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Table of Content

    25 June 2019, Volume 58 Issue 12
    Reviews
    Research advances on protein interaction technology
    LU Yan-yan, WANG Chao, SHI Fu-mei
    2019, 58(12):  5-10.  doi:10.14088/j.cnki.issn0439-8114.2019.12.001
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    Proteins,the true executer of cell activities and functions, play important roles in the proliferation, differentiation, aging and death, and environmental response via complex and highly ordered dynamic interaction. The basic principles and applications of several related research techniques in protein-protein interactions, including yeast two-hybrid system, bimolecular fluorescence complementation assay, phage display technology, fluoresence energy transfer, GST Pull-down, co-immunoprecipitation, and bioinformatics in protein interaction research applications, and their development prospects were also prospected.
    Current situation of breeding and developmental direction about Cucurbita maxima in southern area
    FAN Ai-li, ZHANG Man, HUANG Feng-chan, ZHOU Jian-hui, LIU Wen-jun, CHEN Bao-ling
    2019, 58(12):  11-14.  doi:10.14088/j.cnki.issn0439-8114.2019.12.002
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    Cucurbita maxima is one of the three major cultivation pumpkin. Because of the particularity of the southern area climate, we summarized new varieties, breeding methods and breeding problems of Cucurbita maxima in southern area of China. The combination of traditional breeding technology and modern biotechnology was put forward. The Cucurbita maxima varieties which have health care, high-quality, disease-resistant, storage-resistant characteristics were breeding with early-maturing, cold resistance and mid-late ripening and heat tolerance. The diversified new varieties of edible flowers, leaves and stems in southern China will be the breeding direction of Cucurbita maxima in southern China.
    Research progress and development trends of aquatic products preservation technology
    WANG Hong, WANG Shao-hua, XIONG Guang-quan, BAI Chan, LIAO Tao
    2019, 58(12):  15-18.  doi:10.14088/j.cnki.issn0439-8114.2019.12.003
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    Aquatic products are popular with consumers because of their low fat content, fresh tender in texture and special flavor. However, due to its high water content and high protein content, slaughtered aquatic products is liable to spoilage and their freshness declines rapidly. Therefore, it is urgent to need efficient and safe preservation technology. The research progress of aquatic products preservation at home and abroad in recent years is summarized, the effects of physical, biological and chemical preservation technologies on aquatic products are analyzed, and the development trend of aquatic products preservation in the future is prospected.
    Breeding & Cultivation
    The planting technology of rice Guangliangyou 15 in a rice field with frogs
    JIN Hong-mei, TU Jun-ming, CAO Zhi-gang, CHEN Jie, ZHANG Qun
    2019, 58(12):  19-21.  doi:10.14088/j.cnki.issn0439-8114.2019.12.004
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    The planting technology of rice Guangliangyou 15 in a rice field with frogs was summarized. The sowing time and transplanting time of Guangliangyou 15 were determined by the time when the frog was put into the field and the fertility characteristics of Guangliangyou 15. The sowing amount was determined by the proportion of rice area to frog field, which is half of the sowing amount of conventional rice fields. The spacing of the rows was determined by the habits of the frogs with a larger spacing. Fertilizer application adopts the "One basal application, one topdressing" method, and frog excrement is taken as a supplement. Moisture management adopts aerobic irrigation, which was "shallow-surface-wet" method. The prevention and control of pests and grass pests was based on the principle of prevention and comprehensive prevention and control, and the prevention and control of drugs are not used or less, and the timely harvest is taken. The field fertilization, pest control and control of grass damage measures were best done before the frog delivery.
    Breeding of a new indica hybrid rice variety Yixiangyou 66 with good quality and rice blast resistance
    HUANG Hai-qing, WANG Chang-jiu, LI Hong-sheng, LI Ji-hui, LI Chun-yong, CAI Juan, WANG Gang
    2019, 58(12):  22-23.  doi:10.14088/j.cnki.issn0439-8114.2019.12.005
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    Yixiangyou66 is a new three-line medium hybrid rice variety by Zelong Agricultural Resources Chain(Enshi) Co. Ltd. and Enshi Autonomous Prefecture Academy of Agricultural Sciences, developed by a indica restorer line with rice blast resistance Enhui 66 and Yixiang 1A which breeding by the Academy of Agricultural Sciences of Yibin City, Sichuan Province. Yixiangyou 66 has the characteristics of high-quality and rice blast resistance. It was registered and released for commercial production by Hubei Provincial Crop Variety Appraisal Committee in 2018.
    High yield seed production techniques for blast resistant two-line variety Eliangyou 78
    WU Bo, JIN Wei-bing, XIE Wen-bo, YANG De-li, YIN De-suo
    2019, 58(12):  24-26.  doi:10.14088/j.cnki.issn0439-8114.2019.12.006
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    The new hybrids Eliangyou 78 is crossed by male sterile Enong 1S with the restorer R78 which has good performance in grain quality and blast resistance, but this male sterile has the bad flower habit and smaller angle of glume opening when encountered to high temperature, this made it has difficult in seeds production. According to the characteristics of the outbreeding rate of the female parent to be affected by the high temperature, a set of technical routes suitable for high yield seed production of this combination to avoid heat late sowing were summarized to provide a reference for this combination of seed production.
    Effects of cotton defoliant on seed germination and seedling growth of rapeseed at different sowing dates
    CHENG Hui-huang, CHEN Jun-ying, XIA Shao-nan, SHEN Jia-xing, ZHANG Li-juan
    2019, 58(12):  27-30.  doi:10.14088/j.cnki.issn0439-8114.2019.12.007
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    In order to find out the optimum sowing date of rapeseed after cotton spraying defoliant, Zhongshuang 11 was used as the test material. Five different sowing dates were set up(on October 11, October 14, October 17, October 20, October 23, expressed as T1, T2, T3, T4 and T5). When the sowing date of T2 was completed, defoliant and clear water (CK) were spraying in each plot. The effects of cotton defoliant spraying on seed emergence rate and seedling quality of rapeseed at different sowing dates were studied. The results showed that, the seedling emergence rate and fresh weight of rapeseed seedlings at T1 and T2 were significantly lower than CK after spraying defoliant. Seed germination potential and seedling height of rapeseed at T1 sowing date were also significantly lower than CK. There were no significant differences in seedling emergence rate, germination potential, seedling quality and CK among other sowing dates. Different sowing date had no significant effect on seed germination potential, but had significant effect on seedling traits. The seedling quality between treatments and controls was the best at the seeding date of T3. It can be seen from the above that the optimum sowing time of rapeseed is T3, and spraying defoliant after direct sowing significantly affects the normal germination of rapeseed seeds and seedling growth.
    Effects of planting density on growth and yield of summer direct-seeding cotton
    JI Pan-pan, LI Hong-ju, LUO Dong-yu, HOU Ling, LUO Yan-ping, YANG Fang, WANG Fu, WU Ji-ping, ZHOU Jia-hua
    2019, 58(12):  31-33.  doi:10.14088/j.cnki.issn0439-8114.2019.12.008
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    The effects of five planting densities on main agronomic characters, boll setting and yield of direct-seeded cotton(Gossypium spp) in summer were studied under the condition of one-time flowering fertilization and fixed N, P and K fertilization ratio. The results showed that the boll number and boll weight of direct seeding cotton in summer had obvious effects on yield-related traits when planting density was 67 500~75 000 plants/hm2, and the number of rotten bolls would increase with the increase of planting density. Therefore, the planting density could be used as a reference density for high-yield cultivation of direct seeding cotton in summer.
    Comparative test on special potato varieties of AGRICO series introduced from abroad
    HAN Li-ming, TONG Dan, LIU Da-jiang
    2019, 58(12):  34-38.  doi:10.14088/j.cnki.issn0439-8114.2019.12.009
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    In order to select the high yield, multi-resistance and high quality potato(Solanum tuberosum L.) varieties suitable for Dingxi city planting,in contrast to Favorita, Atlantic and Shepody, a comparative experiment on 10 high quality potato varieties was carried out, through the comprehensive analysis of the characters of various varieties such as high yield,stress resistance,taste and tuber quality,6 special potato varieties with high yield,high quality and drought resistance were screened. Among them, AGRICO-1 and AGRICO-2 meets the requirements for vegetable varieties,comparison with Favorita, the yield increases 16.6% and 32.3%, respectively. AGRICO-5 and AGRICO-6 meet the requirements of fried potato chips,comparison with Atlantic,the yield increases 21.3% and 18.6%, respectively. AGRICAO-8 and AGRICO-9 meet the requirements of fried fries,comparison with Shepody, the yield increases 19.7% and 43.3%, respectively. Comparing with the contrastive group, varieties of AGRICO-2 and AGRICO-9 are particularly outstanding in the margin of yield increase, and they have strong drought resistance and good quality, and have a good prospect for popularization and application.
    Resource & Environment
    Effect of formula fertilization on fertilizer effect parameters of summer peanut
    LIU Xian-yu, SONG Jiang-chun, LI Shuan-zhu, LIU Ting-bao
    2019, 58(12):  39-41.  doi:10.14088/j.cnki.issn0439-8114.2019.12.010
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    The utilization rate, agronomic efficiency and partial factor productivity of fertilizers were studied under conventional and formula fertilization modes to evaluating the application effect of fertilizers in summer peanut. The results showed that compared with conventional fertilization, the fertilizer utilization rate and agronomic efficiency of formula fertilization was higher, and the partial productivity of fertilizer increased by 3.32 kg/kg.
    Effects of nitrogen phosphorus and potassium combination on the yield of potato in Golmud area
    ZHANG Jian-qing, LI Meng, WANG Qin, DING Tao-qun, HU Zhen-ming
    2019, 58(12):  42-44.  doi:10.14088/j.cnki.issn0439-8114.2019.12.011
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    Taking potato variety Xiazhai 65 as the research object, the effects of nutrient absorption, nitrogen, phosphorus, potassium fertilizer utilization, and yield of potato in Golmud area were measured under different treatments. The results showed that the utilization rate of nitrogen fertilizer planted in potatoes was 38.6%, the utilization rate of phosphorus fertilizer was 9.2%, and the utilization rate of potassium fertilizer was 52.3%. In the application of nitrogen 220.00 kg/hm2, the application of phosphorus 158.50 kg/hm2, and the application of potassium 81.30 kg/hm2, the production was relatively high.
    Time series fusion construction of discrete Landsat normalized vegetation index
    ZENG De-yu, LU Xiao-ning, HUANG Yue, YANG Liu-qing, MENG Cheng-zhen
    2019, 58(12):  45-51.  doi:10.14088/j.cnki.issn0439-8114.2019.12.012
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    In combination with the use of sliding windows, a time series construction method based on Fourier approximation and linear fusion downscaling is proposed. The Fourier approximation of discrete Landsat data is performed on a time-by-pixel basis and merged with the lower resolution AVHRR sensor data to realize the time series construction of the Landsat vegetation index in experimental area, combined with Landsat as the actual observation data. The data verifies the accuracy of the model results. The results show thatThe model results in the hue and texture, image features are consistent with the actual observation data, and the NDVI of the white snow-covered area is constructed. The construction results have very good spatial continuity with the surrounding surface, and there is no obvious blocky effect in the entire image; Model results and actual observed data has a high correlation (mean 0.869 2) and a low root mean square error (average of 0.043 5), averaging up to 75.04% of the pixels between ±0.05, up to 97.64% of the pixel error between ±0.1, and the error is in a good normal distribution. The accuracy of the model has a certain dependence on the amount of input data, and there is still room for improvement in the processing of mixed pixels.
    Temporal and spatial dynamic change analysis of vegetation coverage based on RS and GIS in 2006—2011 in Gannan county
    GONG Wen-feng, CAO Kai-hua, ZHOU Ying
    2019, 58(12):  52-57.  doi:10.14088/j.cnki.issn0439-8114.2019.12.013
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    In order to invert the vegetation coverage of the farming-pastoral transition zone by using the Landsat TM/OLI image data of 2006, 2011 and 2016 ,the Gannan county of Heilongjiang province was taken as the study region, based on the RS and GIS technology, the models of normalized difference vegetation index(NDVI) and dimidiate pixel were quantitatively obtained the vegetation coverage of the study area, and the transfer matrix model was used to analyze the temporal and spatial variation characteristics and evolution process of vegetation coverage within 10 years. The results showed that the overall coverage of vegetation cover in the study area was good. The sum of the data coverage and the medium-high coverage area of the three phases in 2006,2011,2016 was 87.29%,87.18% and 87.10%, respectively. In 2006—2011, the vegetation coverage was mainly converted from high coverage and middle coverage to medium and high coverage, and the conversion rates was 76.83% and 55.49%, respectively. In 2011—2016, the conversion between medium and high coverage and high coverage was strong, and the conversion rate was 10.87% and 83.00%, respectively. In the past 10 years, the area ratio of the vegetation change stable area was 64.32% and 65.54%, respectively. 2011—2016 compared with 2006—2011, the area of vegetation degradation decreased by 257 km2, while the area of vegetation improvement increased by 199 km2, and the vegetation was still dominated by degradation.
    Response characteristics of five enzymatic markers in earthworm (Amynthas heterochaetus) under Cd stress
    WU Jing-hui, LI Yong, WEI Yuan, YANG Xiao-jin, ZHANG Yi, HU Qing
    2019, 58(12):  58-63.  doi:10.14088/j.cnki.issn0439-8114.2019.12.014
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    Amynthas heterochaetus, a widely distributed soil species in northern China, was analyzed under heavy metal cadmium (Cd) stress at a semi-lethal dose (LC50). The activities of Amynthas heterochaetus and its five immune-related enzyme markers under Cd stress were analyzed and compared with Eisenia fetida, a standard earthworm species recommended by OECD. The results showed that LC50 of Amynthas heterochaetus(respectively 11.23 and 5.17 μg/cm2) of Cd at 24 and 48 h was lower than Eisenia fetida(respectively 21.99 and 16.95 μg/cm2). Under the low concentration of Cd stress, the activities of SOD, GSH-PX, CAT, AchE and T-AOC were significantly shifted, both in Amynthas heterochaetus and Eisenia fetida. Compared to Eisenia fetida, the response of enzymatic biomarkers in Amynthas heterochaetus were quicker (3 days) and lasted longer (28 days).
    Population structure and spatial distribution characteristics of Populus euphratica in the main stream of Tarim river
    DU Wei-hong, GUAN Wen-ke, HUO Ai-di, LIANG Yan, YI Xiu, WEI Hong
    2019, 58(12):  64-68.  doi:10.14088/j.cnki.issn0439-8114.2019.12.015
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    Using the adjacent grid-like method, 10 plots of 100 m×100 m were set up in the main stream of Tarim river, the quantitative characteristics of Populus euphratica population were analyzed from the aspects of the diameter structure, static life table and distribution pattern of the population, and spatial distribution pattern. The results showed that according to the analysis of the diameter-level structure, the number of young Populus euphratica in the mainstream of the Tarim River was relatively high in the population (Ⅰ~Ⅱ class was 33.47%), belonging to the growth population. In the static life table, the mortality rates of Populus euphratica Ⅰ~Ⅱ class was as high as 39% and 50%, respectively, and the life expectancy of Populus euphratica Ⅲ class was the highest, which was 10.04. The distribution pattern of Populus euphratica population was cluster distribution. The aggregation intensity of Populus euphratica population in the main stream was upstream<midstream<downstream, and the aggregate intensity of Populus euphratica population was about the same in the horizontal stream of 3 000 m.
    Evaluation of soil erosion and its influencing factors in the source of the three rivers region based on continuous replacement method
    YANG Xue-qin, HE Qian, LIU En-qin
    2019, 58(12):  69-73.  doi:10.14088/j.cnki.issn0439-8114.2019.12.016
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    Based on RS and GIS technology, the RUSLE model to evaluate the soil erosion in the source of the three rivers region in 2010 and 2015 was used. At the same time, the continuous substitution method was introduced to explore the influencing factors of soil erosion change,and the multi-factor cross-effect problem was solved. The results showed that the source of the three rivers region presented a large spatial distribution of erosion in the central and eastern parts and less erosion in the east and west. With the increase of elevation and slope length, the soil erosion modulus increased, and the slope length factor increased larger than the elevation. The slope length factor was more sensitive. Overall soil erosion modulus was reduced in 2010—2015, modulus was reduced in the central and western regions, and modulus was increased in the eastern region. The continuous substitution method was better quantitatively described the amount of soil erosion caused by the rainfall and vegetation factors and its spatial distribution. The impact of rainfall on the whole area was large and the degree was low, and the range of vegetation impact was relatively concentrated and deep.
    Forestry volume estimation based on ZY-3 remote sensing image
    ZHANG Xiang-yu, WANG Rui-rui
    2019, 58(12):  74-78.  doi:10.14088/j.cnki.issn0439-8114.2019.12.017
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    Three different methods, principal component regression method, partial least squares method and stepwise regression method are used to establish the volume inversion model based on the satellite image of ZY-3. The stepwise regression model is the optimal model by comparing the accuracy of different estimation models, and the relative accuracy is 95.2%, which meets the needs of forestry investigation.
    Study on ecological manage of urban river channels:A case study of Guizhou Ant River
    LAI Xin, LIU Xiang
    2019, 58(12):  79-83.  doi:10.14088/j.cnki.issn0439-8114.2019.12.018
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    The ecological river management project of Guizhou Ant River from three aspects of dredging, ecotype bank construction and plant configuration on both sides of river bank were analyzes and discusses, and the idea of restoring the river ecosystem and constructing the ecological river were put forward.
    Plant Protection
    The control effect of 330 g/L dimevaline EC,36% isoxazole pine SC on weeds in direct seeding rice field
    MO Ting, SHI Xiao-li, ZHANG Jian-hua, ZHANG Yan, LI Jun, ZHU Long-bao
    2019, 58(12):  84-87.  doi:10.14088/j.cnki.issn0439-8114.2019.12.019
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    The field experiment was conducted to determine the control effect and the optimal dosage of 330 g/L dimevirin EC and 36% isoxazole pine SC on weeds in direct seeding rice fields. The results showed that 330 g/L dimevalin EC, 36% isooxazone SC, or 250 g/L oxazone EC combined with each other had a better control effect on annual weeds in direct seeding rice fields.
    Experiment on chemical control and removal of main weeds in direct seeding rape field in flood and drought rotation area
    LIU Zi-rong, LIU Xiao-juan, XU Xue-liang, HUANG Yan-zhang, WANG Fen-shan, CHEN Qing-long, YAO Ying-juan
    2019, 58(12):  88-91.  doi:10.14088/j.cnki.issn0439-8114.2019.12.020
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    In order to screen the efficient and safety of chemical herbicides applied in direct seeding rape field, as well as in flood and drought rotation field of Jiangxi province, both methods of soil-sprayed pre-emergence and stem and leaf-sprayed post-emergence were applied for testing the control efficacy of several chemical herbicides against the major weeds of Alopecurus aequalis and Beckmannia syzigachne and their bio-safety to the rape. Results indicated that the acetochlor and metolachlor showed good control efficacy after soil-sprayed, the values of control efficacy against the major weeds were 69.2% and 59.0% at 45 days post-treatment, which were significantly superior to the pendimethalin and butachlor, and etc. The sethoxydim and quizalofop-p-ethyl showed good control efficacy and bio-safety after stem and leaf-sprayed post-emergence, the values of control efficacy against the major weeds were 65.9% and 50.0% at 15 days post-treatment, and the weed index were 25.0 and 16.7, respectively. Acetochlor and metolachlor showed a certain side-effect on the emergence of rape because of the high humidity in soil. The oxyfluorfen couldn’t be applied for controlling the weeds of rape field due to its serious phytotoxicity.
    Identification and study of eight endophytic fungi with antimicrobial activity from Lobaria sp.
    FAN Hai-yun, TENG Meng-ting, ZHOU Sheng-liang, MA Lin, LI Ying, LIU Xin-xin, DING Ru-ru, ZHANG Peng
    2019, 58(12):  92-96.  doi:10.14088/j.cnki.issn0439-8114.2019.12.021
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    Using plate separation method, and endophytic fungi was isolated from Lobaria sp. lichens, which were collected from Huanglong and Jiuzhaigou of Sichuan province. These endophytic fungi were inoculated into liquid medium. The antimicrobial activity of the fermentation fluid were respectively evaluated. These fungi were identified by morphology and molecular biology method. The results showed that 37 endophytic fungi were isolated, and 8 of which had antibacterial activity, which belonged to Melanspora, Streptospora, Fusarium and Trichoderma, respectively.
    Horticulture & Local Products
    Study on grafting method of Idesia polycarpa Maxim.
    CHEN Yao-bing, LIU Han-zhen, ZHENG Xiao-jiang, YUAN Bing-wu
    2019, 58(12):  97-98.  doi:10.14088/j.cnki.issn0439-8114.2019.12.022
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    The grafting methods of incision grafting, cleft-grafting, side grafting,inlay grafting and T-bud grafting were carried out to study the effects of different grafting methods on the survival rate and growth potential of Idesia polycarpa Maxim.After grafting for 40 days, the survival strains of Idesia polycarpa Maxim. were observed and recorded under different grafting methods, and 60 days after grafting, the growth status of Idesia polycarpa Maxim. was observed and recorded by different grafting methods. The results showed that the survival rate of the incision grafting was the highest in the branch-grafting, up to 85.0%, and the survival rate of inlay grafting was also high,reached 85.0%. The growth trend of incision grafting is better than that of inlay grafting. The incision grafting in grafting is the best grafting method of Idesia polycarpa. Maxim..
    Resources and industry development status of the Chinese medicinal material in the Hubei Dabie mountain
    WANG Ming-hui, CHEN Zhan-peng, XIONG Fei, HU Hai-zhen, LI Ning, ZHAO Jun-li, HUANG Wei, YIN Hui, SUN Wei, LIU Da-hui, XIE Yan
    2019, 58(12):  99-101.  doi:10.14088/j.cnki.issn0439-8114.2019.12.023
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    The Hubei Dabie Mountain is located in the east of Hubei Province. The resources of the Chinese medicinal materials are abundant. The key regions include Qichun County, Luotian County, Yingshan County and Macheng City, radiating to surrounding areas. The Hubei Dabie Mountain is known as “the resource treasury for medicinal plant of Dabie Mountain”. There are 6×104 hm2 of planting area, 4×108 kg of yield, total output value of 10 billion RMB, more than 30 kinds of genuine medicinal materials, more than 50 kinds of the traditional Chinese medicinal materials, 16 kinds of Chinese medicinal materials products of the geographical indications, 9 kinds of well-known trademarks and 9 kinds of leading enterprises in the Hubei Dabie Mountain. There are many problems about lack of overall planning and scientific layout, hysteretic breeding, less of driving force and deep processing ability, in the Chinese medicinal material industry development. Countermeasures are suggested.
    Effects of different nitrogen application and planting density on the yield and quality of Xianfeng K326 flue cured tobacco
    LI Man-liang, JIANG Sheng-yong, LIU Fan-jun, SONG De-chao, HU Zhou, WANG Bin, YANG Bo, LI Wen-ke, YANG Rui-wei, WEI Guo-sheng
    2019, 58(12):  102-106.  doi:10.14088/j.cnki.issn0439-8114.2019.12.024
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    Taking K326 as experimental material,the effects of different nitrogen application and planting density on the yield and quality of flue cured tobacco were studied. The results showed that, the yield and the production value were highest when the nitrogen application amount was 97.5 kg/hm2. The yield and the production value showed a downward trend with the increase of nitrogen and planting density. At 850 m and 976 m above sea level, the yield and production value increased firstly and then decreased with the increase of planting density,and the yield and the production value were highest when the planting density amount was 15 150 plants/hm2. At an altitude of 1 057 m,the yield and production value showed a tendency to decline firstly and then raised with the increase of planting density. The difference reached a significant level, and the yield and the production value were highest when the planting density amount was 13 890 plants/hm2. With the increase of nitrogen application, the content of nicotine and chlorine increased, and the total sugar decreased. With the increase of planting density, potassium content increased and nicotine content decreased. According to the experimental conclusion, it was suggested that in the tobacco production in Xianfeng County of Hubei province,the nitrogen application amount of 97.5 kg/hm2 was appropriate, and the planting density should be set at 13 890 plants/hm2 between 15 150 plants/hm2.
    Research on application in cigatatte of Coffea arabica L. extracted by different methods
    YANG Shang-ying, LUO Hai-tao, HE Li, LI Hong-xia, LIU Li-fen
    2019, 58(12):  107-111.  doi:10.14088/j.cnki.issn0439-8114.2019.12.025
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    In order to improve the utilization efficiency of Coffea arabica L. in Yunnan, the extracts prepared by heat reflux extraction (HRE) and microwave-assisted extraction(MAE) were analyzed by sensory, volatile components, the application effect in cigarette and phenomenal characteristics of waste coffee grounds. Results showed that MAE was the more efficient extraction method for coffee extract with an improved yield of 13% compared to HRE. Besides, MAE also could enrich fragrance components of extract in order to improve its quality. The extracts of MAE had the more obvious effects than HRE extract on the aspect of improving the sense of sweet, projecting the smell of coffee, balancing the smoke and enriching the aroma of smoking when added to cigarette with 0.1% the weight of cigarette. As a result, MAE is not only a high efficiency method in the extraction of coffee but also a promising application in the field of tobacco flavors.
    Effects of IBA concentration and processing time on hydroponics of Ficus religiosa L.
    WANG Qing, LIU Guo-yu, ZHANG Yan, ZHANG Rui-bo, LI Yan, YANG Qun-li, LIU An-cheng
    2019, 58(12):  112-115.  doi:10.14088/j.cnki.issn0439-8114.2019.12.026
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    In order to improve the cutting propagation efficiency of the Ficus religiosa L., using the half lignified branches of Ficus religiosa L. as the propagating material, rooting effects has been studied after treatment of different IBA concentration and processing time under hydroponic conditions. The results show that the branches can take root after hydroponic 10~20 days under 30 ℃ condition. One leaf treatment can significantly increase the rooting rate, total rooting number and rooting width of Ficus religiosa L. IBA treatment can shorten rooting time and improve rooting rate. The interaction between IBA concentration and treatment time had significant effects on rooting rate and maximum root length, the effect of IBA concentration on the rooting rate and rooting number were significant higher than the treatment time. The optimum concentration of IBA was 100 mg/L, the optimum treatment time was 2 hours.
    Animal Husbandry & Veterinary Medicine
    Isolation and identification of duck capsular serotype A Pasteurella mutocida
    WANG Hong-lin, LU Qin, ZHANG Teng-fei, ZHANG Wen-ting, LUO Qing-ping, WANG Hong-cai, ZHANG Rong-rong, LUO Ling, SHAO Hua-bin
    2019, 58(12):  116-118.  doi:10.14088/j.cnki.issn0439-8114.2019.12.027
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    In order to elucidate the cause of sudden death of laying-duck in a duck farm in Jianli, which the pathogen was determined to be capsular serotype A Pasteurella multocida by bacterial isolation,biochemical identification,mouse pathogenicity test and PCR amplification. The results showed that the isolate had 99.88% homology with Pasteurella multocida, and the PCR product of the capsular antigen serotype-specific gene of the isolate was 99.90% homologous with the Capsular Serotype A, and the pathogen was determined to be Pasteurella multocida strains belonged to capsular serotype A. In order to investigate the drug resistance, the results of drug susceptibility test showed the pathogen was highly sensitive to spectinomycin, moderately sensitive to amikacin, neomycin, norfloxacin, ofloxacin, clindamycin, azithromycin, while resistance to ceftriaxone, streptomycin, co-trimoxazole, sulfisoxazole, fluoride benignic, tetracycline, nitrofurantoin, lincomycin, which provided guidance for clinical rational medication.
    Preliminary report on selecting experiment of silage maize
    FU Zhong-jun, ZHAO Li, LI Shu-jun, YANG Hua, WU Yuan-qi, QI Zhi-yun
    2019, 58(12):  119-123.  doi:10.14088/j.cnki.issn0439-8114.2019.12.028
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    Using 34 cultivars in southwest China as experimental materials, through 9 experiment places in different ecological areas in southwest China, the characteristics, silage yield and quality of different cultivars were analyzed under planting density of 5 000 plants/mu. Five cultivars with yields exceeding 8% of the control Yayu Silage 8 were selected, which were Yuqing 506, Yuqing 386, Yayu Silage 988, Dika 008 and Beinong Silage 368. These varieties not only have high yield and good quality, but also have good disease resistance, lodging resistance and comprehensive properties. They are suitable for planting as silage maize in southwest China.
    Effect of fermentation level of dark brick tea on improving gastrointestinal function in mice
    TANG Fei, ZHANG Yue-feng, QU Feng-feng, CHEN Yu-qiong, YU Zhi, NI De-jiang
    2019, 58(12):  124-129.  doi:10.14088/j.cnki.issn0439-8114.2019.12.029
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    The aim of this study was to examine whether different fermentation levels of dark brick tea affected the gastric emptying, intestinal propulsion and intestinal microflora in Kunming mice by conducting correlation analysis between major chemical components of dark brick tea and gastrointestinal function of mice. Results indicated that during the fermentation process, the contents of free amino acids, polyphenols, soluble sugars, water extracts, theaflavins, thearubigins, epigallocatechin, epicatechin, catechin, epigallocatechin gallate, tartaric acid, malic acid and lactic acid were significantly decreased, while the contents of theabrowine, caffeine, oxalic acid, quininic acid, succinic acid as well as gallic acid were remarkably increased. After a consecutively 15 days intragastric administration of dark brick tea extract solution to mice, both dark brick tea fermented for 9 days and 15 days could significantly promote gastric emptying and intestinal propulsion, increase the amount of bifidobacterial and lactobacillus, as well as decrease the amount of enterobacteria and enterococcus, in which of the dark brick tea fermented for 15 days was better. Furthermore, the dark brick tea fermented for 15 days was superior to 9 days in terms of improving gastrointestinal function of mice. Correlation analysis showed the major functional components of dark brick tea might be theabrowine, oxalic acid, quininic acid, succinic acid, citric acid and gallic acid.
    Storage & Processing
    Application of antibacterial activity of chitosan in pickles
    DONG Hong-bing, ZHAN Xiao-yun
    2019, 58(12):  130-133.  doi:10.14088/j.cnki.issn0439-8114.2019.12.030
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    Through the sensory evaluation, moisture content, pH value, the natural preservative antistaling agent present situation and research progress of chitosan, and the application of natural preservatives chitosan in pickles was carried out. The results showed that the concentration of 1.0% of antibacterial activity of chitosan solution, the strongest in the normal temperature storage of pickles has the best effect of antisepsis, at the same time, chitosan has a certain effect to prevent moisture loss;Concentration bacteriostatic effect of 2.0% chitosan solution and sodium benzoate are similar, but the role of chitosan to the pH value is not obvious, is better than sodium benzoate.
    Comparison of two methods for the determination of phytase activity and fermentation conditions
    XU Dan-dan, ZHAO Han-dan, WANG Xiu-ran, GUAN Shu-yan
    2019, 58(12):  134-137.  doi:10.14088/j.cnki.issn0439-8114.2019.12.031
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    The soil sample was collected from the organic-rich environment, and phytase production strains were obtained by preliminary screening with calcium phytate plate transparent ring method. Then phytase activity was determined by two different methods vanadium-ammonium molybdate method and acetone-ammonium phosphomolybdate method, and then compared. The results showed that the method of vanadium-ammonium molybdate was accurate and available. The orthogonal fermentation test determines that pH is 5, the yield of soluble enzyme is 1.25 g/100 mL, the optimum dosage of yeast powder is 1.80 g/100 mL, and the amount of calcium phytate is 1.0 g/100 mL.
    Research on antioxidant and antitussive and expectorant activity of different components from loquat leaves
    HUANG Su-hua, CHEN Tong, QIU Feng-yan, HONG Yan-ping
    2019, 58(12):  138-140.  doi:10.14088/j.cnki.issn0439-8114.2019.12.032
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    Determination of flavonoid, polyphenol, total terpene and their contents in loquat[Eriobotrya japonica (Thunb.) Lindl.] leaf extracts by spectrophotometry, DPPH and FRAP method were used to determine the antioxidant activity, cough model induced by ammonia liquor in mice to observe the antitussive effects,the method of phenol red execretion in mice was used to investigate expectorant effects,and the correlation of composition and the activity were analyzed. Results showed that the loquat leaf extracts had certain antioxidant activity, the flavonoids were significant better than triterpenoid acid, the polysaccharides were weaker, and the antioxidant activity related to the content of flavonoids and total polyphenols. All the 6 kinds of loquat leaf extract had certain antitussis and expectorant activity, the flavonoids and triterpenoid acid were better than the polysaccharides, the times of cough and the content of flavonoid showed significantly negative correlation, the saponins had certain dosage-effect relation with prolonged the incubation period of cough and expectorable activity. The extracts of loquat leaf had antioxidation and antitussis and expectorant activity, flavonoids and polyphenols could be important part of the antioxidant component, flavonoids and saponins were associated with antitussis and expectorant activity. Through the refined, the effective components can be enriched and the relevant activity can be improved.
    Biological Engineering
    Effect of heat stress on the gene expression of NMB and its receptor of the hypothalamus-pituitary-testis axis and the secretion of testosterone in rabbit
    MA Zhi-yu, ZHANG Ying, CHEN Feng-lei, YANG Sheng, QIAO Wen-na, HU Jia-hui, ZHANG Jin-long, LEI Zhi-hai
    2019, 58(12):  141-146.  doi:10.14088/j.cnki.issn0439-8114.2019.12.033
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    The aim of this study was to detect the effect of heat stress on the expression levels of Neuromedin B (NMB) and its receptor (NMBR) genes in the hypothalamus-pituitary-testis(HPT) axis of male rabbit, and the secretion of serum cortisol (Cor.) and testosterone (T). Establishment The heat stress model was established, the expression levels of NMB and NMBR genes in rabbit HPT axis were detected by real-time PCR(RT-PCR), and the concentration of Cor. and T in rabbit serum were tested using radioimmunoassay (RIA). Results showed that NMB and NMBR genes were expressed in rabbit HPT axis. The expression pattern of NMB and NMBR mRNA in HPT axis were similar, and heat stress could promote the NMB and NMBR gene expression, which the role was mostly obvious according to the time sequence in the hypothalamus (heat stress 0.5 h), pituitary (heat stress 1.0 h) and testis (heat stress 2.0 h); RIA results indicated that heat stress could improve the concentration of Cor. and inhibit T secretion which the inhibition was gradually weakened over time of heat stress. The results indicated that heat stress may regulate the HPT axis through the NMB/NMBR system to affect reproductive function, and then affected the reproduction of animals.
    Expression of related genes of VB6 metabolic pathway in fruits of apple bitter disease
    LIU Lian-mei, QU Hai-youg, AN Shuang, HAO Chuan-hao, REN Cui-ping
    2019, 58(12):  147-151.  doi:10.14088/j.cnki.issn0439-8114.2019.12.034
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    In this study, the diseased fruit and non-pathogenic fruit of Fuji apple with a storage period of 0 days were used as control materials. The expression of genes related to VB6 metabolic pathway in normal fruit (CK), diseased fruit non-diseased parts (BC) and diseased fruit diseased parts (BP) was studied by High-throughput sequencing, extraction of pulp RNA, cdna reverse transcription and fluorescence quantitative PCR techniques, and the differences among them were compared. After experiments, the results showed that the relative expression levels of genes related to VB6 metabolic pathway in CK, BC and BP were different, and they were significantly upregulated in BP. Thus, the VB6 metabolic pathway is associated with apple bitter pit disease and gene expression is upregulated. Therefore, for the follow-up study of apple and other diseases such as bitter pit disease, we can start from the breakthrough of the VB6 metabolic pathway molecule.
    Economy & Management
    Study on the intellectual protection property and the inheritance and development of agricultural culture of Chinese medicine Coptis
    SUN Yuan-peng, LIU Yu-si, CHENG Zheng, WU Zhe, SUN Yan-ling
    2019, 58(12):  152-155.  doi:10.14088/j.cnki.issn0439-8114.2019.12.035
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    Starting from Coptis and its medicinal value, the traditional knowledge, agricultural biological genetic resources, geographical indications, new plant varieties, and agricultural intellectual property resources such as agricultural patents and trademarks are studied. The main problems of Coptis agricultural intellectual property protection and inheritance and development of agricultural culture are analyed, and the countermeasures and suggestions for the improvement of Coptis agricultural intellectual property protection and agricultural culture inheritance and development under the background of rural revitalization strategy and innovation-driven development strategy and high-quality agricultural development are put forward.
    Social network analysis of rural kindergartens collaborative management:Taking Urumqi city as an example
    JIANG Nan, ZHANG Ye
    2019, 58(12):  156-160.  doi:10.14088/j.cnki.issn0439-8114.2019.12.036
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    The social network analysis method is used to study the synergy relationship between rural kindergarten management departments in Urumqi. Based on 177 participants' interaction events, UCINET software is used to systematically analyze the network density, centrality, and condensed subgroups of rural kindergarten organization management in Urumqi. On the basis of this, QAP technology is used to reveal the important factors affecting the construction of rural kindergartens' management network in Urumqi City. It is proposed that the development of rural kindergartens needs to be coordinated by the county education bureau, the collaborative management of various government departments, and the active participation of social forces in the organization and management network. At the same time, it is necessary to build a collaborative platform to provide an effective information communication platform for diverse managers. At the same time, it is necessary to build a collaborative platform to provide an effective information communication platform for diverse managers.
    Analysis on the job satisfaction of village cadres and its influencing factors under the background of rural revitalization:Based on the survey of 210 village officials in Zhejiang province
    YANG E, QI Di-ming
    2019, 58(12):  161-166.  doi:10.14088/j.cnki.issn0439-8114.2019.12.037
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    Based on the survey data of 210 village cadres in Wenzhou, Huzhou and Quzhou in Zhejiang province, using factor analysis, multiple regression and quantile regression, the influencing factors of village cadres' job satisfaction are empirically tested. The results show that the education level, income and current development evaluation of village cadres have a significant positive impact on their job satisfaction, that is, the higher the education level of village cadres, income and evaluation of the village development, the higher their job satisfaction. It is proposed that the construction of a comprehensive salary incentive, political incentives and reputation incentive mechanism should improve the job satisfaction of village cadres and stimulate their internal motivation to better promote rural revitalization.
    Research on cultural construction of leading enterprises in agricultural industrialization:Taking the image sculpture of Wuliangye group Co.,Ltd. as an example
    YANG Yu-quan
    2019, 58(12):  167-169.  doi:10.14088/j.cnki.issn0439-8114.2019.12.038
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    Through the aspects of modeling, material, size, color, lighting, pedestal, etc., the iconic image of Wuliangye Group, the leading enterprise of agricultural industrialization, Ende Tower, is deeply studied to analyze the positive impact of cultural construction on improving corporate culture and art image, beautifying the environment, and promoting economic benefits. It points out the importance of strengthening the cultural construction of leading enterprises in agricultural industrialization.
    Research on the role of financial innovation in rural economic development
    ZHANG Chun-yu
    2019, 58(12):  170-174.  doi:10.14088/j.cnki.issn0439-8114.2019.12.039
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    Financial innovation can promote economic development from the aspects of transforming economic structure and reducing transaction and risk management expenditure. Using the panel data of China's rural areas from 2008 to 2017, the rural loan conversion rate, rural financial structure, rural financial efficiency and the average GDP of the rural primary industry laboratories were used as indicators to verify the role of rural financial innovation in promoting rural economic development. Based on the predicaments of financial innovations in the current rural economic development, such as systems,markets, services and products, the countermeasures and suggestions for clearing the path of institutional reform, innovating market and service system and credit products are proposed.
    The impact of employment structure on land development degree in China based on geographical detector
    LI Ya-ya
    2019, 58(12):  175-179.  doi:10.14088/j.cnki.issn0439-8114.2019.12.040
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    Using geographic detector to reveal the impact of employment structure on land development degree (LDD) and its regional differences,which provides mechanistic information for formulating different employment support policy and rational control of construction land scale. The results showed that, during 2000—2016, the number and proportion of the first-class are declining continuously, the number and proportion of the second-class employees are increasing at first and then slowly declining, and the number and proportion of the third-class employees is increasing steadily, and it shows a significant growth after 2012. There are differences in the changes of employment structure among the three regions of the east, the middle and the west. During 2009—2015, employees in the first, second and third industries all have a significant impact on LDD in China. The dominant driving factors of LDD in the three regions are different. The employment population of the second and third industries in the eastern region has a significant impact on LDD, and the second industry practitioners in the central and western regions have a significant effect on LDD. There are also regional differences in the impact of different employment sectors on LDD.
    Rural Revitalization
    The enlightenment of Taiwan rural community construction to the transformation and upgrading of Huzhou rural tourism
    ZHU Zhi
    2019, 58(12):  180-183.  doi:10.14088/j.cnki.issn0439-8114.2019.12.041
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    After more than 60 years of development, the Taiwan community has accumulated rich experience in building rural communities. Through the research on the construction mode of rural communities in Taiwan, it is found that there are four main types, characteristic industry type, local ecological education type, national culture type and cultural creativity type. The tourism development and construction of these rural communities are of great significance to the transformation and upgrading of rural tourism in Huzhou.
    Research on the influence of social network heterogeneity on the preference of rural households' online shopping model
    LI Yan-jun, WANG Dan, ZOU Xiao-hong
    2019, 58(12):  184-188.  doi:10.14088/j.cnki.issn0439-8114.2019.12.042
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    According to the social network theory and trust theory, the mechanism model of the influence of farmers' social network heterogeneity on the preference of agricultural net purchase model is constructed, and an empirical test is conducted through 345 household survey questionnaires. The results show that the heterogeneity of farmers' social networks has a significant impact on their agricultural net purchase model preferences and transaction trust. The higher the trust of farmers with high online shopping heterogeneity, the more they prefer to choose the independent online shopping model; the farmers with low network heterogeneity trust the agricultural retailers and prefer the offline service station purchasing model; brand trust and retailer trust play a mediating role in the heterogeneity of farmer networks and their preferences for online shopping.
    Study on people's cognition and response to landslide and debris flow disaster in Qinba mountain area:A case study of Shangnan county,Shaanxi province
    TIAN Meng-yu, SONG Dian-xing
    2019, 58(12):  189-192.  doi:10.14088/j.cnki.issn0439-8114.2019.12.043
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    In order to reduce the damage of landslide and debris flow disaster to the ecological environment of the region and promote the ability of regional sustainable development,the method of questionnaire survey was adopted, based on the evaluation model of cognition and response of landslide and debris flow disaster, the cognition and response of landslide and debris flow disaster of people were studied in Shangnan, Shaanxi province. The results showed that we could enhance the comprehensive ability of cognition and response to landslide and debris flow disasters in the region by using schools and grass-roots organizations,and strengthen the training of disaster prevention and mitigation skills,which was one of the important ways to improve the comprehensive ability of cognition and response of regional landslide and debris flow disasters.