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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails Select Effect of medium cost reduction measures on the growth of sweet potato tissue culture seedlings under continuous subculture ZHAO Yuan-zeng, WANG Yong-gang, CHEN Dan HUBEI AGRICULTURAL SCIENCES    2024, 63 (4): 179-184.   DOI: 10.14088/j.cnki.issn0439-8114.2024.04.033 Abstract （26）      PDF （12826KB）（5）       Knowledge map    Save In order to reduce the production cost of rapid propagation of sweet potato （Dioscorea esculenta （Lour.） Burgill） tissue culture seedlings and improve the economic benefits of factory seedling cultivation,on the basis of MS basic culture medium, the effects of simplifying or replacing the composition of culture medium on the growth of sweet potato tissue culture seedlings under continuous subculture were studied through cost reduction measures such as simplifying the composition of culture medium, replacing deionized water with tap water, and replacing sucrose with granulated sugar. The results showed that using white sugar instead of sucrose had no significant effect on the growth of sweet potato tissue cultured seedlings, whether in primary or secondary culture. However, removing trace elements and organic matter from the culture medium, or using tap water instead of deionized water, all had adverse effects on tissue culture seedlings, leading to a decrease in plant height, a decrease in proliferation coefficient, and poorer root growth,and this adverse effect was further exacerbated after prolonged subculture, resulting in poorer growth of tissue cultured seedlings. Therefore, in the factory production of sweet potato tissue culture seedlings, white sugar could be used instead of sucrose to reduce the production cost of tissue culture, while other cost reduction measures were not suitable. Reference | Related Articles | Metrics Select SNP analysis of the ovarian transcriptome of Columba livia with different egg production numbers MAO Nan-nan, SUN Yong-sheng, CHEN Hui, ZHOU Rong-yan, JI Ying HUBEI AGRICULTURAL SCIENCES    2024, 63 (4): 185-190.   DOI: 10.14088/j.cnki.issn0439-8114.2024.04.034 Abstract （29）      PDF （3172KB）（4）       Knowledge map    Save In order to explore the genes and genetic markers related to egg production in Columba livia, STAR was used to compare high-quality transcriptome sequences of ovarian tissue transcriptome data from high and low egg production Columba livia with the reference genome,after removing duplicate sequences, single nucleotide polymorphism （SNP） screening was performed using GATK. After controlling SNP quality using VCFtools, principal component analysis, evolutionary tree construction, and Fst calculation were performed. KEGG enrichment analysis was used to identify the gene where the SNP was located. The results showed that the high-yielding egg group had a total of 298 957 SNPs, the low yielding egg group had a total of 296 137 SNPs, and the high-yielding and low-yielding egg groups had a total of 254 118 SNPs. The KEGG analysis results showed that the SNP gene was significantly enriched in the progesterone regulated signaling pathways of oocyte maturation, meiosis, and metabolism;102 specific SNPs were distributed on 14 annotation genes（LOC110357454, KDM5A, PIGS, BNC2, GBF1, SLAIN2, FGD4, DIAPH2, KIF3B, NFIB, EPG5, OSTF1, EMB, and ST3GAL3） in the high and low egg production groups, and the enriched signaling pathways may be closely related to their egg production ability. Reference | Related Articles | Metrics page Page 1 of 86 Total 172 records First page Prev page Next page Last page