Effect of heat stress on the gene expression of NMB and its receptor of the hypothalamus-pituitary-testis axis and the secretion of testosterone in rabbit

doi:10.14088/j.cnki.issn0439-8114.2019.12.033

Abstract

Abstract: The aim of this study was to detect the effect of heat stress on the expression levels of Neuromedin B （NMB） and its receptor （NMBR） genes in the hypothalamus-pituitary-testis（HPT） axis of male rabbit, and the secretion of serum cortisol （Cor.） and testosterone （T）. Establishment The heat stress model was established, the expression levels of NMB and NMBR genes in rabbit HPT axis were detected by real-time PCR（RT-PCR）, and the concentration of Cor. and T in rabbit serum were tested using radioimmunoassay （RIA）. Results showed that NMB and NMBR genes were expressed in rabbit HPT axis. The expression pattern of NMB and NMBR mRNA in HPT axis were similar, and heat stress could promote the NMB and NMBR gene expression, which the role was mostly obvious according to the time sequence in the hypothalamus （heat stress 0.5 h）, pituitary （heat stress 1.0 h） and testis （heat stress 2.0 h）; RIA results indicated that heat stress could improve the concentration of Cor. and inhibit T secretion which the inhibition was gradually weakened over time of heat stress. The results indicated that heat stress may regulate the HPT axis through the NMB/NMBR system to affect reproductive function, and then affected the reproduction of animals.

Key words: heat stress, neuromedin B, neuromedin B receptor, hypothalamus-pituitary-testis axis, testosterone

CLC Number:

MA Zhi-yu, ZHANG Ying, CHEN Feng-lei, YANG Sheng, QIAO Wen-na, HU Jia-hui, ZHANG Jin-long, LEI Zhi-hai. Effect of heat stress on the gene expression of NMB and its receptor of the hypothalamus-pituitary-testis axis and the secretion of testosterone in rabbit[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(12): 141-146.

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