Analysis of endophytic fungi and the rhizosphere soil fungi communities of Polygonatum cyrtonema Hua.

doi:10.14088/j.cnki.issn0439-8114.2023.08.00

Abstract

Abstract: The community composition and diversity of endophytic fungi and rhizosphere soil fungi of wild Polygonatum cyrtonema Hua. from Hunan Province, Guizhou Province and Guangxi Zhuang Autonomous Region was analyzed based on Illumina MiSeq high-throughput sequencing technology. A total of 3 188 ASVs were obtained in all samples, and were divided into 11 phyla, 33 classes, 85 orders, 166 families, 320 genera and 375 species. Fungi mainly belonged to Ascomycota and Basidiomycota. At the genus level, the dominant genera of rhizosphere fungi were Cladophialophora and Exophiala in Guizhou Province, Archaeorhizomyces and Mortierella in Hunan Province, and Fusarium and Saitozyma in Guangxi Zhuang Autonomous Region; the dominant genera of endophytic fungi were Exosporium, Entoloma, etc. in Guizhou Province, while they were Geastrum, Mortierella, etc. in Hunan Province, and they were Fusarium, Saitozyma, etc. in Guangxi Zhuang Autonomous Region. The alpha diversity analysis showed that the diversity and richness of endophytic fungi and rhizosphere soil fungi communities in the medicinal parts of Polygonatum cyrtonema Hua. from these three provinces（region） were significantly different. The results of PCoA and UPGMA clustering showed that the composition of fungal communities in three areas was quite different; PICRUSt 2 predictive analytics showed that endophytic fungi and rhizosphere soil fungi were involved in multiple metabolic pathways and the production of active substances such as polysaccharides of Polygonatum cyrtonema Hua.

Key words: Polygonatum cyrtonema Hua., endophytic fungi, rhizosphere soil, high-throughput sequencing

CLC Number:

LI Ming-jian, CHEN Xiao-fang, CHENG Zhong-jun, REN Xiu-xiu, ZHANG Xiang-yu. Analysis of endophytic fungi and the rhizosphere soil fungi communities of Polygonatum cyrtonema Hua.[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(8): 13-20.

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