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Apache Tomcat

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    Preparation and properties of whey protein-supported theaflavins nanoparticles
    ZHOU Zheng-jiang, JIANG Xing-jiao, LUO Yan-kai, FANG Chong-ye
    HUBEI AGRICULTURAL SCIENCES    2025, 64 (3): 122-128.   DOI: 10.14088/j.cnki.issn0439-8114.2025.03.020
    Abstract25)      PDF (2819KB)(3)       Save
    To improve the utilization rate of theaflavins, the reverse-solvent method was used to prepare whey protein-theaflavins(WP/TF) nanoparticles. The embedding rate and stability constant were used as indexes. The optimal preparation process was determined by single factor and orthogonal tests, and the performance of particle size, Zeta potential, gastrointestinal simulated digestion and storage stability were studied. The results showed that the optimum preparation process for whey protein-theaflavins nanoparticles was the WP/TF mass ratio of 10∶1, hydration temperature of 55 ℃, and hydration time of 1.5 h. The resulting nanoembedding rate was 85.89%, particle size was (557.05±32.55) nm, Zeta potential was (-58.83±3.05) mV, and the resolubility and water solubility were good. Under simulated gastrointestinal digestion, the TF release rate of nanoparticles was significantly reduced, and it had a good slow release effect. After 30 days of storage at 4 ℃ and 25 ℃, the inclusion rate decreased, but the inclusion rate remained above 70% at 4 ℃. Therefore, whey protein-theaflavins nanoparticles exhibited good stability and slow release performance and could improve the utilization rate of theaflavins, which was highly important for the development and utilization of theaflavins.
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    Effects of ozone on the quality of over aged tobacco raw materials
    YE Wei-min, LIU Yong, HUANG Lei, YIN Peng
    HUBEI AGRICULTURAL SCIENCES    2024, 63 (12): 139-144.   DOI: 10.14088/j.cnki.issn0439-8114.2024.12.026
    Abstract128)      PDF (1910KB)(4)       Save
    In order to study the effect of ozone on the quality of over aged tobacco raw materials and solve the problem of over aged tobacco mold, two sets of different ozone concentration levels (0、50、100、150 μmol/mol; 0、100、200、300 μmol/mol) and three ozone treatment times (30、60、90 days) were set up to study the effects of ozone on the anti mold effect, color and quality of aged tobacco leaves for 3 and 7 years. The results showed that treating tobacco leaves with 200 μmol/mol ozone for 60 days could kill mold and remove most of the moldy odor. After 90 days of treating tobacco leaves with 50~150 μmol/mol ozone, the color transition to brownish black was significantly inhibited. For tobacco leaves that had been aged for 3 years, ozone treatment would have a significant negative effect, resulting in poor quality. For tobacco leaves that had been aged for 7 years, ozone treatment would have a significant positive effect, and 100 μmol/mol ozone had the best effect on improving the quality of tobacco leaves. Therefore, ozone could improve the quality of excessively aged tobacco raw materials, increase their utilization value, and effectively eliminate tobacco leaf mold, solving the problem of tobacco raw material mold. However, for tobacco leaves that had just passed the optimal aging period, ozone treatment should not be used.
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