Abstract: To establish a GC-MS method and a scientific uncertainty evaluation model for the determination of tetramine residues in food poisoning, and to improve the quality control level of detection. The results showed that the linear relationship between the concentration of tetramine 25.0~250.0 ng/mL was good, Y=1.181×10³X+5.203×10³（R²=0.995 3）. The average recovery of three levels was 96.57%, and the corresponding RSD was 2.7%. The detection limit of the method was 0.098 μg/kg. When the content of tetramine in food poisoning was 0.186 7 mg/kg, the expanded uncertainty was 0.000 21 mg/kg. The method of determination of tetramine residues in food poisoning was confirmed, and the reference basis was provided for the evaluation of uncertainty in the determination of tetramine by GC-MS/MS.

Key words: GC-MS/MS, tetramine, uncertainty