Bioinformatics analysis of AkMYB5gene from Actinidia kolomikta （Maxim. et Rupr.）, Maxim

doi:10.14088/j.cnki.issn0439-8114.2020.11.028

Abstract

Abstract: To obtain information about AkMYB5 gene from Actinidia kolomikta （Maxim. et Rupr.） Maxim., the bioinformatics methods and tools were used to analysis the physical and chemical properties, hydrophilicity/hydrophobicity, transmenbranne domain, structure and function domain , secondary structure, tertiary structure, protein modification sites and homology of MYB5 from Actinidia kolomikta. The results showed that the length of AkMYB5 cDNA was 1 339 bp and the coding area was 1 053 bp coding 351 amino acid. AkMYB5 was a unstable and hydrophilic protein belonging to the MYB5 superfamily （PLN03212）. The analysis also demonstrated that AkMYB5 main component was Alpha-helix and random coil. Compaired to MYBs of the other 18 plants, AkMYB5 had near relationships with Actinidia chinensis var. chinensis.

Key words: Actinidia kolomikta （Maxim. et Rupr.） Maxim., AkMYB5, bioinformatics

CLC Number:

S663.4
Q75

LIU Dan, LI Ran-hong, CHEN Xin, WANG Li-feng. Bioinformatics analysis of AkMYB5gene from Actinidia kolomikta （Maxim. et Rupr.）, Maxim[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(11): 134-137.

References 10

[1]	陈鑫, 袁頔, 姜明, 等. 榆黄蘑菌糠提取液对盐胁迫下狗枣猕猴桃幼苗的影响[J]. 北方园艺, 2018(2):68-72.
[2]	王振兴, 曹建冉, 秦红艳, 等. 狗枣猕猴桃彩叶色素含量和结构共同影响叶色[J]. 植物生理学报, 2016, 52(12):1921-1926.
[3]	李然红, 金志民, 陈鑫, 等. 狗枣猕猴桃研究进展[J]. 中国林副特产, 2015(2):84-85.
[4]	CHEN B J, YONG W, HU Y L, et al.Cloning and characterization of a drought-inducible MYB gene from Boea crassifolia[J]. Plant science, 2005, 168(2):493-500.
[5]	PAZ-ARES J, GHOSAL D, WIENAND U, et al.The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators[J]. EMBO J, 1987, 6(12): 3553-3558.
[6]	刘蕾, 杜海, 唐晓凤. MYB转录因子在植物抗逆胁迫中的作用及其分子机理[J]. 遗传, 2008, 30(10):1265-1271.
[7]	乔孟, 于延冲, 凤宁. 拟南芥R2R3-MYB类转录因子在环境胁迫中的作用[J]. 生命科学, 2009, 21(1):145-150.
[8]	COMINELLI E, SALA T, CALVI D, et al.Overexpressions of the Arabidopsis AtMYB41 gene alter cell expanision and leaf surface permeability[J]. Plant journal, 2008, 53(1):53-64.
[9]	陈江, 唐小慧, 任超翔, 等. 红花转录因子广泛参与调控黄酮类成分的合成[J]. 药学学报, 2018, 53(1):141-146.
[10]	李军, 赵爱春, 刘长英, 等. 桑树花青素合成相关MYB类转录因子的鉴定与功能分析[J]. 西北植物学报, 2016, 36(6): 1110-1116.

[1]	ZHAO Yong-yang, CHEN Wei-jia, DING Jing-feng, AO Yi-heng, FENG Ao, ZHOU Jia-yu. Genome wide analysis of the phenylalanine ammonia lyase （PAL） gene family from Senna tora [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(6): 181-186.
[2]	WANG Guo-hong, LI Dan-lin, ZHU Bo-run, MA Huai-liang, MU Yan, LYU Feng-xia, JIANG Ming. Cloning and bioinformatics analysis of dopachrome tautomerase gene from Auricularia auricula [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(6): 153-156.
[3]	LIAN Ming-dong, CHEN Hong, YANG Meng-ping, TIAN Xin-min. Bioinformatics analysis of NADH dehydrogenase subunit 1 （ND1） gene in Pteromys volans [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(3): 161-164.
[4]	LIU Fang, ZHANG Zhi-gang, FANG Wei, WANG Kai-mei, WANG Yue-ying. Bioinformatics analysis of heat-shock protein Hsp70 from Spodoptera frugiperda [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 153-159.
[5]	LI Jie-qiong, LI Xiao-wan. Bioinformatics prediction of Microdera punctipennis Attacin genes structure and function [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 170-176.
[6]	CHEN Wei, LIU Xiao-yan, ZHU Lei. Bioinformatics analysis of LuxR family regulatory proteins of sulfate-reducing bacteria class [J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 177-184.
[7]	ZHU Bin, WANG Xiao-shuang, LUO Xi, HU Xin. Bioinformatics analysis of CRM gene family in Brassica rapa [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(9): 141-146.
[8]	GUO Shuai, YANG Juan, LIU Shi-yu, YANG Feng-li, XIONG Qi, WANG Zhao-xiong. Polymorphism and bioinformatics analysis of MC4R gene in Macheng black goat [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(21): 140-145.
[9]	CHEN Zhen, HE Xiao-ying, WANG Jing-qiao, LI Gen-ze, SHU Zheng-qi, ZHAO Kai-qin, CHENG Xiao-mao, ZU Feng. Bioinformatics analysis of BnFAD2 gene in Brassica napus L. [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 134-140.
[10]	LIN Biao-sheng, ZHANG Huan-xing, HE Yu-qin, LUO Mao-chun, LIN Zhan-xi. Cloning and bioinformatics analysis on nifH gene of endophytic nitrogen-fixing bacteria Klebsiella variicola GN02 isolated from Pennisetum giganteum [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(3): 147-153.
[11]	AN Yu-ting, LI Ran-hong, CHEN Xin, LIU Dan. Bioinformatics analysis of BpPR1 gene from Betula platyphylla [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(12): 171-174.
[12]	ZHANG Shuang, XU Yong, TONG Lin, ZHANG Zhong-dong, YE Hong. Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma [J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 156-158.
[13]	HAO Ai-ping, GUO Hui-yan, QI Hong-ling, WEI Ji-cheng. Bioinformatic Analysis of GGPPS Protein in 15 Medicinal Plants [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(2): 131-136.
[14]	XUE Ju-kun, WANG Bo, WANG Lian-ping, YANG Chun-yu, GUO Hui-yan, HAO Ai-ping. Moleculer evelution of miR-398-3p gene family and prediction of their target gene [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(14): 141-147.
[15]	LU Yan-yan, WANG Chao, SHI Fu-mei. Research advances on protein interaction technology [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 5-10.