Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses

doi:10.14088/j.cnki.issn0439-8114.2020.16.026

Abstract

Abstract: Coagulase-negative staphylococci （CoNS） are a group of opportunistic pathogens, which are widely spread in the environment. Animal breeding is an important source of pathogen spreading. However, the prevalence and characteristics of CoNS in the bioaerosols of poultry houses are unclear. In this study, through further isolation and identification of bacteria collected from bioaerosols in poultry farms, 102 strains of coagulase-negative staphylococci were collected. Among these isolates, 41.18% isolates were Staphylococcus sciuri, which was the predominant species, followed by S. equorum （21.57%）, S. saprophyticus （18.63%）, S. haemolyticus （10.78%） and S. xylosus （5.88%）. Resistance analysis showed that high resistance rates to penicillin, ampicillin, ciprofloxacin, chloramphenicol, erythromycin, tetracycline, clindamycin and oxacillin among these staphylococci. The presence of mecA was screened by PCR in all CoNS, and it showed that 36.3% isolates contained resistance gene mecA and were resistance to oxacillin, and mecA is a marker resistance gene of methicillin-resistant S. aureus （MRSA）.

Key words: poultry houses, bioaerosols, CoNS, drug-resistance, mecA

CLC Number:

S858.31

LU Yuan-qing, ZHANG Teng-fei, SHAO Hua-bin, ZENG Chi. Isolation, identification and drug resistance analysis of Staphylococci in the air of chicken houses[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(16): 118-121.

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