombinant baculovirus genomeexpressing egfp and mCherry genes,and recombinant baculovirus was obtained by transfect the genome to Sf9 cells.The recombinant baculovirus can generate green and red fluorescence in insect cells and mammalian cells,indicating that the recombinant baculovirus constructed with CMV promoter and EF1 promoter can express exogenous genes in insect cells as well as mammalian cells through transduction.This vector can be used for eukaryotic expression of special proteins and research and development of gene therapy drugs.

Key words: baculovirus, gene expression, mammalian cells

CLC Number:

Q819

ZOU Gui-lian, LUO Qing-ping, SHAO Hua-bin, WEN Guo-yuan, YANG Ye, SHANG Yu. The expression of exogenous genes in mammalian cells by using baculovirus[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(23): 159-163.

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