Genetic toxicity of hair coloring creams to root tip cells of Vicia faba L.

doi:10.14088/j.cnki.issn0439-8114.2019.11.015

Abstract

Abstract: In order to study the genotoxicity of different kinds of hair coloring creams on root tip cells, the micronucleus test technique on the root tip cells of Vicia faba L. was used to investigate the genotoxicity of 3 different kinds of hair coloring creams（T, S and H） of varied concentrations. Micronucleus rate, chromosome aberration rate and pollution indexes were calculated. The results showed that the micronucleus rate of 3 kinds of hair coloring creams were both first increased and then decreased along with the increasing of the treated concentration. The dye concentrations of the peak number of micronuclei of 3 kinds of coloring creams was 0.10 g/L （T）, 1.00 g/L （S） and 4.00 g/L （H）. The 3 kinds of coloring creams had a certain genotoxicity under certain concentration ranges. And the chromosome aberration rate was generally positively correlated with micronucleus rate.

Key words: hair coloring creams, Vicia faba L., root tip cells, micronucleus, chromosome aberration, genotoxicity

CLC Number:

XU Yuan-yuan, PENG Xiao-ya, SONG Wen-zhen, TANG Hong-feng, PENG Yan-qiao, YUAN Xi, LI Min. Genetic toxicity of hair coloring creams to root tip cells of Vicia faba L.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(11): 67-69.

References

[1] 杨艳伟,刘思然,张卓娜.染发类产品中染发剂使用现状调查[J].中国卫生检验杂志,2017,27(6):899-901.
[2] 周永贵,李真观.染发剂的致突变、致癌和毒代动力学的研究进展[J].环境与职业医学,2008,25(1):23-28.
[3] 杨兆弘. 染发剂的毒性及安全监管[J].当代化工,2012,41(11):1246-1249.
[4] SHAHIN M,CHOP Y C,LEGUESNE N.Comparisons of mutation induction by six monocyclic aromatic amines in Salmonella typhimurium tester strains TA97,TA1537,and TA1538[J].Environ mutagen,1985,7(4):535-546.
[5] BOLT H M,GOLKA K.The debate on carcinogenicity of permanent hair dyes:New insights[J].Critical reviews intoxicology,2007,37(6):521-536.
[6] DE SANJOSE S,BENAVENTE Y,NIETERS A,et al.Association between personal use of hair dyes and lymphoid neoplasms in Europe[J].American journal epidemiology,2006,164(1):47-55.
[7] BAAN R,STRAIF K,GROSSE Y,et al.Carcinogenicity of some aromatic amines,organic dyes,and related exposures[J].Lancet oncol,2008,9(4):322-323.
[8] 李星彩. 染发剂烫发剂中的化学成分及其对人体的危害[J].微量元素与健康研究,2006,23(1):47-48.
[9] 孙波,姚孝元,王艳,等.欧盟化妆品卫生标准追踪[J].中国卫生检验杂志,2008,18(3):573-576.
[10] 李莹,周细平,郑艺维.市售染发剂对蚕豆根尖细胞的遗传损伤效应[J].福建农业学报,2017,32(7):779-783.
[11] 朱鸿雁,李睿,周谡,等.氧化型与非氧化型染发剂遗传毒性检测的试验研究[J].第六届全国药物毒理学年会论文集[C].重庆:上海市食品药品检验所,2016.527-528.
[12] GRANT W F.Higher plant assays for the detection of chromosomal aberratians and monitoring environmental chemicals[J].Mutation research,1999,426(2):107-112.
[13] 国家环境保护局.环境检测技术规范部分[M].北京:中国环境科学出版社,1986.75-78.
[14] 段昌群,王焕校.重金属对蚕豆的细胞遗传学毒理作用和对蚕豆根尖微核技术的探讨[J].植物学报,1995,37(1):14-24.
[15] 钱晓薇. 重铬酸钾对蚕豆根尖细胞致畸效应的研究[J].遗传,2004,26(3):337-342.
[16] 李宏,黄晓天.表面活性剂诱发的蚕豆根尖细胞微核及染色体畸变[J].环境与健康杂志,2005,22(5):351-352.
[17] 张燕. Cr⁶+对蚕豆根尖细胞的遗传毒性[J].湖北农业科学,2013, 52(23):5711-5713.
[18] 曹锐. 毒死蜱及TCP对生物DNA损伤和土壤微生物多样性的影响[D].山东泰安:山东农业大学,2009.
[19] 刘祖洞,江绍慧.遗传学实验[M].第二版.北京:高等教育出版社,2002.261-266.
[20] 李宏. 饱和对二氯苯液诱导小麦根尖细胞异常性的研究[J].渝州大学学报(自然科学版),1997,14(1):36-42.
[21] 程文英,杨家宽,丁志圣,等.快中子照射诱发人淋巴细胞微核的剂量效应以及微核和染色体畸变的关系[J].遗传1981,3(3):11-13.
[22] 沈光平,王钦南,周祉祯,等.微核与染色体畸变的相关性[J].遗传,1985,7(1):15-17.
[23] 蒋达和,王桂英,黄佩霞,等.小鼠乳腺腺癌MA782/5s8102系细胞的熟前染色体浓集和微核[J].武汉大学学报(自然科学版),1987(3):102-104.
[24] 屈艾,汪承润,薄军.稀土元素钬对蚕豆的细胞毒性和遗传毒性研究[J].遗传,2004,26(2):195-201.