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Genetic structure analysis of 121 wild goji berry germplasms based on SSR fluorescence markers
DAI Guo-li, LIU Na, QIN Ken, ZHANG Bo, YIN Yue, MI Ja, HE Xin-ru
HUBEI AGRICULTURAL SCIENCES
2024, 63 (5):
207-214.
DOI: 10.14088/j.cnki.issn0439-8114.2024.05.036
121 wild goji berry（Lycium barbarum L.） germplasms were collected as materials, 9 pairs of SSR primers were used of amplification, and polymorphism was analyzed using GeneALEX and Power Marker software. Genetic diversity analysis, cluster analysis, and principal component analysis were performed using NTSYS and STRUC-TURE software.The results showed that a total of 108 alleles were detected using 9 pairs of SSR primers. The number of alleles （Na）, effective alleles （Ne）, observed heterozygosity （Ho）, expected heterozygosity （He）, polymorphic information content （PIC）, and Shannon’s information index were 12, 4.620, 0.678, 0.651, 0.629, and 1.568, respectively;the clustering results showed that 121 wild goji berry germplasms could be divided into 5 subgroups. The genetic structure of the population showed that when K=5, 121 germplasms could be divided into 5 subgroups. The genetic distance between NXGY-02 and NXGY-03 germplasms from Ningxia and 6 germplasms from Inner Mongolia was the farthest, and the genetic relationship was relatively distant,there was extensive gene exchange among other wild goji berry germplasms from Qinghai Province, Xinjiang, Gansu Province, and Inner Mongolia;the wild goji berry germplasms in Ningxia and Inner Mongolia had the possibility of independent origin and evolution, while the germplasms in Xinjiang, Gansu, and Qinghai provinces were closely related to the germplasms in Ningxia and were likely to belong to the same origin.
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