HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (18): 115-120.doi: 10.14088/j.cnki.issn0439-8114.2019.18.028

• Detection Analysis • Previous Articles     Next Articles

Comparative test of enzyme reagent method for rapid detection of organophosphorus pesticide residues

LUO Jun-xia1, ZHAO Jian-bo2, JIA Dong1, SHANG Hang-ying1, HAN Luo-li1, SONG Xiao-shuang1   

  1. 1.Zhengzhou Agricultural Product Quality Inspection and Circulation Center,Zhengzhou 450006,China;
    2.Zhengzhou Agricultural Technology Promotion Center,Zhengzhou 450006,China
  • Received:2019-01-12 Online:2019-09-25 Published:2019-11-08

Abstract: Four organophosphorus pesticides, including dichlorvos, quinthion, trichlorfon and octamphos-methyl, were rapidly determined by six reagent kits of commercial enzyme inhibition. The results showed that the reproducibility of pesticide residue in the rapid detection kit by enzyme inhibition decreased with the increase of inhibition rate. The sensitivity and detection limit of each reagent box to several pesticides were different. The inhibition rate of each reagent box for the four pesticides is not the same as the linear regression range of the logarithm of the pesticide concentration. There was little difference between 3.0 mL extraction and 2.5 mL extraction during the test, and individual pesticides differed at individual concentration levels. Since the reagent box for rapid detection of pesticide residues by enzyme inhibition method has different sensitivity for the detection of various pesticides, it is recommended that the sample with a suppression rate of ≥10% be reexamined in the actual test work, and that the chromatographic mass spectrometry be used for qualitative quantification.

Key words: enzyme inhibition method, rapid detection, organophosphorus pesticide, sensitivit

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