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    Comparison of anesthetic effect of MS-222 and eugenol on Xenocypris davidi
    CHEN Jun-ping, WU Hui-hui, SHEN Fang-fang, ZHANG Jia-xin, YU Ruo-meng, FU Yong-jie, ZHAO Dao-quan, XIE Guo-qiang, SUN Wen-xi
    HUBEI AGRICULTURAL SCIENCES    2023, 62 (8): 133-139.   DOI: 10.14088/j.cnki.issn0439-8114.2023.08.021
    Abstract257)      PDF (1773KB)(173)       Save
    In order to study the anesthesia effect of MS-222 and eugenol on Xenocypris davidi, the Xenocypris davidi with a body weight of (20.10±1.28) g was selected and the anesthesia effect of the Xenocypris davidi was observed under different conditions. The results showed that with the increase of the concentrations of the two anesthetics, the time spent in anesthesia for Xenocypris davidi showed a decreasing trend, the recovery time of the MS-222 group was shortened, and the recovery time of eugenol was prolonged. When the mass concentration of MS-222 was 120 mg/L, or the mass concentration of eugenol was 30~60 mg/L, the Xenocypris davidi could be anesthetized within 3 minutes and resuscitated within 5 minutes. With the increase of the concentration of the two anesthetics, the respiratory rate of the experimental fish first increased and then decreased. Under the condition of the same mass concentration of the two anesthetics, with the continuous increase of the water temperature, the anesthesia time of the Xenocypris davidi showed a decreasing trend. And the recovery time of the eugenol treatment group continued to shorten, while the recovery time after MS-222 anesthesia was not significantly different at 10~20 ℃, and was significantly prolonged at 25 ℃(P<0.05). After anesthesia, with the increase of exposure time in the air, the resuscitation time of the MS-222 group showed a continuously prolonged trend, and the resuscitation time of the eugenol group was shortened at 4 minutes, and then continued to prolong. The MS-222 of 120 mg/L and eugenol of 30~60 mg/L had good anesthesia effect on Xenocypris davidi, and both could be used as ideal anesthetics for Xenocypris davidi.
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    Effects of environment on the artificial breeding of Charybdis feriatus and the growth of juvenile crab
    ZOU Xiong, YANG Ming-qiu, PU Li-yun, LU Jian-xue, XIA Lian-jun, LIU Xin
    HUBEI AGRICULTURAL SCIENCES    2023, 62 (8): 140-143.   DOI: 10.14088/j.cnki.issn0439-8114.2023.08.022
    Abstract375)      PDF (3315KB)(108)       Save
    The impact of the current aquaculture environment on the artificial reproduction and juvenile crab growth of Charybdis feriatus was studied, and the breeding density test, bait test and shelter test were conducted. The results showed that, ①the weight of Charybdis feriatus juvenile crab in each stage increased first and then decreased with the increase of breeding density. In the second stage, the weight of D2 group juvenile crab was the largest, indicating that the breeding density of 150 per square meter could ensure the normal development of juvenile crab weight; ②The biological feed feeding group had the lowest body weight of Charybdis feriatus juvenile crab, while the mixed feed feeding group had the highest body weight of Charybdis feriatus juvenile crab. The biological feed feeding group had the lowest shell width and length, and the survival rate of Charybdis feriatus juvenile crab was inversely proportional to the incubation period of the juvenile crab; ③There was a significant difference in the body weight of Charybdis feriatus juvenile crab between different shading test groups. The S1 and S3 groups of Charybdis feriatus juvenile crab had higher body weight values. Under the condition of no shelter, the body weight value of the Charybdis feriatus was the lowest.
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