Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma

doi:10.14088/j.cnki.issn0439-8114.2020.01.036

Abstract

Abstract: The amino acid sequence of the yeast extract of Astaxanthin was used as the research object. The physical and chemical properties and subcellular localization were determined by online prediction tools such as ProtParam, SignalP, PSORT, ProtScale, SOMPA, PredictProtein, PROSITE, COILS and SWISS-MODEL., pro-hydrophobicity, secondary structure, tertiary structure, etc. were analyzed. The invertase consisted of 557 amino acid residues and was a signal-free peptide, a transmembrane structure, and a soluble hydrophilic protein localized in cytochrome oxidase. The alpha helix and random coil are the primary secondary structural elements. The tertiary structure uses the B-chain of PDB ID 5vcc.1 as a template, and its function is predicted to be cytochrome P450. The bioinformatics method is more reliable in analyzing and predicting the results, which lays a theoretical foundation for further research on the biosynthesis regulation mechanism of astaxanthin.

Key words: astaxanthin, phaffia rhodozyma, β-carotene converting enzyme, bioinformatics

CLC Number:

S963.73

ZHANG Shuang, XU Yong, TONG Lin, ZHANG Zhong-dong, YE Hong. Bioinformatics analysis of β-carotene converting enzyme （Asy） gene from phaffia rhodozyma[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(1): 156-158.

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