Study on hybrid embryo rescue technology of cabbage and Brassica napus

doi:10.14088/j.cnki.issn0439-8114.2022.13.013

Abstract

Abstract: In order to restore the fertility of male sterile materials of kale（Brassica oleracea var. capitata L.）, the male sterile line of kale GL16047 was used as the female parent and the Brassica napus N18008 was used as the male parent for crossing, and the ovaries and ovules were isolated and cultured after crossing to screen the suitable sampling time and culture medium for hybrid embryo salvage culture of kale and Brassica napus. The results showed that the time of isolation after pollination had a great influence on the development of hybrid embryos, and sampling too early or too late had an effect on the ovary embryo rate. On the 5th day after pollination, samples were taken for isolated cohort culture, the ovary growth was small, and the number of embryos was low. On the 10th day after pollination, samples were taken for isolated cohort culture. With the prolongation of post-pollination time, the influence of the uncoordinated metabolism of the hybrid nucleus and egg cell on the development of hybrid embryo increases, and the number of embryos emerging decreases; the embryo age determines the embryo rate. If the seed embryo is too small, it is difficult to culture in vitro after being separated from the mother. The shortest time for embryo survival is the 16th day after pollination. If sampling exceeds 24 days, the introduction of heterologous genetic material at the later stage of fertilization will cause genetic incongruity in the ovule, embryos are prone to abortion, and the embryo rate will decrease. The two hormones, NAA and 6-BA, play a very good role in promoting the development and formation of the isolated ovary and ovule. At the same concentration of NAA, 1.0 mg/L 6-BA was the best for ovary culture, and 5.0 mg/L 6-BA was the best for ovule culture. The 8th day after pollination is the suitable sampling time for ovary culture, and the 20th day after pollination is the suitable sampling time for ovule culture. MS+1.0 mg/L 6-BA+1.0 mg/L NAA+1.0 mg/L KT+activated carbon 1 000 mg/L medium is suitable for ovary culture; MS+5.0 mg/L 6-BA+1.0 mg/L NAA+1.0 mg/L KT + activated carbon 1 000 mg/L medium is suitable for ovule culture.

Key words: kale（Brassica oleracea L.）, male sterility, Brassica napus, embryo rescue

CLC Number:

S635

YANG Hong-li, XU Xue-zhong, HU Jing-feng, LAN Mei, ZHANG Li-qin, HE Jiang-ming, SONG Shuang. Study on hybrid embryo rescue technology of cabbage and Brassica napus[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(13): 65-68.

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