The pharmacognosy and UPLC fingerprint of the leaves of Camellia nitidissima var. phaeopubisperma

doi:10.14088/j.cnki.issn0439-8114.2024.05.016

Abstract

Abstract: The plant morphology, tissue structure and powder characteristics of Camellia nitidissima var. phaeopubisperma were observed by means of character identification and microscopic identification. The liquid fingerprint of Camellia nitidissima var. phaeopubisperma was established by ultra high-performance liquid chromatography （UPLC） and similarity evaluation system of traditional Chinese medicine chromatographic fingerprint. Principal component analysis and orthogonal partial least squares discriminant analysis were performed on the chemical composition by SIMCA-P14.1 software. The results of character identification showed that Camellia nitidissima var. phaeopubisperma was elliptical, oblong, rectangular or oval, thin leathery, hairless on both sides, reticulated leaf veins and serrulate or serrate on the margins. In the mesophyll tissue were present large branching irregular stone cells and clusters of calcium oxalate crystals; the vessels were annular and spiral, and the stomata were anomocytic type. The HPLC fingerprints indicated differences in the chemical composition of the tested Camellia nitidissima var. phaeopubisperma samples. Based on these differences, the samples could be divided into two categories using principal component analysis and orthogonal partial least squares-discriminant analysis, with vitamin B₆, phenylephrine, rutin, caffeine, and hyperoside being the key signature ingredients that distinguished the two types of fingerprints.

Key words: Camellia nitidissima var. phaeopubisperma, morphological identification, microscopic identification, ultra-high-performance liquid chromatography（UPLC） fingerprint, pharmacognosy

CLC Number:

YAO Han-ya, PAN Yong, GAO Hui, BAI Yan-yuan, LI Yao-yan, YAN Guo-yue, XIE Yang-jiao. The pharmacognosy and UPLC fingerprint of the leaves of Camellia nitidissima var. phaeopubisperma[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(5): 91-97.

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