Changes of physiological indexes and expression of stress resistance related genes in ramie under drought stress

doi:10.14088/j.cnki.issn0439-8114.2024.05.002

Abstract

Abstract: To study the effects of drought stress on physiological characteristics and stress resistance related genes of ramie （Boehmeria nivea L.）, the physiological indexes, stress resistance related gene expression and agronomic traits of Huazhu No. 4 were measured by natural drought stress. The results showed that, compared with the control, the chlorophyll SPAD of ramie leaves decreased under drought stress, the relative conductivity, free proline content, malondialdehyde content and soluble sugar content of stem tip and leaves increased significantly （P<0.05）, the activities of superoxide dismutase （SOD）, catalase （CAT） and peroxidase （POD） increased, and the relative expression levels of stress-related genes BnP5CS1, BnWRKY1, BnACO1, Bn-α-amylase, BnAPX1, BnbZIP1, BnDREB19 and BnGR1 were increased. The relative expressions of BnP5CS1 and BnWRKY1 in leaves were increased 5.1 and 5.3 times compared with control. Compared with the control, the plant height, stem diameter, skin thickness, fresh aboveground weight and raw hemp weight per plant under drought stress were significantly decreased （P<0.05）, the stem diameter and skin thickness were decreased by 0.13 cm and 0.14 mm, respectively, and the fresh aboveground weight and raw hemp weight per plant were decreased by 52.05% and 39.53%, respectively.

Key words: ramie（Boehmeria nivea L.）, drought stress, physiological indexes, stress resistance gene, relative expression

CLC Number:

S563.1

ZHANG Ao-shen, XU Min, CHEN Lian, XIONG Wei, CHENG Chang-song, WANG Hong-wu. Changes of physiological indexes and expression of stress resistance related genes in ramie under drought stress[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 6-11.

References

[1] 王昕慧, 全芮萍, 刘婕仪, 等.苎麻种质资源遗传多样性分析研究进展[J].作物研究, 2021, 35(3): 282-286.
[2] CRAMER G R, URANO K, DELROT S, et al.Effects of abiotic stress on plants: A systems biology perspective[J]. BMC plant biology, 2011, 11(1): 163.
[3] 赵浩含,朱爱国,陈继康.农业抗旱策略及苎麻相关研究建议[J].中国麻业科学, 2022, 44(5): 285-292.
[4] GAUT B S, DIEZ C M, MORRELL P L.Genomics and the contrasting dynamics of annual and perennial domestication[J]. Trends in genetcis, 2015, 31(12): 709-719.
[5] 李林林, 马渊博, 张浪, 等. 3个苎麻品种对干旱胁迫的生理生化响应[J].中国麻业科学, 2018, 40(1): 22-27.
[6] 高润梅,石晓东,王林,等.当年生华北落叶松幼苗的耐旱性[J].林业科学, 2015, 51(7): 148-156.
[7] BAJGUZ A, HAYAT S.Effects of brassinosteroids on the plant responses to environmental stresses[J]. Plant physiology and biochemistry, 2009, 47(1): 1-8.
[8] 黄承建, 赵思毅, 王龙昌, 等.干旱胁迫对杂交苎麻脯氨酸积累、膜脂过氧化及抗氧化酶活性的影响[J].中国麻业科学, 2013, 35(2): 57-62.
[9] 马渊博. 水分胁迫对苎麻生长及生理特性的影响[D].长沙: 湖南农业大学, 2014.
[10] 杨帆, 苗灵凤, 胥晓, 等.植物对干旱胁迫的响应研究进展[J].应用与环境生物学报, 2007, 13(4): 586-591.
[11] 杨媛. 不同时期干旱对苎麻生理生化及产量品质的影响[D].武汉:华中农业大学, 2018.
[12] ANJUM S A, XIE X Y, WANG L C, et al.Morphological, physiological and biochemical responses of plants to drought stress[J]. African journal of agricultural research, 2011, 6(9): 2026-2032.
[13] 李葵花, 高玉亮, 吴京姬.转P5CS基因马铃薯“东农303”耐盐、抗旱性研究[J].江苏农业科学, 2014, 42(11):131-133.
[14] 李健, 杜成忠, 王露蓉, 等.甘蔗△~1-吡咯啉-5-羧酸合成酶基因转化烟草的抗旱性分析[J]. 华中农业大学学报, 2018, 37(2): 34-42.
[15] 吴华玲, 倪中福, 姚颖垠, 等. 15个普通小麦WRKY基因的克隆与表达分析[J].自然科学进展, 2008, 18(4): 378-388.
[16] SONG Y, CHEN L G, ZHANG L P, et al.Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis[J]. Journal of biosciences, 2010, 35(3): 459-471.
[17] 余建, 刘长英, 赵爱春, 等.桑树1-氨基环丙烷-1-羧酸氧化酶基因(MnACO)启动子功能分析[J].作物学报,2017, 43(6): 839-848.
[18] 黄建斌, 周文杰, 房磊, 等. ACC氧化酶基因AhACOs对花生耐盐性的影响[J].生物工程学报, 2023, 39(2): 603-613.
[19] 余伟林, 钟英丽, 揭雨成, 等.苎麻α-amylase基因的克隆与表达[J].农业生物技术学报, 2014, 22(1): 27-36.
[20] 朱守晶,周精华,揭雨成,等.苎麻抗坏血酸过氧化物酶基因的克隆和表达分析[J].植物遗传资源学报,2013,14(5):879-884.
[21] 汪溢. 干旱胁迫下AcAPX对拟南芥根形态建成的影响[D].合肥:安徽农业大学, 2021.
[22] 周精华,揭雨成,邢虎成,等.苎麻BnbZIP1转录因子基因的克隆与表达特征分析[J].中国农业科学,2013,46(7):1314-1322.
[23] 胡亚杰,王闯,刘卫群,等.干旱胁迫对转BnDREB1-5烟草组织结构和光合日变化的影响[J].江苏农业科学,2011,39(5):109-112.
[24] 宋贵方,樊伟丽,王俊娟,等.陆地棉干旱胁迫响应基因GhGR的克隆及特征分析[J].中国农业科学, 2012, 45(8): 1644-1652.
[25] 杨瑞芳, 王继龙, 佘玮, 等.水分胁迫对不同基因型苎麻生长发育的影响[J].现代农业科技, 2018(22): 33-35,37.