Microspore culture and double haploid seed setting analysis in three rapeseed F1 hybrids

doi:10.14088/j.cnki.issn0439-8114.2020.01.007

Abstract

Abstract: To investigate the ploidy levels of microspore-derived plants and effect of colchicine and trifluralin treatment on regeneration, double haploid, and seed setting of regenerated plants in oilseed rape （Brassica napus L.）, the effects of microspore culture treatment with antimitotic agents colchicine and trifluralin on the frequency of embryo formation, embryo development, plant regeneration and diploidization rate in three F₁ hybrids of rapeseed cultivars were compared, and the ploidy levels of young plantlets were measured by flow-cytometry （FCM）. The results indicated three F₁ combinations obtained the most number of regenerated plantlets after treatment with 85 mg/L colchicine. The mean rates of DH plants from colchicine（85 mg/L） were higher than the other colchicine concentration, and maximum DH plants were derived from the trifluralin treatment（10 μmol/L）. After 4 ℃ cold treatment with embryos derived from three F₁ hybrid combination for 10 days, the number of embryos to plants conversion （EPC） was significantly increased. The fruity characters of DH population obtained by natural doubling and doubling agent of three F₁ combinations were investigated, and the results showed that the average pod length,pod number and 1 000-grain weight of double haploid plants treated from antimitotic agents was similar to the spontaneous double haploid plants.

Key words: rapeseed （Brassica napus L.）, microspore culture, colchicine, trifluralin, ploidy analysis, seed setting characters

CLC Number:

S565.4

WAN Li-li, WANG Zhuan-rong, YANG Guang-sheng, XIN Qiang, HONG Deng-feng, SUN Yu-hong, GAO Hong-xia. Microspore culture and double haploid seed setting analysis in three rapeseed F₁ hybrids[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 32-38.

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Microspore culture and double haploid seed setting analysis in three rapeseed F₁ hybrids

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