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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails Select Community structure and spatial distribution characteristics of culturable bacteria in Dongting lake SUN Xia, LIU Yang, WANG Fang, ZHANG Hu, MAO Shu-min, QI Cui-ying HUBEI AGRICULTURAL SCIENCES    2022, 61 (7): 13-19.   DOI: 10.14088/j.cnki.issn0439-8114.2022.07.003 Abstract （374）      PDF （3194KB）（187）       Knowledge map    Save The physical and chemical characteristics of water and sediment samples in Dongting lake area were analyzed, and the bacterial community structure was investigated by culture-dependent method and 16S rRNA gene sequence analysis. The results show that the water body in Dongting lake is neutral to slightly alkaline, and the comprehensive trophic level index （TLI） of East, South and West Dongting lake are 43.77,46.26 and 48.16, respectively, all of which belong to mesotrophic state. A total of 94 bacterial strains are obtained from water and sediment samples of Dongting lake, belonging to 4 phyla,17 genera and 41 species. The dominant phylum is Proteobacteria, followed by Firmicutes, and the dominant genus is Acinetobacter. The bacterial community structure is quite different in spatial distribution. Among 17 genera obtained from water and sediment samples, only five genera are shared, eight genera are unique to water samples, and four genera are unique to sediment samples. In addition, the species richness and abundance of bacteria in water and sediment samples of East Dongting lake are higher than those in the other two lakes. The results further reveal that Dongting lake area is rich in bacteria resources, and the differences in spatial distribution of bacterial communities are comparatively obvious. The diversity of bacterial communities decreased with the increase of eutrophication level. All of the above indicates that the lake area has been polluted at different levels, which leads to the differences in physical and chemical indicators of the water quality, and then affects the structure and diversity of bacterial community. Therefore, changes in physical and chemical factors caused by the external pollution have a greater impact on the microbial community structure and diversity. Reference | Related Articles | Metrics Select Research on preparation of seaweed organic liquid fertilizer by composite enzmy coupled microbial fermentation QI Shou-he, LI Hao-yuan, ZHANG Heng, KONG Fan-ke, QU Wei HUBEI AGRICULTURAL SCIENCES    2022, 61 (7): 20-24.   DOI: 10.14088/j.cnki.issn0439-8114.2022.07.004 Abstract （372）      PDF （1605KB）（294）       Knowledge map    Save In order to develop marine biomass resources, seaweed organic liquid fertilizer was prepared by biological method with macroalgae as raw material. Compound enzymolysis coupled microbial fermentation method was used. The factors affecting enzymatic hydrolysis efficiency, such as pH, temperature, enzyme dosage and reaction time, were studied. The optimum technological parameters were obtained by orthogonal experiment. In order to further improve the release of bioactive substances in seaweed cells, the optimal fermentation conditions were obtained by further fermentation of seaweed hydrolysate with microbial agents. The results showed that the optimal enzymolysis conditions for the preparation of seaweed fertilizer by compound enzymolysis coupled with microbial fermentation were as follows： pH 6.5, enzymolysis temperature 65 ℃, enzyme dosage 5%, enzymolysis time 8 h. The optimal fermentation conditions were as follows： fermentation time 36 h, inoculation amount of microbial agent 0.3%, bottling amount 40 mL/100 mL, fermentation temperature 45 ℃, initial fermentation pH 6.5. Through seed germination test, seaweed liquid fertilizer prepared by compound enzyme hydrolysis coupled with microbial fermentation can significantly improve seed germination rate. Reference | Related Articles | Metrics page Page 1 of 5 Total 9 records First page Prev page Next page Last page