Construction of the thermostable xylanase whole-cell biocatalyst based on yeast cell surface display technology and enzymatic analyses

doi:10.14088/j.cnki.issn0439-8114.2021.07.024

Abstract

Abstract: Xylanase is an important industrial enzyme with great potential for applications in feed, paper, textiles, food and other fields. In this study, the xylanase DSB was displayed on the cell surface of pichia pastoris using the cell wall protein Gcw61 as an anchor protein. The highest activity of DSB whole-cell biocatalyst reached 13 510 U/g by shake-flask fermentation. Then the enzymatic properties of DSB whole-cell biocatalyst and free enzyme were determined. The results showed that the optimum reaction temperature of both enzymes was 65 ℃ and the optimum reaction pH was 6.5. But the thermal stability of DSB whole-cell biocatalyst was better than free xylanase under high temperature condition 70 ℃ and acidic condition pH 3.0. In addition, the DSB whole-cell biocatalyst could maintain high enzyme activity and was more stable than free xylanase under high concentration of NaCl, methanol and various metal ions （Na⁺, Ca²⁺, Ni²⁺）.

Key words: xylanase, whole-cell biocatalyst, pichia pastoris, enzymatic properties

CLC Number:

Q814.9

DENG Xiang-lian, LIU Qing-yi, XIONG Hai-rong, ZHANG Li. Construction of the thermostable xylanase whole-cell biocatalyst based on yeast cell surface display technology and enzymatic analyses[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(7): 120-125.

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