Abstract: To explore the antibacterial activity of wood vinegar, the wood vinegar of serayah was used as raw materials to measure the basic physical and chemical properties. The crude wood vinegar was refined by static adsorption combined with activated carbon adsorption. The difference between the crude wood vinegar and the purified wood vinegar was compared by gas chromatography-mass spectrometer（GC-MS） and the purified wood vinegar were disposed of dilution, neutralization, distillation and extraction, respectively. It was determined on effective antibacterial time and the best observation time of the wood vinegar through experiments. The purified and disposed wood vinegar was used for bacteriostatic experiments. The bacteriostatic effects of wood vinegar were studied on mildew in different treatment conditions which were purification, pH regulating, dilution, decompression distillation and extraction, respectively. The results showed that the main components were acids, phenols, ketones and alcohols in wood vinegar and the effective antibacterial time was 48 h for mildew. The minimum bacteriostatic concentration was V_{refined wood vinegar}∶V_{sterilized water}=1∶20, and the optimum pH was 4. The appropriate temperature of decompression distillation was 60 ℃ and the best extraction solvent was ethyl acetate.

Key words: serayah, wood vinegar, component analysis, antibacterial activity