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Published in last 1 year |  In last 2 years |  In last 3 years |  All Please wait a minute... For Selected: Download Citations EndNote Reference Manager ProCite BibTeX RefWorks Toggle Thumbnails Select Artificial breeding technology of Charybdis feriatus ZOU Xiong, YANG Ming-qiu, PU Li-yun, LU Jian-xue, XIA Lian-jun, LIU Xin HUBEI AGRICULTURAL SCIENCES    2023, 62 (9): 97-100.   DOI: 10.14088/j.cnki.issn0439-8114.2023.09.018 Abstract （143）      PDF （1845KB）（43）       Knowledge map    Save Taking Charybdis ferriatus as the research object, the developmental stages of Charybdis ferriatus were divided into 7 stages： T1, T2, T3, T4, T5, T6, and M stages for artificial seedling cultivation experiments. The results showed that ① from the average stage survival rate of crab seedlings, the highest survival rate in autumn and spring was in the T1 stage, which was 100%; The lowest survival rate was at the stage of juvenile development, which was 42.5%; the second lowest survial rate was at the large eye larva stage, accounting for 51.1%. During the breeding process of Charybdis pumila, the T6 stage metamorphosis to the large eye larva and the large eye larva metamorphocis to the I stage juvenile crab were the most important stages. ②The overall survival rate of the crab seedlings from the rust spotted crab to the T3 stage in spring and autumn was 48.1%, the overall survival rate of the T6 stage metamorphosis to the large eye larvae stage was 11.4%, and the overall survival rate of the seedlings that eventually developed into the I stage juvenile crab was 5.0%. Reference | Related Articles | Metrics Select Study on anti-thrombotic effect of Ampelopsis grossedentata composite juice in rats XIE Yong, FAN Zhi-ping, SHI Jia, LIU Qing-qing, GAO Jian-qiang HUBEI AGRICULTURAL SCIENCES    2023, 62 (9): 101-107.   DOI: 10.14088/j.cnki.issn0439-8114.2023.09.019 Abstract （146）      PDF （3392KB）（30）       Knowledge map    Save The Ampelopsis grossedentata （AG） composite juice was prepared, followed by investigating its anti-thrombotic effect in rats. The AG composite juice was used to constantly intervene in the thrombus rats model, that was successfully established by using ligation of the inferior vena cava. The DHM solution and AG juice were used as controls. Rats were then sacrificed prior to determining the weight, length of the thrombus samples, and the antithrombotic rate. Consequently, the physicochemical properties of thrombus samples were measured by using HE staining and immunohistochemistry. Results showed that the content of total flavonoids （1.43±0.11 mg/mL） and DHM （0.66±0.05 mg/mL） in composite juice were significantly lower than those in control groups. Moreover, the length （10.98±1.20 mm）, wet weight （70.6±15.4 mg） and dry weight （49.9±3.2 mg） of thrombus in the model group were obviously higher than those in the normal group. Compared with other invention groups, the length of thrombus （3.92±0.64 mm） and the ratio of thrombus area to the entire inner surface area of blood vessels （32.1±1.4）% decreased significantly in the AG composite juice group, while the thrombus inhibition rate （64.5±1.3）% and dissolution rate （54.2±1.3）% increased significantly. Additionally, the HE staining and immunohistochemistry indicated that the AG composite juice group exhibited lower degree of vascular cell damage in integrity and nuclear fixation, relative expression level （25.2±2.1）% of TBXAS1, as well as a higher positive cell rate compared to other intervention groups. The AG composite juice had a favorable anti-thrombotic effect. Reference | Related Articles | Metrics