Isolation and Identification of Fusarium solani from Polygonatum kingianum

doi:10.14088/j.cnki.issn0439-8114.2019.03.018

HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (3): 65-67.doi: 10.14088/j.cnki.issn0439-8114.2019.03.018

• Plant Protection • Previous Articles Next Articles

Isolation and Identification of Fusarium solani from Polygonatum kingianum

YANG Lin-yi, CEHN Ze-li, LAI Qing-yu, CEHN Lu, SUN Yan, TANG Zhao-hui², ZHAO Ming-fu, WEN Guo-song

1a.Key Laboratory of Ministry of Education Agriculture Biodiversity for Plant Disease Management;1b.College of Agronomy and Biotechnology, Yunnan Agricultural University,Kunming 650021,China;
2.Malong County Hongxing Breeding Farmers’ Professional Cooperatives,Qujing 655000,Yunan,China

Received:2018-08-02 Online:2019-02-10 Published:2019-11-29

Abstract

Abstract: A pathogen from Polygonatum kingianum was isolated by pathogen isolation method, and identification of the pathogen was carried out by Koch's rule, morphological observation and ITS sequence analysis. The results show that the single colony mycelium of the pathogenic strain is white, the back is light yellow, and the mycelium is flocculent and flourishes. The conidia of the pathogenic strain have two forms, the large conidia are sickle-shaped or columnar, and the small conidia are oval or kidney-shaped. The pathogen was identified as Fusarium solani from the morphological, pathogenicity and ITS sequence analysis of the strains.

Key words: Polygonatum kingianum, Fusarium solani, isolation and identification

CLC Number:

R931

YANG Lin-yi, CEHN Ze-li, LAI Qing-yu, CEHN Lu, SUN Yan, TANG Zhao-hui, ZHAO Ming-fu, WEN Guo-song. Isolation and Identification of Fusarium solani from Polygonatum kingianum[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(3): 65-67.

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Isolation and Identification of Fusarium solani from Polygonatum kingianum

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