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    Effect of spraying lime sulfur on promoting fruit coloring of orah
    HUANG Qi-chun, TAN Song-yue, WANG Ni-na, LIU Fu-ping, LIU Ji-min, HU Cheng-xiao, LIAO Hui-hong, HUANG Hong-ming, WEI Chi-zhang, CHEN Dong-kui, ZENG Zhi-kang
    HUBEI AGRICULTURAL SCIENCES    2024, 63 (4): 73-77.   DOI: 10.14088/j.cnki.issn0439-8114.2024.04.014
    Abstract30)      PDF (2589KB)(6)       Save
    In order to verify the effect of spraying lime sulfur on the fruit coloring of orah, different treatments of lime sulfur were carried out in Chengxiang Town, Wuming District, Nanning City, Guangxi Province. The results showed that in the key period of maturity and color transformation(December 30th), the spray of lime sulfur had a significant effect on promoting the red transformation and coloring of orah. A single spray of lime sulfur 1.2 Baume degree had the most significant effect on promoting the red transformation and coloring, and the redness value a* increased by 15.24% compared with CK. The overall coloration index CCI of orah was increased by 40.07% compared with CK, both of which reached the level of extremely significant differences. After a single spraying of 0.8 Baume degree, a* and CCI increased by 11.67% and 32.31% respectively compared with CK. All treatments sprayed with lime sulfur showed an improvement effect on reddening and coloring. According to field observation, the lime sulfur also had good control effect on diseases and pests such as canker, gray mold, aphids, thrips, scale insects, red spiders, etc.
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    Determination of pollen ploidy of cabbage by flow cytometry
    YANG Ding, LI Chong-juan, LYU Feng-xian, HE Jiang-ming, LAN Mei, HU Jing-feng, XU Xue-zhong
    HUBEI AGRICULTURAL SCIENCES    2024, 63 (4): 78-81.   DOI: 10.14088/j.cnki.issn0439-8114.2024.04.015
    Abstract31)      PDF (3102KB)(4)       Save
    Pollen ploidy of Brassica oleracea var. capitata was determined by flow cytometry. Pollen of cabbage GL21028 was used as the material, and leaves were used as the control to compare the effects of two pollen treatments, three different cell lysates, and two nucleic acid dyes. Pollen cells obtained through B buffer could be directly stained, which was the easiest treatment method. Cabbage leaves and pollen were treated with “Aru” buffer and Y lysates and stains DAPI, and flow cytometry was used for detection. The DNA of the sample cells was concentrated, and there were few cell fragments. The prepared Y lysate could be used for the observation of cabbage material by flow cytometry. Cabbage pollen treated with B buffer could be directly used as the material for flow cytometry ploidy identification, no other treatment was required, and the prepared Y lysate could obtain better test results.
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